Complete BOC: ASCP prep
1) After receiving a 24 hr. Urine for quantitative general protein evaluation the technician must
first:
a. Way of life the urine for bacteria
b. Add the correct preservative
c. Screen for albumin the usage of a dipstick
d. Degree the whole extent - ANS-d (degree the whole volume)
1) Following overnight fasting, hypoglycemia in adults is defined as a glucose of:
a. <70 mg/dL (<three.9 mmol/L)
b. <60 mg/dL (<3.3mmol/L)
c. <55 mg/dL (<3.0mmol/L)
d. <45mg/dL (<2.5mmol/L) - ANS-d (Diagnosis of hypoglycemia in adults.)
1) Proper media for culture of a urethral discharge from a man include:
a. Sheep blood and phenylethyl alcohol agars
b. Eosin methylene blue and sheep blood agars
c. Thioglycollate broth and chocolate agar
d. Chocolate agar and modified Thayer Martin agar - ANS-d (Chocolate agar and chocolate
agar-based selective media should be used for recovery of Neisseria gonorrhoeae from urethral
discharge. Chocolate agar provides the nutrients required by N gonorrhoeae and selective
media contains antimicrobial agents that inhibits other organisms and permits recovery of
pathogenic Neisseria.)
1) The light-colored zone adjacent to the nucleus in a plasmacyte is the:
a. Ribosome
b. Chromatin
c. Mitochondria
d. Golgi area - ANS-d (Morphological identifiable perinuclear halo.)
1) The minimum Hgb concentration in a fingerstick from a male blood donor is:
a. 12.0 g/dL (120 g/L)
b. 12.5 g/dL (125 g/L)
c. 13.5 g/dL (135 g/L)
d. 15.0 g/dL (150 g/L) - ANS-b (All donors, regardless of sex, require a minimum hemoglobin of
12.5 g/dL [125 g/L]. The value must not be performed on an earlobe stick.)
10) A 45 yr. Old woman has a fasting serum glucose concentration of 95 mg/dL (5.2 mmol/L)
and a 2 hr. Postprandial glucose concentration of 105 mg/dL (5.8 mmol/L). The statement which
best describes this patient's fasting serum glucose concentration is:
a. Normal; reflecting glycogen breakdown by the liver
,b. Normal; reflecting glycogen breakdown by skeletal muscle
c. Abnormal; indication diabetes mellitus
d. Abnormal; indicating hypoglycemia - ANS-a (Factors contributing to PBS.)
10) During the preparation of Platelet Concentrates from whole blood, the blood should be:
a. Cooled towards 6 C
b. Cooled towards 20-24 C
c. Warmed to 37 C
d. Heated to 57 C - ANS-b (Platelets are prepared and stored at 20-24 C for optimum function.)
10) Failure to observe RBC casts in a urine specimen can be caused by:
a. Staining the specimen
b. Centrifuging an unmixed specimen
c. Mixing the sediment after decantation
d. Examining the sediment first under low power - ANS-b (centrifuging an unmixed specimen)
10) In order for hemoglobin to combine reversibly with oxygen, the iron must be:
a. Complexed with haptoglobin
b. Freely circulating in the cytoplasm
c. Attached to transferrin
d. In the ferrous state - ANS-d (Functional form of Fe [reduced].)
10) The mean value of a series of hemoglobin controls was found to be 15.2 g/dL, and the
standard deviation was calculated at 0.20. Acceptable control range for the laboratory is +/- 2
standard deviations. Which of the following represents the allowable limits for the control?
A. 14.5 - 15.5 g/dL
b. 15.0 - 15.4 g/dL
c. 15.2 - 15.6 g/dL
d. 14.8 - 15.6 g.DL - ANS-d (Standard deviation is a measure of the dispersion of data around
the mean.)
10) Which selective medium is used for the isolation of gram-positive microorganisms?
A. Columbia CNA with 5% sheep blood
b. Trypticase soy agar with 5% sheep blood
c. Eosin methylene blue
d. Modified Thayer-Martin - ANS-a (Columbia CNA agar contains colistin and nalidixic acid,
which inhibit most gram-negative organisms. Eosin methylene blue is selective and inhibits
gram-positive organisms and modified Thayer Martin is selective and inhibits gram-positive
organisms, gram-negative bacilli and yeast.)
