PHLEBOTOMY CERTIFICATION EXAM
QUESTIONS WITH 100% CORRECT
ANSWERS | LATEST VERSION 2025/2026.
Coagulation tubes have which color stopper? - ANS light blue
Hematology tubes have which color stopper? - ANS purple/lavender
Serum tubes have which color stopper? - ANS red/tiger top/gold
A health care-acquired infections are called? - ANS Nosocomial infections
A inherited condition that is caused by the body's inability to metabolize phenylalanine
(protein) is called: - ANS PKU
A lavender stoppered evacuated tube must be redrawn because of clots in the tube. What
collection error causes this? - ANS insufficient mixing of additive with specimen
A patient has undergone a left radical mastectomy. What site can not be used for venipuncture
until the patient's physician is consulted? - ANS left arm
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,A patient suffers a transfusion reaction. What may this condition cause in the patient's
specimen? - ANS hemolysis
A phlebotomist is to collect a specimen for an APTT and prothrombin time from a patient.
According to the NCCLS guidelines, what tube stopper color should the phlebotomist collect
FIRST? - ANS red
A phlebotomist enters a patient's room at 5:30 am, but the patient is asleep. The phlebotomist
should: - ANS gently wake the patient and proceed
A phlebotomist is about to collect a blood specimen from a patient in ICU, but the patient is
unconscious. The phlebotomist should: - ANS proceed as though the patient were conscious
After performing a routine venipuncture, the phlebotomist noticed that the blood in the
evacuated tube was bright cherry red. What vessel did the phlebotomist puncture? -
ANS artery
After puncturing a finger during a skin puncture procedure, what is the next step? -
ANS Wipe away the first drop with gauze.
Agranulocytes that function in antibody production are called: - ANS lymphocytes
An evacuated tube contains clot activator and serum separator gel. What color is the Hemogard
TM closure? - ANS gold
An example of a specimen that requires protection from light is: - ANS Billirubin, beta-
carotene and porphyrins.
Anti diuretic hormone (ADH) requires which of the following blood collection tubes for proper
test results? - ANS purple
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, At least how long should a warmer be applied to a skin puncture site prior to making a skin
puncture? - ANS 3 minutes
Before starting a bleeding time test, the phlebotomist should: - ANS ask the patient if he or
she has taken any medications
Bleeding time is used to access what? - ANS platelet function, blood vessel integrity
Bleeding time procedures are usually ordered: - ANS pre-surgically
Blood collection for neonatal screening to detect metabolic and genetic abnormalities usually
occurs: - ANS by collecting from the lateral plantar surface of the heel.
Blood enters the right side of the heart from what blood vessel? - ANS superior vena cava
Blood enters the left side of the heart from what blood vessel? - ANS pulmonary vein
Blood exits the heart from the left ventricle through what valve? - ANS aortic
Blood exits the heart from the right atrium through what valve? - ANS tricuspid valve
Blood from a routine venipuncture should have what color? - ANS dark red
Circulating blood cells normally include which of the following: - ANS RBCs, WBCs, platelets.
Deoxygenated blood is found in which blood vessels? - ANS veins
3 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED
QUESTIONS WITH 100% CORRECT
ANSWERS | LATEST VERSION 2025/2026.
Coagulation tubes have which color stopper? - ANS light blue
Hematology tubes have which color stopper? - ANS purple/lavender
Serum tubes have which color stopper? - ANS red/tiger top/gold
A health care-acquired infections are called? - ANS Nosocomial infections
A inherited condition that is caused by the body's inability to metabolize phenylalanine
(protein) is called: - ANS PKU
A lavender stoppered evacuated tube must be redrawn because of clots in the tube. What
collection error causes this? - ANS insufficient mixing of additive with specimen
A patient has undergone a left radical mastectomy. What site can not be used for venipuncture
until the patient's physician is consulted? - ANS left arm
1 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED
,A patient suffers a transfusion reaction. What may this condition cause in the patient's
specimen? - ANS hemolysis
A phlebotomist is to collect a specimen for an APTT and prothrombin time from a patient.
According to the NCCLS guidelines, what tube stopper color should the phlebotomist collect
FIRST? - ANS red
A phlebotomist enters a patient's room at 5:30 am, but the patient is asleep. The phlebotomist
should: - ANS gently wake the patient and proceed
A phlebotomist is about to collect a blood specimen from a patient in ICU, but the patient is
unconscious. The phlebotomist should: - ANS proceed as though the patient were conscious
After performing a routine venipuncture, the phlebotomist noticed that the blood in the
evacuated tube was bright cherry red. What vessel did the phlebotomist puncture? -
ANS artery
After puncturing a finger during a skin puncture procedure, what is the next step? -
ANS Wipe away the first drop with gauze.
Agranulocytes that function in antibody production are called: - ANS lymphocytes
An evacuated tube contains clot activator and serum separator gel. What color is the Hemogard
TM closure? - ANS gold
An example of a specimen that requires protection from light is: - ANS Billirubin, beta-
carotene and porphyrins.
Anti diuretic hormone (ADH) requires which of the following blood collection tubes for proper
test results? - ANS purple
2 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED
, At least how long should a warmer be applied to a skin puncture site prior to making a skin
puncture? - ANS 3 minutes
Before starting a bleeding time test, the phlebotomist should: - ANS ask the patient if he or
she has taken any medications
Bleeding time is used to access what? - ANS platelet function, blood vessel integrity
Bleeding time procedures are usually ordered: - ANS pre-surgically
Blood collection for neonatal screening to detect metabolic and genetic abnormalities usually
occurs: - ANS by collecting from the lateral plantar surface of the heel.
Blood enters the right side of the heart from what blood vessel? - ANS superior vena cava
Blood enters the left side of the heart from what blood vessel? - ANS pulmonary vein
Blood exits the heart from the left ventricle through what valve? - ANS aortic
Blood exits the heart from the right atrium through what valve? - ANS tricuspid valve
Blood from a routine venipuncture should have what color? - ANS dark red
Circulating blood cells normally include which of the following: - ANS RBCs, WBCs, platelets.
Deoxygenated blood is found in which blood vessels? - ANS veins
3 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED
