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light that enters but does not leave a solution
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1 absorbed light 2 light
3 glass filter (monochromator) 4 high energy waves
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Terms in this set (166)
an energy source that can be characterized in terms
light of wavelength, amplitude and frequency
-the visible spectrum
, the distance between waves
wavelength
the height of a wave
amplitude
how many waves pass in one second (related to
frequency
speed)
high frequency, very short and
compact wavelengths
high energy waves
-e.g. violet
low frequency, long
wavelengths
low energy waves
-e.g. red
the portion of the
electromagnetic spectrum
visible spectrum
from red (lowest energy) to
violet (highest energy)
transmitted light light that is able to pass through a solution
absorbed light light that enters but does not leave a solution
light Transmittance (T) and absorbance have a
_____________________ relationship
inverse (fill in the blank)
-the more light that is absorbed, the less light there
is left over to transmit
, A=abc
-A= absorbance (calculated, no units)
-a= molar absorptivity: constant for a compound
(L/mol*cm)
beer's law -b= light path through the solution: constant
because same cuvette is used (cm)
-c= concentration of substance: value that is
responsible for changes in absorbance because its
the only non-constant (mol/L)
absorbance and concentration have a _______________
proportional relationship
directly (fill in the blank)
-the higher the absorbance in a sample, the higher
the concentration of absorbing substance was
-light source
-entrance slit: ensures no
excess light is able to enter
-monochromator: isolates the
particular wavelengths you
want to measure
-exit slit
-sample contained (cuvette):
spectrophotometer determines the constant b
(path of light through solution)
in beers law
-photodetector: converts light
energy photons into electrical
energy
-readout device: generates a
voltage signal that measures
how much light was detected
colored glass that absorbs and transmits particular
glass filter wavelengths of light
(monochromator) -broad bandpass (50 nm)
-cheap
a set of mirrored surfaces that bounce light back
interference filter and forth to produce constructive interference to
(monochromator) produce desired bandpass
-5-10 nm bandpass (pretty narrow)