100) Gram-positive cocci in chains are seen on a Gram stain from a blood culture. The
organism grows as a beta-hemolytic colony. Further tests that could be performed include:
a. Bile esculin, PYR, bacitracin, and hippurate
b. Catalase and coagulase
,c. Oxidase and deoxyribonuclease
d. Voges-Proskauer and methyl red - ANS-a (Bile esculin, PYR, bacitracin and hippurate are
biochemicals/tests used in the presumptive or definitive identification of beta-hemolytic
streptococci such as S pyogenes, S agalactiae and Enterococcus.)
100) The first step in the quantitation of serum iron is:
a. Direct reaction with appropriate chromogen
b. Iron saturation of transferrin
c. Free iron precipitation
d. Separation of iron from transferrin - ANS-d (Principle of method of analysis.)
100) The lab findings on a patient are as follows:
MCV = 55 um
MCHC = 25%
MCH = 17 pg
A stained blood film of this patient would most likely reveal a red cell picture that is:
a. Microcytic, hypochromic
b. Macrocytic, hypochromic
c. Normocytic, normochromic
d. Microcytic, normochromic - ANS-a (Using RBC indices to classify anemia.)
100) To distinguish between a clump of WBCs and a WBC cast it is important to observe:
a. The presence of free floating WBCs
b. A positive leukocyte reaction
c. A positive nitrite reaction
d. The presence of a cast matrix - ANS-d (the presence of a cast matrix)
101) "Nutritionally deficient" streptococci are:
a. Enterococci
b. Group D nonenterococci
c. Cell wall-deficient streptococci
d. In the genera Granulicatella and Abiotrophia - ANS-d (Organisms that used to be categorized
as nutritionally variant or deficient streptococci have been reclassified into the genera
Abiotrophia and Granulicatella.)
101) A patient has the following laboratory results:
The mean corpuscular volume (MCV) of the patient is:
a. 35 mm3 (35 fL)
b. 83 mm3 (83 fL)
c. 120 mm3 (120 fL)
d. 150 mm3 (150 fL - ANS-c (Calculating RBC indices.)
101) Spherical urothelial cells may be confused with:
a. Oval fat bodies
, b. Renal tubular epithelial cell
c. Glitter cells
d. Lymphocytes - ANS-b (renal tubular epithelial cell)
102) After 24 hrs. A blood culture from a newborn grows catalase-negative, gram-positive cocci.
The bacterial colonies are small, translucent and beta-hemolytic on a blood agar plate.
Biochemical test results of a pure culture are:
bacitracin: resistant
CAMP reaction: positive
bile esculin: not hydrolyzed
6.5% NaCl broth: no growth
Assuming that all controls react properly and reactions are verified, the next step would be to:
a. Perform a Streptococcus group typing
b. Report the organism as Streptococcus pneumoniae
c. Report the organism as Staphylococcus aureus
d. Report the organism as Staphylococcus epidermidis - ANS-a (The colony description and
biochemical results presented describe Streptococcus agalactiae. The identification of this
organism is confirmed by streptococcus antigen typing.)
102) Prior to reporting a RBC cast, it is important to observe:
a. Free floating RBCs
b. Hyaline casts
c. Granular casts
d. Increased WBCs - ANS-a (free-floating RBCs)
102) The following results were obtained from a post-surgical patient receiving total parenteral
nutrition:
The most consistent explanation for the above data is:
a. Acute surgical bleeder
b. Specimen on day 19 from wrong patient
c. Improperly mixed specimen on day 19
d. Lipid interference on days 17 and 18 - ANS-d (Any turbidity in the cyanmethemoglobin
reagent- patient specimen will result in falsely elevated values.)
103) A beta-hemolytic streptococcus that has been isolated from an ear culture grows up to the
edge of a 0.04 unit bacitracin disk. Which of the following tests would help to determine if the
organism is Enterococcus?
A. Growth in 6.5% NaCl broth
b. Growth in the presence of penicillin
c. Optochin susceptibility
d. Fermentation of 10% lactose - ANS-a (Of the biochemicals listed, only growth in 6.5% NaCl
will aid in the identification of Enterococcus, which has the ability to grow in the presence of high
salt concentrations.)
