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, a piece of skin has been stained according to a standard melanoma protocol. you
notice that after counterstaining the visible ihc product has been removed. what
most likely occurred to cause this result?
incorrect pre-treatment used
dehydrated in alcohol
incorrect antibody dilution
chromogen was outdated - correct answer-dehydrated in alcohol
in immunohistochemical staining of formalin-fixed tissue, proteolytic enzyme
pretreatment of a tissue section:
enhances background staining
unmasks antigen
epitopesis needed to demonstrate all antigens
has precise end-points - correct answer-unmasks antigen epitopes
which of the following is not a section in the msds?
ppe
physical hazards
primary routes of entry
staining procedures - correct answer-staining procedures
how many mls of a 1:5 dilution of 100% alcohol would be water?
180 ml