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BIOLOGY 1414 LAB PRACTICAL FOR 2025-2026
Biology 1414 Lab Practical
Biology 1414 Lab Practical
Biology 1414 Lab
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TABLE OF CONTENTS
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1. Important Terms and Definitions resources
2. Study Questions and Answers
3. study guide / test bank
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1. ('Why is proper disposal of biohazard waste important?', 'Prevents contamination and resources
environmental harm.')
2. ('What is the purpose of a cover slip?', 'Protects specimen and microscope lens.')
3. ('What is the purpose of a wet mount slide?', 'Observes living cells in liquid medium.')
4. ('What is the difference between a compound and dissecting microscope?', 'Compound for thin
sections, high magnification; dissecting for larger, 3D specimens at low magnification.')
STUDY QUESTIONS AND ANSWERS
Q1: What is the purpose of a standard curve in spectrophotometry?
A: To determine the concentration of unknown samples based on absorbance.
Q2: What is the function of a blank in spectrophotometry?
A: To calibrate the spectrophotometer and zero out the absorbance of the solvent.
Q3: What is the role of a micropipette tip?
A: Prevents contamination and ensures accurate liquid transfer.
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Q4: What is the difference between a positive and negative control?
A: Positive control shows the expected reaction; negative control shows no reaction.
Q5: What is the purpose of dilution?
A: Reduces the concentration of a substance to measurable levels.
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Q6: What is a serial dilution?
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A: A stepwise dilution where each solution is diluted from the previous one.
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Q7: Why is mixing important in solutions?
A: Ensures homogeneity and accurate results.
Q8: What is the purpose of a hemocytometer?
A: To count cells in a given volume of solution.
Q9: What is the function of Trypan Blue?
A: Stains dead cells to differentiate from live cells.
Q10: What is the purpose of staining a slide?
A: Enhances visibility and contrast of cellular structures.
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Study Guide - Biology 1414 Lab Practical FOR 2025-2026 - Page 4