AAB Medical Technologist Chemistry Exam | 2025/2026
Practice Questions and Study Material
Which of the four elements is proteins differentiates this class substances from
carbohydrates and lipids?
A. carbon
B. hydrogen
C. nitrogen
D. oxygen - correct answerC. Nitrogen
Which of the proteins is soluble in water?
A. albumin
B. alpha-globulins
C. beta-globulins
D. gamma-globulins - correct answerA. Albumin
How are amino acids joined together to form a chainlike structure?
A. disulfide bonding
B. hydrogen bonding
C. covalent bonding
D. peptide bonding - correct answerD. Peptide bonding
Proteins may become denatured when subjected to mechanical agitation, heat, or
chemical treatment. What does denaturation of proteins refer to?
A. amino acid substitution
B. addition of a prosthetic group
C. alteration in tertiary structure
D. increase in solubility - correct answerC. Alteration in tertiary structure
Which aminoacidopathy is NOT caused by an enzyme deficiency?
A. maple syrup substitution
B. phenylketonuria
C. alkaptonuria
D. cystinuria - correct answerD. Cystinuria
How many immunoglobulin classes are currently recognized?
A. two
B. three
,C. four
D. five - correct answerD. Five
Which reagent is employed in the serum protein determination?
A. biuret
B. molybdenum blue
C. ferricferrocyanide
D. diacetyl monoxime - correct answerA. Biuret
Why should hemolyzed serum not be used for total protein analysis?
A. hemoglobin is absorbed at the same wavelength as protein in the reagent
B. there are elevated protein amounts in red blood cells as compared to serum
C. hemoglobin reacts with biuret reagent
D. the value will be falsely lowered - correct answerA. Hemoglobin is absorbed at the
same wavelength as protein in the reagent
Which of the following is NOT true of the biuret reaction?
A. follows Beer's Law for a reasonable range of protein
B. depends on the presence of at least two peptide linkages
C. is relatively free from interference by lipids and hemoglobin
D. is insensitive to the low protein levels of urine - correct answerC. is relatively free
from interference by lipids and hemoglobin
Why is bromcresol purple the preferred indicator for albumin dye binding techniques?
A. it lends itself to manual and automated procedures
B. it never bind with alpha or beta globulins
C. it can be employed with both serum and plasma
D. there is less interference from pigment(s) - correct answerD. there is less
interference from pigment(s)
What is the most anodic protein on electrophoresis at pH 8.6?
A. albumin
,B. alpha-globulins
C. beta-globulins
D. gamma-globulins - correct answerA. albumin
The total protein is 7.2. The serum protein results are as follows:
Albumin 53% 3.8 g/dL
Alpha-1 3.6% 0.3 g/dL
Alpha-2 11% 0.8 g/dL
Betta 17.6% 1.2 g/dL
Gamma 14.8% 1.1 g/dL
What is the A/G ratio?
A. 3.4
B. 1.6
C. 1.2
D. 1.1 - correct answerD. 1.1
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad
gamma is seen in which disease state?
A. acute inflammation
B. monoclonal gammopathy
C. active cirrhosis
D. nephrotic syndrome - correct answerC. Active cirrhosis
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4%-10%
beta-globulin, and 45% gamma globulin, then what condition may exist in the patient?
A. cirrhosis
B. inflammation
C. nephrotic syndrome
D. monoclonal gammopathy - correct answerD. Monoclonal gammopathy
Glycoproteins and mucoproteins are usually bound to which substance?
A. lipids
B. nucleic acids
C. carbohydrates
D. metals - correct answerC. Carbohydrates
All of the following are glycoproteins EXCEPT which one?
A. ceruloplasmin
B. follicle-stimulating hormone
C. fibrinogen
, D. cryoglobulin - correct answerD. Cryoglobulin
Which one of the following statements is true of albumin?
A. they are temperature-sensitive proteins
B. the specimen should clot at room temperature
C. the specimen is incubated at 22 degrees C for 24 hours
D. only quantitative test are available - correct answerA. They are temperature-sensitive
proteins
Which of the following statements is true of albumin?
A. It makes up the smallest portion of total protein
B. Its size prevents its passage through a damaged glomerulus
C. It is produced in the liver and it is water soluble
D. Clinical problems are usually related to high serum values - correct answerC. It is
produced in the liver and it is water soluble
Decreased alpha-fetoprotein values are associated with which condition?
A. Down syndrome
B. neural tube defects
C. spina bifida
D. cancer - correct answerA. Down syndrome
Which one of the following proteins transports iron?
A. ceruloplasmin
B. hemopexin
C. transferrin
D. haptoglobin - correct answerC. Transferrin
In acute renal failure, which nonprotein nitrogen rises the fastest?
A. uric acid
B. creatinine
C. ammonia
D. blood urea nitrogen - correct answerD. Blood urea nitrogen
By the urease method, urea is enzymatically converted to which end product?
