ASCP EXAM REVIEW 2025/2026 Exam
Questions and Answers | A+ Score
Assured
Hgb Electrophoresis principle - 🧠ANSWER ✔✔separates hemoglobin from
blood based on mass, charge, and shape/size through electric current
Cellulose acetate agar - 🧠ANSWER ✔✔cathode (-) to anode (+)
alkaline pH: 8.4
(-)CSFA(+)
Citrate agar - 🧠ANSWER ✔✔anode (+) to cathode (-)
acidic pH: 6.2
(+)CSAF(-)
Normal Hgb electrophoresis - 🧠ANSWER ✔✔Band only at A
95% of normal Hgb is A
, A2 and F subsequently
Sickle cell trait electrophoresis - 🧠ANSWER ✔✔Equal bands at A and S
Heterozygous for sickle
Sickle cell disease electrophoresis - 🧠ANSWER ✔✔Large band at S, small
band at F
Hgb F compensating from Hgb S dysfunction
Hemoglobin C disease electrophoresis - 🧠ANSWER ✔✔Large band at C
Homozygous for Hgb C gene
Hemoglobin SC disease electrophoresis - 🧠ANSWER ✔✔Equal bands at S
and C
Heterozygous for Hgb C and S
Anti A: 4+
Anti B: 4+
A1 cells: 2+
B cells: 0
Questions and Answers | A+ Score
Assured
Hgb Electrophoresis principle - 🧠ANSWER ✔✔separates hemoglobin from
blood based on mass, charge, and shape/size through electric current
Cellulose acetate agar - 🧠ANSWER ✔✔cathode (-) to anode (+)
alkaline pH: 8.4
(-)CSFA(+)
Citrate agar - 🧠ANSWER ✔✔anode (+) to cathode (-)
acidic pH: 6.2
(+)CSAF(-)
Normal Hgb electrophoresis - 🧠ANSWER ✔✔Band only at A
95% of normal Hgb is A
, A2 and F subsequently
Sickle cell trait electrophoresis - 🧠ANSWER ✔✔Equal bands at A and S
Heterozygous for sickle
Sickle cell disease electrophoresis - 🧠ANSWER ✔✔Large band at S, small
band at F
Hgb F compensating from Hgb S dysfunction
Hemoglobin C disease electrophoresis - 🧠ANSWER ✔✔Large band at C
Homozygous for Hgb C gene
Hemoglobin SC disease electrophoresis - 🧠ANSWER ✔✔Equal bands at S
and C
Heterozygous for Hgb C and S
Anti A: 4+
Anti B: 4+
A1 cells: 2+
B cells: 0
