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Graficas estudio laboratorio

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CROMATOGRAFIA POR FILTRAION DE GEL. SEPARACIÓN DE HEMOGLOBINA Y ALANINA EN SEPHADEX G-25. ESTIMACION CUATITATIVA DE PROTEINAS MEDINTE EL METODO BIURET ESTUDIO CINETICO DE LA POLIFENOLOXIDASA

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January 10, 2021
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Written in
2016/2017
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Graficas guio Bioquímica 2017

CROMATOGRAFIA POR FILTRAION DE GEL. SEPARACIÓN DE HEMOGLOBINA Y ALANINA EN
SEPHADEX G-25

Pregunta 2

Absorbancia
VOLUMEN (mL) (A)
3 0
6 0,004
9 0,006
12 0,009 azul dextrato + cromato potasico
15 0,106
0.12
18 0,0105
21 0,001 0.1
24 0,003
Absorbancia (A)



0.08
27 0,007
30 0,014 0.06
33 0,017 0.04
36 0,018
39 0,011 0.02

42 0,007 0
0 10 20 30 40 50 60
45 0,006
48 0,003 Volumen (mL)
51 0,002
54 0,001


absorbanci
volumen a
3 0
6 0,001
9 0,002 Hemoglobina + Alanina
12 0,003 1
15 0,004 0.9
18 0,027 0.8
21 0,021 0.7
Absorbancia (A)




24 0,013 0.6
0.5
27 0,001
0.4
30 0,375
0.3
33 0,792 0.2
36 0,868 0.1
39 0,707 0
0 10 20 30 40 50 60
42 0,03
45 0,009 Volumen (mL)
48 0,008
51 0,003
54 0,001
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