MB (ASCP) EXAM 2025 QUESTIONS AND
ANSWERS
Predominant form of DNA - ANS Right-handed B
DNA and histone interaction is due to what type of bond? - ANS Ionic
What stains to the minor groove of DNA duplex? - ANS SYBR Green
What is the most abundant type of RNA? - ANS Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? - ANS Genome and mRNA
What increases half-life of mRNA? - ANS 5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? - ANS Euchromatin
What is the most common type of eukaryotic DNA? - ANS Satellite DNA
What specimen has the most DNA according to molecular weight? - ANS White Blood Cells
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What amino acids compose histones? - ANS Lysine and Arginine
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, Amino acid that all proteins start with: - ANS Methionine
What protects the DNA strand during replication? - ANS Single-stranded binding protein
Leading Strand - ANS 3' to 5' template
Lagging Strand - ANS 5' to 3' template
What DNA strand is being copied in transcription? - ANS Antisense Strand
First step after newly formed mRNA is made? - ANS Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what enzyme? -
ANS Telomerase
What polymerase has repair activity? - ANS Beta/Epsilon
What polymerase acts on leading strand? - ANS Delta
What polymerase acts on lagging strand? - ANS Alpha
Which polymerase is used for mitochondrial replication and repair? - ANS Gamma
What polymerase binds the 5' end to 3' end of dsDNA? - ANS Topoisomerase
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Which RNA polymerase is mainly for transcription? - ANS RNA Pol II
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, Which polymerase is involved with mRNA synthesis? - ANS RNA Pol II
What inactivates RNAses? - ANS Diethyl pyrocarbonate (DEPC) or bake glassware for 4 to 6
hours at 400C
What type of restriction enzyme recognizes palindromic sites? - ANS Restriction Enzyme
Type II
What type of restriction enzyme lacks methylation activity? - ANS Restriction Enzyme Type II
What is necessary to make cDNA? - ANS Reverse transcriptase
Number of SNPs in a human genome? - ANS 11 billion (10^7)
How many genes (base pairs) are in a human haploid genome? - ANS 3 billion base pairs
The polymerization of acrylamide requires: - ANS Catalyst (APS or TEMED) or Light activation
How does agarose gel pore size relate to concentration? - ANS Inversely proportional
What is denatured during a southern blot? - ANS Hydrogen bonds (inter-strand bonds)
Melting temperature formula - ANS Tm = (4C x GC Pairs) + (2C x AT pairs)
Which is likely to interfere with fluorescent probe? - ANS 5' G
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Page
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ANSWERS
Predominant form of DNA - ANS Right-handed B
DNA and histone interaction is due to what type of bond? - ANS Ionic
What stains to the minor groove of DNA duplex? - ANS SYBR Green
What is the most abundant type of RNA? - ANS Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? - ANS Genome and mRNA
What increases half-life of mRNA? - ANS 5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? - ANS Euchromatin
What is the most common type of eukaryotic DNA? - ANS Satellite DNA
What specimen has the most DNA according to molecular weight? - ANS White Blood Cells
1
Page
What amino acids compose histones? - ANS Lysine and Arginine
@COPYRIGHT BRAINBARTER 2025/2026
, Amino acid that all proteins start with: - ANS Methionine
What protects the DNA strand during replication? - ANS Single-stranded binding protein
Leading Strand - ANS 3' to 5' template
Lagging Strand - ANS 5' to 3' template
What DNA strand is being copied in transcription? - ANS Antisense Strand
First step after newly formed mRNA is made? - ANS Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what enzyme? -
ANS Telomerase
What polymerase has repair activity? - ANS Beta/Epsilon
What polymerase acts on leading strand? - ANS Delta
What polymerase acts on lagging strand? - ANS Alpha
Which polymerase is used for mitochondrial replication and repair? - ANS Gamma
What polymerase binds the 5' end to 3' end of dsDNA? - ANS Topoisomerase
2
Page
Which RNA polymerase is mainly for transcription? - ANS RNA Pol II
@COPYRIGHT BRAINBARTER 2025/2026
, Which polymerase is involved with mRNA synthesis? - ANS RNA Pol II
What inactivates RNAses? - ANS Diethyl pyrocarbonate (DEPC) or bake glassware for 4 to 6
hours at 400C
What type of restriction enzyme recognizes palindromic sites? - ANS Restriction Enzyme
Type II
What type of restriction enzyme lacks methylation activity? - ANS Restriction Enzyme Type II
What is necessary to make cDNA? - ANS Reverse transcriptase
Number of SNPs in a human genome? - ANS 11 billion (10^7)
How many genes (base pairs) are in a human haploid genome? - ANS 3 billion base pairs
The polymerization of acrylamide requires: - ANS Catalyst (APS or TEMED) or Light activation
How does agarose gel pore size relate to concentration? - ANS Inversely proportional
What is denatured during a southern blot? - ANS Hydrogen bonds (inter-strand bonds)
Melting temperature formula - ANS Tm = (4C x GC Pairs) + (2C x AT pairs)
Which is likely to interfere with fluorescent probe? - ANS 5' G
3
Page
@COPYRIGHT BRAINBARTER 2025/2026
