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BIOCHEM ACS EXAM REVIEW – QUESTIONS
AND VERIFIED ANSWERS 2025/2025
What amino acids have nonpolar, aliphatic R groups?-correct-answer-Glycine,
Alanine, Proline, Valine, Leucine, Isoleucine, Methionine
What amino acids have polar, uncharged R groups?-correct-answer-Serine,
Threonine, Cysteine, Asparagine, and Glutamine
Which active site binding causes a conformational change of the enzyme, induced
fit or lock and key?-correct-answer-Induced fit
What is maximum velocity?-correct-answer-All of the active sites are filled with
substrates
What does V=?-correct-answer-V= Vmax * [S]/ [S] + Km
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What is Km?-correct-answer-Concentration of substrate needed to fill half of the
active sites
In a Lineweaver-Burk plot, what does the y-intercept =?-correct-answer-1/ Vmax
In a Lineweaver-burk plot, what does the x-intercept=?-correct-answer--1/ Km
In a Lineweaver-burk plot, what does the slope=?-correct-answer-Km/ Vmax
What is the Turnover Number of an Enzyme?-correct-answer-Number of substrate
molecules converted into products by an enzyme per unit of time, when the
enzyme is fully saturated with substrate. K2
In competitive inhibition, what happens?-correct-answer-the molecule binds to
the active site. Vmax is not altered and the x-intercept changes
In noncompetitive inhibition, what happens?-correct-answer-the inhibitor binds
somewhere else on the enzyme at the same time the substrate binds. Vmax is
alteredd, y-intercept changes
BIOCHEM ACS EXAM REVIEW – QUESTIONS
AND VERIFIED ANSWERS 2025/2025
What amino acids have nonpolar, aliphatic R groups?-correct-answer-Glycine,
Alanine, Proline, Valine, Leucine, Isoleucine, Methionine
What amino acids have polar, uncharged R groups?-correct-answer-Serine,
Threonine, Cysteine, Asparagine, and Glutamine
Which active site binding causes a conformational change of the enzyme, induced
fit or lock and key?-correct-answer-Induced fit
What is maximum velocity?-correct-answer-All of the active sites are filled with
substrates
What does V=?-correct-answer-V= Vmax * [S]/ [S] + Km
, 2|Page
What is Km?-correct-answer-Concentration of substrate needed to fill half of the
active sites
In a Lineweaver-Burk plot, what does the y-intercept =?-correct-answer-1/ Vmax
In a Lineweaver-burk plot, what does the x-intercept=?-correct-answer--1/ Km
In a Lineweaver-burk plot, what does the slope=?-correct-answer-Km/ Vmax
What is the Turnover Number of an Enzyme?-correct-answer-Number of substrate
molecules converted into products by an enzyme per unit of time, when the
enzyme is fully saturated with substrate. K2
In competitive inhibition, what happens?-correct-answer-the molecule binds to
the active site. Vmax is not altered and the x-intercept changes
In noncompetitive inhibition, what happens?-correct-answer-the inhibitor binds
somewhere else on the enzyme at the same time the substrate binds. Vmax is
alteredd, y-intercept changes
