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Biochemistry – WGU – 2025 Study Guide with 100+ Practice Questions and Detailed Answers

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This comprehensive biochemistry study guide for Western Governors University (WGU) includes over 100 practice questions with detailed explanations and correct answers, specifically tailored for the 2025 academic curriculum. The content covers major biochemical concepts necessary for understanding human metabolism, molecular biology, and clinical applications of biochemistry. It is presented in a clear and structured format, ideal for exam preparation, concept review, or supplemental learning. Key areas covered include: Macromolecules: structure and function of carbohydrates, lipids, proteins, and nucleic acids Enzyme kinetics and regulation: including inhibition, activation, and cofactor roles Metabolic pathways: glycolysis, gluconeogenesis, Krebs cycle, oxidative phosphorylation, and associated disorders DNA replication, transcription, and translation, with a focus on genetic expression and regulation Bioenergetics and thermodynamics in biological systems Clinical relevance: such as metabolic diseases, hormone function, and diagnostic biomarkers Molecular tools and techniques commonly applied in lab-based assessments This study guide is ideal for: WGU students enrolled in Biochemistry courses or science-based degree programs Learners in Nursing, Pre-Med, Biology, or Health Sciences who need biochemistry fundamentals Individuals preparing for assessments, competency exams, or seeking efficient exam-ready resources Tutors and instructors looking for a structured Q&A tool to assist students with self-paced or guided review Keywords: WGU biochemistry enzyme kinetics metabolism glycolysis Krebs cycle oxidative phosphorylation DNA replication transcription translation macromolecules amino acids lipids proteins carbohydrates bioenergetics clinical biochemistry metabolic disorders nucleic acids biochemical pathways Western Governors University

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Uploaded on
October 23, 2025
Number of pages
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Written in
2025/2026
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WGU Biochemistry 2025/2026 Exam
Questions and Verified Answers |
Already Graded A+



DNA replication is ___________, which allows each of the two strands to

serve as a _______ for the new strands.




b. semiconservative, template - 🧠 ANSWER ✔✔DNA replication is

semiconservative, meaning that each new duplex has one original (parent)

strand and one new strand. Because the two parent strands are separated

during replication and the base pairing is predictable, each parent strand

can serve as a template for the new strand synthesis.

Which of the following enzymes does NOT assist the DNA polymerase on

the lagging strand to overcome its two problems? (Recall that the DNA

polymerase can only make DNA in the 5'->3' direction, and it must bind a

,double-stranded nucleotide polymer before it can start making its own DNA

polymer.)




c. Helicase - 🧠 ANSWER ✔✔Helicase unwinds the double stranded DNA to

allow for replication, but this is not a problem for the DNA polymerase.

Several components of cigarette smoke, including benzopyrene, insert

themselves (intercalate) into the DNA and lead to several types of

mutations such as frameshift mutations, including both insertions and

deletion. Which of the following repair pathways would be used to repair

this type of damage?




c. Nucleotide Excision Repair - 🧠 ANSWER ✔✔Nucleotide excision repair

is used to repair deletions, insertions, and helix-distorting lesions, such as

thymine dimers.

Maternal smoking during pregnancy is hazardous yet common in many

places. Many studies have associated prenatal smoking to unhealthy

physical and psychological outcomes for the baby. Researchers know that

,maternal smoking affects are epigenetic in nature. Which of the following

events can be considered epigenetic in nature?




a. Changes in chromatin structure - 🧠 ANSWER ✔✔Frame shift mutations

are a kind of mutations which result from addition of deletion of a nucleotide

base resulting in an altered reading frame and ultimately a different protein,

than the one the gene originally encoded. Frameshift mutations are genetic

changes because they alter the DNA sequence, whereas epigenetic

changes do not alter the DNA sequence. Epigenetic changes are

modifications to genomic structure (not sequence) that are caused by the

external environment. These environmental factors affect the overall

chromatin structure to allow more or less "access" to the DNA by gene

expression machinery to turn the genes "on" or "off". In other words,

epigenetics can alter gene expression without changing the underlying

DNA sequences. The changes may or may not be heritable, depending on

the location and circumstances.

Blood type is an example of what type of inheritance?




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, a. Codominance - 🧠 ANSWER ✔✔The genes that produce the A and B

antigen proteins can both be expressed independently, and a heterozygote

(someone with both genes) will be produce both A and B proteins - neither

will dominate the other. The is an example of codominance.

What is the expected probability that a child will have an autosomal

dominant disease if their father is heterozygous for the allele and their

mother is homozygous for the normal allele?




a. 50% - 🧠 ANSWER ✔✔If D is the disease-conferring dominant allele and

d is the normal allele, the father has the genotype Dd and the mother's

genotype is dd. Each child can only inherit a d allele from their mother, and

they have a 50% chance of inheriting the D allele from their father. As a

result, the expected probability that their child will inherit the disease is

50%.

The physical trait of lip protrusion exhibits a characteristic type of

inheritance, as shown by the pedigree above. What type of inheritance best

describes this inheritance pattern?

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