A. ammonia
B. nitrogen
C. allantoin
D. carbon dioxide - correct answerA. Ammonia
Practice Questions and Study Material
Which of the four elements is proteins differentiates this class substances from
carbohydrates and lipids?
A. carbon
B. hydrogen
C. nitrogen
D. oxygen - correct answerC. Nitrogen
Which of the proteins is soluble in water?
A. albumin
B. alpha-globulins
C. beta-globulins
D. gamma-globulins - correct answerA. Albumin
How are amino acids joined together to form a chainlike structure?
A. disulfide bonding
B. hydrogen bonding
C. covalent bonding
D. peptide bonding - correct answerD. Peptide bonding
Proteins may become denatured when subjected to mechanical agitation, heat, or
chemical treatment. What does denaturation of proteins refer to?
A. amino acid substitution
B. addition of a prosthetic group
C. alteration in tertiary structure
D. increase in solubility - correct answerC. Alteration in tertiary structure
Which aminoacidopathy is NOT caused by an enzyme deficiency?
A. maple syrup substitution
B. phenylketonuria
C. alkaptonuria
D. cystinuria - correct answerD. Cystinuria
How many immunoglobulin classes are currently recognized?
A. two
B. three
,C. four
D. five - correct answerD. Five
Which reagent is employed in the serum protein determination?
A. biuret
B. molybdenum blue
C. ferricferrocyanide
D. diacetyl monoxime - correct answerA. Biuret
Why should hemolyzed serum not be used for total protein analysis?
A. hemoglobin is absorbed at the same wavelength as protein in the reagent
B. there are elevated protein amounts in red blood cells as compared to serum
C. hemoglobin reacts with biuret reagent
D. the value will be falsely lowered - correct answerA. Hemoglobin is absorbed at the
same wavelength as protein in the reagent
Which of the following is NOT true of the biuret reaction?
A. follows Beer's Law for a reasonable range of protein
B. depends on the presence of at least two peptide linkages
C. is relatively free from interference by lipids and hemoglobin
D. is insensitive to the low protein levels of urine - correct answerC. is relatively free
from interference by lipids and hemoglobin
Why is bromcresol purple the preferred indicator for albumin dye binding techniques?
A. it lends itself to manual and automated procedures
B. it never bind with alpha or beta globulins
C. it can be employed with both serum and plasma
D. there is less interference from pigment(s) - correct answerD. there is less
interference from pigment(s)
What is the most anodic protein on electrophoresis at pH 8.6?
A. albumin
,B. alpha-globulins
C. beta-globulins
D. gamma-globulins - correct answerA. albumin
The total protein is 7.2. The serum protein results are as follows:
Albumin 53% 3.8 g/dL
Alpha-1 3.6% 0.3 g/dL
Alpha-2 11% 0.8 g/dL
Betta 17.6% 1.2 g/dL
Gamma 14.8% 1.1 g/dL
What is the A/G ratio?
A. 3.4
B. 1.6
C. 1.2
D. 1.1 - correct answerD. 1.1
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad
gamma is seen in which disease state?
A. acute inflammation
B. monoclonal gammopathy
C. active cirrhosis
D. nephrotic syndrome - correct answerC. Active cirrhosis
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4%-10%
beta-globulin, and 45% gamma globulin, then what condition may exist in the patient?
A. cirrhosis
B. inflammation
C. nephrotic syndrome
D. monoclonal gammopathy - correct answerD. Monoclonal gammopathy
Glycoproteins and mucoproteins are usually bound to which substance?
A. lipids
B. nucleic acids
C. carbohydrates
D. metals - correct answerC. Carbohydrates
All of the following are glycoproteins EXCEPT which one?
A. ceruloplasmin
B. follicle-stimulating hormone
C. fibrinogen
, D. cryoglobulin - correct answerD. Cryoglobulin
Which one of the following statements is true of albumin?
A. they are temperature-sensitive proteins
B. the specimen should clot at room temperature
C. the specimen is incubated at 22 degrees C for 24 hours
D. only quantitative test are available - correct answerA. They are temperature-sensitive
proteins
Which of the following statements is true of albumin?
A. It makes up the smallest portion of total protein
B. Its size prevents its passage through a damaged glomerulus
C. It is produced in the liver and it is water soluble
D. Clinical problems are usually related to high serum values - correct answerC. It is
produced in the liver and it is water soluble
Decreased alpha-fetoprotein values are associated with which condition?
A. Down syndrome
B. neural tube defects
C. spina bifida
D. cancer - correct answerA. Down syndrome
Which one of the following proteins transports iron?
A. ceruloplasmin
B. hemopexin
C. transferrin
D. haptoglobin - correct answerC. Transferrin
In acute renal failure, which nonprotein nitrogen rises the fastest?
A. uric acid
B. creatinine
C. ammonia
D. blood urea nitrogen - correct answerD. Blood urea nitrogen
By the urease method, urea is enzymatically converted to which end product?
A. ammonia
B. nitrogen
C. allantoin
D. carbon dioxide - correct answerA. Ammonia
