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Biochem Exam 4 Questions With Complete Solutions

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Biochem Exam 4 Questions With Complete Solutions

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Biochem
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Biochem











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Institution
Biochem
Course
Biochem

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Uploaded on
October 21, 2025
Number of pages
34
Written in
2025/2026
Type
Exam (elaborations)
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Biochem Exam 4 Questions With Complete Solutions


The tenth reaction in glycolysis ________ (is/is not)
thermodynamically reversable
is Not thermodynamically reversable
The delta G for the tenth reaction in glycolysis is ________
G<<0
The tenth reaction in glycolysis ________ (is/is not) a regulated
step
Is a regulated step
The tenth step is a regulated step because ________
of the functions and roles of pyruvate
The functions and roles of pyruvate are ________
it can be converted to AcCoA for further oxidation, to lactate to
regenerate NAD+, to alanine by transamination, and is the
starting material to make glucose via gluconeogenesis
Glycolysis recap- Energy investment Phase
"• reactions 1 to 5
•invest energy of 2 ATP
•cleave 6-carbon sugar into two 3-carbon sugars
•has "rate-determining" step of the pathway"
Glycolysis recap- Energy Generation Phase

,"•reactions 6 to10
•1 oxidation-reduction reaction that produces 1 NADH per
glyceraldehyde-3-P
•substrate-phosphorylation reactions that produce 2 ATP per
glyceraldehyde-3-P
•two G3P converted to two pyruvate for a total of 2 NADH and
4 ATP "
"Be able to Describe differences between the energy investment
phase and energy generation phase, Know which enzymes are
regulated, Know overall pathway reaction
The regulated enzymes in glycolysis are
* CB panopto Week 2
Free energy was determined using ________
Rabit skeletal muscle
When looking at energy changes inside a cell it is important to
look at ________ (what instead of what)
Free energy changes instead of standard free energy
The overall delta G of glycolysis is _________
<0
The glycolysis pathway is ____________
(favorable/unfavorable)
Favorable
All metabolic pathways are __________ (favorable/unfavorable)
Favorable

,Glycolysis is a(n) ________ (anabolic or catabolic)
Catabolic pathway
The Regulated enzymes are ___________
Hexokinase, PFK1, Pyruvate kinase
The thermodynamically irreversible steps are also the
_________
Regulated steps
Most of the Glycolysis steps are _______
Freely reversible
Since most of the glycolysis reactions are freely reversible
__________ (what does this mean?)
Most of the enzymes in glycolysis will also be used in
gluconeogenesis
delta G = ________
Delta G = delta G' + RT*ln([P]/[S])
Being that in glycolysis there are three thermodynamically
irreversible reactions ________ (what does this tell us?)
In gluconeogenisis there will need to be other enzymes to go in
the reverse direction
Term
1
Definition

, Glycolysis
Location




Term

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