WGU Biochemistry Final Review
2025/2026 Exam Questions and Answers
| 100% Solved
1. The polymerase chain reaction is a tool used to study protein structure.
True
False - 🧠 ANSWER ✔✔False
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PCR is a tool used to amplify a specific segment of DNA.
What color is the primer in the following diagram?
Red
,Purple
Blue - 🧠 ANSWER ✔✔Red
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Recall from the section on DNA replication that DNA polymerase needs a
primer to begin DNA synthesis. This requirement means the primers will
direct the DNA polymerase to only synthesize complementary strands of
the target DNA. (Note: In DNA replication, the primers are RNA primers,
while PCR generally uses DNA primers because they are more stable.)
dNTPs are DNA nucleotides used in PCR.
True
False - 🧠 ANSWER ✔✔True
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dNTPs stands for deoxynucleotide triphosphates, which are the nucleotides
used in DNA synthesis.
,A thermocycler is a machine used for PCR that varies the temperature of a
sample.
True
False - 🧠 ANSWER ✔✔True
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For PCR, in each cycle, the two strands of the duplex DNA are separated
by heating, then the reaction mixture is cooled to allow the primers to
anneal (or pair) to their complementary segments on the DNA. Next, the
DNA polymerase directs the synthesis of the complementary strands. The
use of a heat-stable DNA polymerase eliminates the need to add fresh
enzyme after each round of heating (heat inactivates most enzymes).
Hence, in the presence of sufficient quantities of primers and dNTPs, PCR
is carried out simply by cycling through the different temperatures for strand
separation, primer annealing, and DNA synthesis.
The thermocycler is the machine that is used to vary the temperature of the
samples.
COPYRIGHT©NINJANERD 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE. PRIVACY
STATEMENT. ALL RIGHTS RESERVED
3
, DNA polymerase is used in DNA replication and in PCR.
True
False - 🧠 ANSWER ✔✔True
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Both PCR and DNA replication require DNA polymerase to make new
copies of DNA.
Which of the following changes can NOT be detected using PCR?
Differences in DNA sequence
Epigenetic changes
Deletions
Insertions - 🧠 ANSWER ✔✔Epigenetic changes
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2025/2026 Exam Questions and Answers
| 100% Solved
1. The polymerase chain reaction is a tool used to study protein structure.
True
False - 🧠 ANSWER ✔✔False
Feedback
PCR is a tool used to amplify a specific segment of DNA.
What color is the primer in the following diagram?
Red
,Purple
Blue - 🧠 ANSWER ✔✔Red
Feedback
Recall from the section on DNA replication that DNA polymerase needs a
primer to begin DNA synthesis. This requirement means the primers will
direct the DNA polymerase to only synthesize complementary strands of
the target DNA. (Note: In DNA replication, the primers are RNA primers,
while PCR generally uses DNA primers because they are more stable.)
dNTPs are DNA nucleotides used in PCR.
True
False - 🧠 ANSWER ✔✔True
Feedback
dNTPs stands for deoxynucleotide triphosphates, which are the nucleotides
used in DNA synthesis.
,A thermocycler is a machine used for PCR that varies the temperature of a
sample.
True
False - 🧠 ANSWER ✔✔True
Feedback
For PCR, in each cycle, the two strands of the duplex DNA are separated
by heating, then the reaction mixture is cooled to allow the primers to
anneal (or pair) to their complementary segments on the DNA. Next, the
DNA polymerase directs the synthesis of the complementary strands. The
use of a heat-stable DNA polymerase eliminates the need to add fresh
enzyme after each round of heating (heat inactivates most enzymes).
Hence, in the presence of sufficient quantities of primers and dNTPs, PCR
is carried out simply by cycling through the different temperatures for strand
separation, primer annealing, and DNA synthesis.
The thermocycler is the machine that is used to vary the temperature of the
samples.
COPYRIGHT©NINJANERD 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE. PRIVACY
STATEMENT. ALL RIGHTS RESERVED
3
, DNA polymerase is used in DNA replication and in PCR.
True
False - 🧠 ANSWER ✔✔True
Feedback
Both PCR and DNA replication require DNA polymerase to make new
copies of DNA.
Which of the following changes can NOT be detected using PCR?
Differences in DNA sequence
Epigenetic changes
Deletions
Insertions - 🧠 ANSWER ✔✔Epigenetic changes
Feedback
