MOLECULAR BIOLOGY ASCP BOC QUESTIONS WITH ACCURATE ANSWERS

MOLECULAR BIOLOGY ASCP BOC QUESTIONS WITH ACCURATE ANSWERS Reverse transcriptase would best be described as a: - Correct Answer-RNA-dependent DNA polymerase- Reverse transcriptase synthesizes a DNA molecule from an RNA template. What term best describes the relatedness of sequences, the percent identity or conservation? - Correct Answer-similarity In bioinformatics, similarity refers to the percent identity between sequences. A PCR-based assay is performed to determine the clonality of B cells in a patient using a forward primer specific for the innermost framework region and a reverse primer complimentary to the joining region (of the immunoglobulin heavy chain). The resulting electropherogram was obtained. Assuming all controls worked properly for the assay, what is your interpretation of the results for this patient? - Correct Answer-patient has a monoclonal population of B cells The presence of a distinct peak along with the polyclonal background indicates that at least 1% of the patient's B cells have a monoclonal rearrangement of the Ig heavy chain. What is the best method to rule out the possibility of genomic DNA giving a false-positive result in a reverse transcriptase (RT)PCR reaction?