Pearson Edexcel Level 3 GCE Biology B Advanced PAPER 1: Advanced Biochemistry, Microbiology and Genetics QP JUNE 2025

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Paper


B 🟐 🟐


Advanced
PAPER 1: Advanced Biochemistry, Microbiology
and Genetics



Pearson Edexcel Level 3 GCE Biology B Advanced PAPER 1: Advanced Biochemistry, Microbiology and Genetics
QP JUNE 2025
Instructions

•• Use black ink or ball-point pen.
Fill in the boxes at the top of this page with your name,
centre number and candidate number.
•• Answer all questions.
Answer the questions in the spaces provided
– there may be more space than you need.

Information

•• The total mark for this paper is 90.
The marks for each question are shown in brackets

• toIn structure
– use this as a guide as to how much time to spend on each question.
question(s) marked with an asterisk (*), marks will be awarded for your ability
your answer logically showing how the points that you make are
related or follow on from each other where appropriate.

Advice
• Read each question carefully before you start to answer it.
• Try to answer every question.
• Check your answers if you have time at the end.
Turn over


P74459A
©2025 Pearson Education Ltd.
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, Answer ALL questions.

Some questions must be answered with a cross in a box . If you change your mind about an




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answer, put a line through the box and then mark your new answer with a cross .
1 Active transport is one type of cell transport mechanism.
(a) What happens during active transport?
(1)
A ATP is hydrolysed releasing energy

B ATP is hydrolysed using energy

C ATP is phosphorylated releasing energy

D ATP is phosphorylated using energy


(b) Which substance is transported against a concentration gradient by active
transport?
(1)




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A hydrophilic molecule

B large hydrophobic molecule

C small hydrophobic molecule

D water




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, (c) The graph shows the change in concentration of molecule A and molecule B, as
they are transported into the cytoplasm of a cell.
molecule A
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Concentration of molecules
in the cytoplasm molecule B




0 10 20 30
Time / mins

Explain the differences in the changes in concentration of molecule A and
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molecule B between 0 and 10 minutes and between 20 and 30 minutes.
(2)

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.. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . ......................



.. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . ......................



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(Total for Question 1 = 4 marks)
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, 2 The diagram shows part of cellular respiration.

glucose




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glycolysis


molecule X
link reaction
and
Krebs cycle
carbon dioxide

(a) Which is molecule X?
(1)

A glycerate 3-phosphate
B glycogen




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C lactate

D pyruvate


(b) Which row of the table shows the locations of glycolysis, the link reaction and the
Krebs cycle in eukaryotic cells?
(1)

Glycolysis Link reaction Krebs cycle

A cytoplasm cytoplasm cytoplasm

B cytoplasm mitochondria mitochondria
C mitochondria cytoplasm cytoplasm
D mitochondria mitochondria mitochondria DO NOT WRITE IN THIS AREA




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