1) After receiving a 24 hr. Urine for quantitative general protein evaluation the technician must
first:
a. Way of life the urine for bacteria
b. Add the correct preservative
c. Screen for albumin the usage of a dipstick
d. Degree the whole extent - ANS-d (degree the whole volume)
1) Following overnight fasting, hypoglycemia in adults is defined as a glucose of:
a. <70 mg/dL (<three.9 mmol/L)
b. <60 mg/dL (<3.3mmol/L)
c. <55 mg/dL (<3.0mmol/L)
d. <45mg/dL (<2.5mmol/L) - ANS-d (Diagnosis of hypoglycemia in adults.)
1) Proper media for culture of a urethral discharge from a man include:
a. Sheep blood and phenylethyl alcohol agars
b. Eosin methylene blue and sheep blood agars
c. Thioglycollate broth and chocolate agar
d. Chocolate agar and modified Thayer Martin agar - ANS-d (Chocolate agar and chocolate
agar-based selective media should be used for recovery of Neisseria gonorrhoeae from urethral
discharge. Chocolate agar provides the nutrients required by N gonorrhoeae and selective
media contains antimicrobial agents that inhibits other organisms and permits recovery of
pathogenic Neisseria.)
1) The light-colored zone adjacent to the nucleus in a plasmacyte is the:
a. Ribosome
b. Chromatin
c. Mitochondria
d. Golgi area - ANS-d (Morphological identifiable perinuclear halo.)
1) The minimum Hgb concentration in a fingerstick from a male blood donor is:
a. 12.0 g/dL (120 g/L)
b. 12.5 g/dL (125 g/L)
c. 13.5 g/dL (135 g/L)
d. 15.0 g/dL (150 g/L) - ANS-b (All donors, regardless of sex, require a minimum hemoglobin of
12.5 g/dL [125 g/L]. The value must not be performed on an earlobe stick.)
10) A 45 yr. Old woman has a fasting serum glucose concentration of 95 mg/dL (5.2 mmol/L)
and a 2 hr. Postprandial glucose concentration of 105 mg/dL (5.8 mmol/L). The statement which
best describes this patient's fasting serum glucose concentration is:
a. Normal; reflecting glycogen breakdown by the liver
,b. Normal; reflecting glycogen breakdown by skeletal muscle
c. Abnormal; indication diabetes mellitus
d. Abnormal; indicating hypoglycemia - ANS-a (Factors contributing to PBS.)
10) During the preparation of Platelet Concentrates from whole blood, the blood should be:
a. Cooled towards 6 C
b. Cooled towards 20-24 C
c. Warmed to 37 C
d. Heated to 57 C - ANS-b (Platelets are prepared and stored at 20-24 C for optimum function.)
10) Failure to observe RBC casts in a urine specimen can be caused by:
a. Staining the specimen
b. Centrifuging an unmixed specimen
c. Mixing the sediment after decantation
d. Examining the sediment first under low power - ANS-b (centrifuging an unmixed specimen)
10) In order for hemoglobin to combine reversibly with oxygen, the iron must be:
a. Complexed with haptoglobin
b. Freely circulating in the cytoplasm
c. Attached to transferrin
d. In the ferrous state - ANS-d (Functional form of Fe [reduced].)
10) The mean value of a series of hemoglobin controls was found to be 15.2 g/dL, and the
standard deviation was calculated at 0.20. Acceptable control range for the laboratory is +/- 2
standard deviations. Which of the following represents the allowable limits for the control?
A. 14.5 - 15.5 g/dL
b. 15.0 - 15.4 g/dL
c. 15.2 - 15.6 g/dL
d. 14.8 - 15.6 g.DL - ANS-d (Standard deviation is a measure of the dispersion of data around
the mean.)
10) Which selective medium is used for the isolation of gram-positive microorganisms?
A. Columbia CNA with 5% sheep blood
b. Trypticase soy agar with 5% sheep blood
c. Eosin methylene blue
d. Modified Thayer-Martin - ANS-a (Columbia CNA agar contains colistin and nalidixic acid,
which inhibit most gram-negative organisms. Eosin methylene blue is selective and inhibits
gram-positive organisms and modified Thayer Martin is selective and inhibits gram-positive
organisms, gram-negative bacilli and yeast.)
