PORTAGE LEARNING /BIOD 171 / Microbiology Lab Notebook
Portage11Learning11B
IO11171-11Microbiology
Lab11Notebook
Lab11Notebook11Bookmarks11(click11to11naviga
te):11Lab11111Notebook
Lab11211Notebook
Lab11311Notebook
Lab11411Notebook
Lab11511Notebook
Lab11611Notebook
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Lab11911Notebook
, Lab11111Notebook
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Title:1 1 0111Introduction11to11Medical11Microbiology
Objective:
Cultivation11of11lab11samples11(growth11conditions)11that11are11necessary11for11carrying11out11exp
eriments11and11study11pathogens11in11a11lab11setting.
Procedure:
• Equipment11Used:
o Keep11a11sterile11environment11to11keep11people11healthy
o The11tool11for11this11is11the11Autoclave.
▪ Cleans11equipment11at11125⁰C
o Growing:11Fixed11incubator11or11a11shaker11incubator
▪ Fixed11incubator11operates11at113711C
▪ Shaker
▪ Visualization:11Microscopy
▪ Storage:11A11refrigerator11at11411C
o General11Lab11Safety:
▪ Never11eat11or11drink11in11the11lab
• Contamination11 risks
▪ Use11personal11protective11 equipment11(PPE)
, • Latex,11nitrile11(non-
latex11gloves)11thermal,11thermal11cold11gloves11(application
11dependent)
• Lab11coat
▪ Never11leave11the11lab11while11wearing11PPE11always11remove11before11lea
ving.
• Public11hallway
• Bathroom
• Cafeteria
o Lab11Notebook:11Required11for11the11course
▪ Titling11the11experiment:11First,11Last11initial11exp#11and11Title11of11the11ex
periment.
• Ex:11OH0111Keeping11a11Lab11Notebook
▪ Objective:11The11focus11of11the11experiment
• Ex:11Objective:11To11establish11an11organized11template11for11
keeping11experimental11records,11procedures,11and11results
.
▪ Procedure:11list11the11steps11to11any11procedure11in11the11lab.
• This11is11where11each11step11of11a11given11protocol11is11recorded.
• Be11sure11to11clearly11indicate11any11deviations11that11may11occ
ur11during11the11experiment.
▪ Notes:11Any11additional11information11placed11in11this11section.
▪ Results:11A11summary11of11the11outcome11of11the11experiment11
should11be11described11here.
Identification11of11samples11(biochemical11assays)11to11determine11what11kind11of11bacteria11or11
pathogen11a11patient11contracted.
• Tests11available11for11each11patho
gen:11Evaluation11of11samples11(microsc
opy)
• Visualization11and11recognition11of11key11characteristics11of11pathogens:
, Notes:
Equipment11Used:
o Keep11a11sterile11environment11to11keep11people11healthy
o The11tool11for11this11is11the11Autoclave.
▪ Cleans11equipment11at11125⁰C
▪ Uses11heat11pressure11and11steam11to11sterilize11your11environment.
▪ A11chamber11with11a11thick11door11that11will11be11closed11with11equ
ipment11or11a11sample11inside11to11sterilize.
▪ Uses11hot11steam11instead11of11hot11air11because11it11sterilizes11in11
a11matter11of11minutes11verses11several11hours.
▪ When11opening11the11chamber11after11a11cycle11is11complete,11be11su
re11to11open11slowly11and11carefully11after11it11has11depressurized11to1
1avoid11being11burned11by11the11hot11steam11that11will11be11released.
o Growing:11Fixed11incubator11or11a11shaker11incubator
▪ Fixed11incubator11operates11at113711C11that11houses11petri11dishes,11
which11holds11samples,11and11tubes11which11hold11liquid11cultures.11I
t11has11a11door11that11seals11such11to11maintain11dark11conditions11fo
r11growing.
▪ Shaker:11growth11media,11a11liquid11nutrient11broth,11is11placed11in11th
e11metal11holders11to11be11shook.11The11shaker11is11used11to11inoculate11
bacteria11as11it11rotates11the11sample11to11aerate11it11and11provide11opt
imal11conditions11for11bacteria11growth.
