QIHC study guide – 7 questions well
answered (A+ rated)
fresh cut tissue surface against a glass slide - correct answer ✔✔Touch-preps involve pressing a:
labeled avidin-biotin - correct answer ✔✔An immunohistochemistry labeling method in which a
molecule of avidin is directly labeled with an enzyme is called:
initial fixation in 95 percent alcohol followed by fixation in glutaraldehyde. - correct answer
✔✔Adequate fixation of a kidney biopsy for diagnostic electron microscopy is obtainable with
all of the following except:
in-situ hybridization - correct answer ✔✔Three of the following techniques can be used to show
where a particular enzyme is located in a section or a thin layer of cells. Which technique
cannot localize an enzyme?
epoxy resin - correct answer ✔✔In order to ensure stable sections in the electron beam, tissue
for electron microscopy is embedded in:
glomerulus - correct answer ✔✔In preparing a kidney biopsy for immunofluorescence
procedures, the critical anatomic structure to locate is the:
Bouins solution - correct answer ✔✔All of the following are suitable fixatives for electron
microscopy except:
osmium tetroxide - correct answer ✔✔if glutaraldehyde is the primary fixative for electron
microscopy, the secondary fixative is usually:
, fluorescein isothyocyanate - correct answer ✔✔a common fluorochrome used in
immunohistochemistry is:
aldehydes - correct answer ✔✔Fixatives that tend to mask antigenic sites and hinder
immunohistochemical localization of antigens contain:
DNA degration - correct answer ✔✔Necrosis, karyolysis, karyorrhexis, and autolysis from delay
in analysis and sample preparation can all lead to:
Frozen tissue, frozen sections - correct answer ✔✔The most common tissue processing method
for fluorescence microscopy techniques is:
several types of cells in the patients body - correct answer ✔✔Commercial monoclonal
antibodies are produced by all of the following except:
The fixative must penetrate through the entire sample - correct answer ✔✔The size of a tissue
sample is important for immunohistochemistry because:
immunogen - correct answer ✔✔A molecule which stimulates an immune response is called
a(n):
lower % coefficient of variation - correct answer ✔✔Advantages to using paraffin-embedded
tissue for flow cytometry include all of the following except:
ribonucleic acid - correct answer ✔✔The acronym ?RNA? stands for:
Deoxyribose Nucleic Acid - correct answer ✔✔The acronym DNA stands for
answered (A+ rated)
fresh cut tissue surface against a glass slide - correct answer ✔✔Touch-preps involve pressing a:
labeled avidin-biotin - correct answer ✔✔An immunohistochemistry labeling method in which a
molecule of avidin is directly labeled with an enzyme is called:
initial fixation in 95 percent alcohol followed by fixation in glutaraldehyde. - correct answer
✔✔Adequate fixation of a kidney biopsy for diagnostic electron microscopy is obtainable with
all of the following except:
in-situ hybridization - correct answer ✔✔Three of the following techniques can be used to show
where a particular enzyme is located in a section or a thin layer of cells. Which technique
cannot localize an enzyme?
epoxy resin - correct answer ✔✔In order to ensure stable sections in the electron beam, tissue
for electron microscopy is embedded in:
glomerulus - correct answer ✔✔In preparing a kidney biopsy for immunofluorescence
procedures, the critical anatomic structure to locate is the:
Bouins solution - correct answer ✔✔All of the following are suitable fixatives for electron
microscopy except:
osmium tetroxide - correct answer ✔✔if glutaraldehyde is the primary fixative for electron
microscopy, the secondary fixative is usually:
, fluorescein isothyocyanate - correct answer ✔✔a common fluorochrome used in
immunohistochemistry is:
aldehydes - correct answer ✔✔Fixatives that tend to mask antigenic sites and hinder
immunohistochemical localization of antigens contain:
DNA degration - correct answer ✔✔Necrosis, karyolysis, karyorrhexis, and autolysis from delay
in analysis and sample preparation can all lead to:
Frozen tissue, frozen sections - correct answer ✔✔The most common tissue processing method
for fluorescence microscopy techniques is:
several types of cells in the patients body - correct answer ✔✔Commercial monoclonal
antibodies are produced by all of the following except:
The fixative must penetrate through the entire sample - correct answer ✔✔The size of a tissue
sample is important for immunohistochemistry because:
immunogen - correct answer ✔✔A molecule which stimulates an immune response is called
a(n):
lower % coefficient of variation - correct answer ✔✔Advantages to using paraffin-embedded
tissue for flow cytometry include all of the following except:
ribonucleic acid - correct answer ✔✔The acronym ?RNA? stands for:
Deoxyribose Nucleic Acid - correct answer ✔✔The acronym DNA stands for