100) Gram-positive cocci in chains are seen on a Gram stain from a blood culture. The
organism grows as a beta-hemolytic colony. Further tests that could be performed include:
a. Bile esculin, PYR, bacitracin, and hippurate
b. Catalase and coagulase
,c. Oxidase and deoxyribonuclease
d. Voges-Proskauer and methyl red - ANS-a (Bile esculin, PYR, bacitracin and hippurate are
biochemicals/tests used in the presumptive or definitive identification of beta-hemolytic
streptococci such as S pyogenes, S agalactiae and Enterococcus.)
100) The first step in the quantitation of serum iron is:
a. Direct reaction with appropriate chromogen
b. Iron saturation of transferrin
c. Free iron precipitation
d. Separation of iron from transferrin - ANS-d (Principle of method of analysis.)
100) The lab findings on a patient are as follows:
MCV = 55 um
MCHC = 25%
MCH = 17 pg
A stained blood film of this patient would most likely reveal a red cell picture that is:
a. Microcytic, hypochromic
b. Macrocytic, hypochromic
c. Normocytic, normochromic
d. Microcytic, normochromic - ANS-a (Using RBC indices to classify anemia.)
100) To distinguish between a clump of WBCs and a WBC cast it is important to observe:
a. The presence of free floating WBCs
b. A positive leukocyte reaction
c. A positive nitrite reaction
d. The presence of a cast matrix - ANS-d (the presence of a cast matrix)
101) "Nutritionally deficient" streptococci are:
a. Enterococci
b. Group D nonenterococci
c. Cell wall-deficient streptococci
d. In the genera Granulicatella and Abiotrophia - ANS-d (Organisms that used to be categorized
as nutritionally variant or deficient streptococci have been reclassified into the genera
Abiotrophia and Granulicatella.)
101) A patient has the following laboratory results:
The mean corpuscular volume (MCV) of the patient is:
a. 35 mm3 (35 fL)
b. 83 mm3 (83 fL)
c. 120 mm3 (120 fL)
d. 150 mm3 (150 fL - ANS-c (Calculating RBC indices.)
101) Spherical urothelial cells may be confused with:
a. Oval fat bodies
, b. Renal tubular epithelial cell
c. Glitter cells
d. Lymphocytes - ANS-b (renal tubular epithelial cell)
102) After 24 hrs. A blood culture from a newborn grows catalase-negative, gram-positive cocci.
The bacterial colonies are small, translucent and beta-hemolytic on a blood agar plate.
Biochemical test results of a pure culture are:
bacitracin: resistant
CAMP reaction: positive
bile esculin: not hydrolyzed
6.5% NaCl broth: no growth
Assuming that all controls react properly and reactions are verified, the next step would be to:
a. Perform a Streptococcus group typing
b. Report the organism as Streptococcus pneumoniae
c. Report the organism as Staphylococcus aureus
d. Report the organism as Staphylococcus epidermidis - ANS-a (The colony description and
biochemical results presented describe Streptococcus agalactiae. The identification of this
organism is confirmed by streptococcus antigen typing.)
102) Prior to reporting a RBC cast, it is important to observe:
a. Free floating RBCs
b. Hyaline casts
c. Granular casts
d. Increased WBCs - ANS-a (free-floating RBCs)
102) The following results were obtained from a post-surgical patient receiving total parenteral
nutrition:
The most consistent explanation for the above data is:
a. Acute surgical bleeder
b. Specimen on day 19 from wrong patient
c. Improperly mixed specimen on day 19
d. Lipid interference on days 17 and 18 - ANS-d (Any turbidity in the cyanmethemoglobin
reagent- patient specimen will result in falsely elevated values.)
103) A beta-hemolytic streptococcus that has been isolated from an ear culture grows up to the
edge of a 0.04 unit bacitracin disk. Which of the following tests would help to determine if the
organism is Enterococcus?
A. Growth in 6.5% NaCl broth
b. Growth in the presence of penicillin
c. Optochin susceptibility
d. Fermentation of 10% lactose - ANS-a (Of the biochemicals listed, only growth in 6.5% NaCl
will aid in the identification of Enterococcus, which has the ability to grow in the presence of high
salt concentrations.)