▪ Visualization:11Microscopy
▪ Storage:11A11refrigerator11at114O11C11is11used11to11slow11the11growth11of
11bacteria11and11preserve11samples.11Petri11dishes11are11wrapped11in11a
11para11film,11a11waxy11film11to11keep11it11airtight.
o General11Lab11Safety:
▪ Use11personal11protective11 equipment11(PPE)
• non-latex11available11usually11purple,11blue,11or11green
Portage11Learning11B
IO11171-11Microbiology
Lab11Notebook
Lab11Notebook11Bookmarks11(click11to11naviga
te):11Lab11111Notebook
Lab11211Notebook
Lab11311Notebook
Lab11411Notebook
Lab11511Notebook
Lab11611Notebook
Lab11711Notebook
Lab11811Notebook
Lab11911Notebook
, Lab11111Notebook
Back11to11Home11Page
Title:1 1 0111Introduction11to11Medical11Microbiology
Objective:
Cultivation11of11lab11samples11(growth11conditions)11that11are11necessary11for11carrying11out11exp
eriments11and11study11pathogens11in11a11lab11setting.
Procedure:
• Equipment11Used:
o Keep11a11sterile11environment11to11keep11people11healthy
o The11tool11for11this11is11the11Autoclave.
▪ Cleans11equipment11at11125⁰C
o Growing:11Fixed11incubator11or11a11shaker11incubator
▪ Fixed11incubator11operates11at113711C
▪ Shaker
▪ Visualization:11Microscopy
▪ Storage:11A11refrigerator11at11411C
o General11Lab11Safety:
▪ Never11eat11or11drink11in11the11lab
• Contamination11 risks
▪ Use11personal11protective11 equipment11(PPE)
, • Latex,11nitrile11(non-
latex11gloves)11thermal,11thermal11cold11gloves11(application
11dependent)
• Lab11coat
▪ Never11leave11the11lab11while11wearing11PPE11always11remove11before11lea
ving.
• Public11hallway
• Bathroom
• Cafeteria
o Lab11Notebook:11Required11for11the11course
▪ Titling11the11experiment:11First,11Last11initial11exp#11and11Title11of11the11ex
periment.
• Ex:11OH0111Keeping11a11Lab11Notebook
▪ Objective:11The11focus11of11the11experiment
• Ex:11Objective:11To11establish11an11organized11template11for11
keeping11experimental11records,11procedures,11and11results
.
▪ Procedure:11list11the11steps11to11any11procedure11in11the11lab.
• This11is11where11each11step11of11a11given11protocol11is11recorded.
• Be11sure11to11clearly11indicate11any11deviations11that11may11occ
ur11during11the11experiment.
▪ Notes:11Any11additional11information11placed11in11this11section.
▪ Results:11A11summary11of11the11outcome11of11the11experiment11
should11be11described11here.
Identification11of11samples11(biochemical11assays)11to11determine11what11kind11of11bacteria11or11
pathogen11a11patient11contracted.
• Tests11available11for11each11patho
gen:11Evaluation11of11samples11(microsc
opy)
• Visualization11and11recognition11of11key11characteristics11of11pathogens:
, Notes:
Equipment11Used:
o Keep11a11sterile11environment11to11keep11people11healthy
o The11tool11for11this11is11the11Autoclave.
▪ Cleans11equipment11at11125⁰C
▪ Uses11heat11pressure11and11steam11to11sterilize11your11environment.
▪ A11chamber11with11a11thick11door11that11will11be11closed11with11equ
ipment11or11a11sample11inside11to11sterilize.
▪ Uses11hot11steam11instead11of11hot11air11because11it11sterilizes11in11
a11matter11of11minutes11verses11several11hours.
▪ When11opening11the11chamber11after11a11cycle11is11complete,11be11su
re11to11open11slowly11and11carefully11after11it11has11depressurized11to1
1avoid11being11burned11by11the11hot11steam11that11will11be11released.
o Growing:11Fixed11incubator11or11a11shaker11incubator
▪ Fixed11incubator11operates11at113711C11that11houses11petri11dishes,11
which11holds11samples,11and11tubes11which11hold11liquid11cultures.11I
t11has11a11door11that11seals11such11to11maintain11dark11conditions11fo
r11growing.
▪ Shaker:11growth11media,11a11liquid11nutrient11broth,11is11placed11in11th
e11metal11holders11to11be11shook.11The11shaker11is11used11to11inoculate11
bacteria11as11it11rotates11the11sample11to11aerate11it11and11provide11opt
imal11conditions11for11bacteria11growth.
▪ Visualization:11Microscopy
▪ Storage:11A11refrigerator11at114O11C11is11used11to11slow11the11growth11of
11bacteria11and11preserve11samples.11Petri11dishes11are11wrapped11in11a
11para11film,11a11waxy11film11to11keep11it11airtight.
o General11Lab11Safety:
▪ Use11personal11protective11 equipment11(PPE)
• non-latex11available11usually11purple,11blue,11or11green
