BIOCHEM ACS LATEST 2025 STUDY GUIDE// Guaranteed Pass!!!

BIOCHEM ACS LATEST 2025 STUDY GUI
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EachasofastheasfollowingassubcellularasstructuresascontainsasDNAasEXCEPT

A. theasnucleus.
B. ribosomes.
C. chloroplasts.
D. mitochondria.as-asB

Inasplantasandasanimalascells,astheasthree-
dimensionalasintegrityasofastheascellasisasmaintained,asinaspart,asbyasanasint
ernalasnetworkasofasfilamentsascalled
A. theascellaswall.
B. theasGolgiasapparatus.
C. theascytoskeleton.
D. theasendoplasmicasreticulum.as-asC

Ofastheasfollowing,aswhichasareasprokaryotes?

A. viruses
B. bacteria
C. yeast
D. plantascellsas-asB

G°asforastheasconversionasofasglycerol-3-phosphateastoasglycerolas+asPiasisas-
2.2askcal/mole.asThus,asyouascanasconcludeasthat

A. thisasreactionaswillasoccurasspontaneously.
B. thisasreactionasmayasorasmayasnotasoccurasspontaneously,asdependingasona
sthe asactualasconcentrations asofasreactantsasandasproducts.

C. thisasreactionaswillasonlyasoccurasspontaneouslyasifasitasisascouple
dastoasanotherasreactionasthatashasasaasmoreasfavorableasG°.
D. thisasreactionasisassoasstronglyasfavorableasthatasitascouldasbeascouple
dastoasdriveasanasotherwiseasunfavorableascellasreaction.as-asB

Inasmostasbiochemicalasreactions,as∆PVasis
A. negligible
B. positive
C. negative
D. dependentasonas∆Eas-asA

AccordingastoastheasSecondasLawasofasThermodymnamics,

, A. theastotalasentropyaschangeasinasanyasreactionasthatasoccursasspontaneouslyasmustasbeasgr
eaterasthanas0.
B. theastotalasentropyaschangeasinasanyasreactionasthatasoccursasspontaneouslyasmustasbea
slessasthan as0.

C. theastotalasentropyasofastheasuniverseasremainsasconstant.
D. theastotalasentropyasofastheasuniverseasisasalwaysasdecreasing.as-asA

Whichasofastheasfollowingasisastrue?
A. Liquidasandassolidaswaterashaveastheassameasnumberasofashydrogenasbondsasperasmolecu
leasofaswater
B. Liquidaswaterashasasmoreashydrogenasbondsasperasmoleculeasthanasdoesassolidaswater
C. Solidaswaterashasasmoreashydrogenasbondsasperasmoleculeasthanasdoesasliquidaswater
D. Hydrogenasbondingasisasaasmajorasfactorasinastheasstructureasofasgaseousaswater
E. Noneasisastrueas-asC

WhatasisastheaspHasofasanasaqueousassolutionasthatashasasaas[OH-]asofas3.45asxas10-5asM?
A. 3.47
B. 4.47
C. 5.53
D. 9.54
E. noneasofastheasaboveas-asD

Aaspeptideaswasasfoundastoashaveasaasmolecularasmassasofasaboutas650asandasuponashydrolysisas
producedasAla,asCys,asLys,asPhe,asandasValasinasaas1:1:1:1:1asratio.asTheaspeptideasuponastreat
mentaswithasSanger'sasreagentasproducedasDNP-
Cysasandasexposureastoascarboxypeptidaseasproducedasvaline.asChymotrypsinastreatmentasofa
sthe aspeptide asproduced asaasdipeptide asthatascontainedassulfurasandashasasaasUVasabsorbance, a

sand asaastripeptide. asExposureasofasthe aspeptideastoastrypsin asproduced asaasdipeptideasandasaastri

peptide.asDeduceastheassequenceasofastheaspeptide.
A. Val-Ala-Lys-Phe-Cys
B. Cys-Lys-Phe-Ala-Val
C. Cys-Ala-Lys-Phe-Val
D. Cys-Phe-Lys-Ala-Val
E. Val-Phe-Lys-Ala-Cysas-asD

WhichasaminoasacidashasasanasR-groupaspKaasclosestastoasphysiologicalaspH?
A. lysine
B. histidine
C. glutamate
D. arginine
E. aspartateas-asB

WhichasisastrueasregardingastheasorientationasofastheasR-groupsasinasbetaasstructures?
A. InastheasparallelasbetaassheetasstructuresastheasaminoasacidasR-
groupsasareasallasonastheassameassideasofastheassheet.
B. InastheasantiparallelasbetaassheetasstructureastheasR-
groupsasonasalternateasstrandsasareasonastheassameassideasofastheassheet.

, C. InasparallelasandasantiparallelassheetsasconsecutiveasR-
groupsasonaseachaspeptideasstrandasalternateassidesasofastheassheet.
D. Becauseasofastheasfreeasrotationasaroundastheasalphaascarbon,astheasR-
groupsascanasseekastheasleastascrowdedasregionasandasareasnotasrestrictedastoasspecificasside
asofastheassheet.

E. aasandasbas-asC

Aasreagentascommonlyasemployedastoascleaveasdisulfideasbondsasinasproteinsasis
A. 2,4-dinitrofluorobenzene
B. urea
C. 2-mercaptoethanol
D. phenylisothiocyanate
E. cyanogenasbromideas-asC

Withasrespectastoasaasrelaxedasmuscle,aswhichasofastheasfollowingasisastrue?
A. theasnumberasofasactin-myosinascross-
bridgesasisasmaximizedasandastheasmuscleasisasshortened
B. theasnumberasofasactin-
myosinascrossasbridgesasisasmaximizedasandastheasmuscleasisasstretched
C. theasnumberasofasactin-
myosinascrossasbridgesasisasminimizedasandastheasmuscleasisasshortened
D. theasnumberasofasactin-
myosinascrossasbridgesasisasminimizedasandastheasmuscleasisasstretched
E. noneasofastheasaboveas-asD

Whichasofastheasfollowingasdoesasnotasdescribeashemoglobin?
A. Hemoglobinasisasanasoxygenastransportasprotein.
B. Hemoglobinasisasanasallostericasprotein.
C. Hemoglobinashasasquaternaryasstructure.
D. Copperasionsasareasimportantasinashemoglobin'sasabilityastoasbindasoxygen.
E. Hemoglobinasisascomposed,asinaspart,asofasanasironascontainingashemeasgroup.as-asD

Whichasofastheasfollowingaswouldasallowasyouastoasdetermineastheasisoelect
ricaspointasofasaasprotein?
A. proteinasquaternaryasstructure
B. proteinassolubilityasasasaasfunctionasofaspH
C. proteinassize
D. proteinasshape
E. proteinastertiaryasstructureas-asB

Aasmixtureasofasureaseas(pIas=as5.1,asmol.aswt.as482,700),ascatalaseas(pIas=as5.6asmol.aswt.as247,50
0),aslactoglobinas(pIas5.2,asmol.aswt.as37,100)asandashemoglobinas(pIas6.9,asmol.aswt.as64,500)asw
ereasappliedasinasaaspHas6.5asbufferastoasaasDEAE-
celluloseaschromatographyascolumnasandaselutedaswithastheassameasbuffer.
Whataswasastheirasorderasofaselution?
A. urease,aslactoglobin,ascatalase,ashemoglobin
B. hemoglobin,ascatalase,aslactoglobin,asurease
C. urease,ascatalase,ashemoglobin,aslactoglobin
D. lactoglobin,ashemoglobin,ascatalase,asurease
E. cannotasbeasdeterminedasfromastheasinformationasgivenas-asB