CHE 4342 Chapter 20 pt 4&5, part of 6 Questions With
Complete Solutions
7 cycles of 2 carbon additions makes how long of a FA?
Correct Answers 16
Abstract shape of Type 1 (fungi;yeast) FAS domain
organization? Correct Answers Death Star
Abstract shape of Type 1 Mammal FAS domain organization?
Correct Answers Alien space ship
Abstract shape of Type 2 (Bacteria & Plant) FAS domain
organization? Correct Answers floating globs
Acetyl-CoA cannot be used to synthesize what? Correct
Answers oxaloacetate
Attachment of headgroup to phosphatidic acid creates what?
Correct Answers Glycerophospholipid
Biosynthesis of membrane phospholipids entails what four
steps? Correct Answers Synthesis of glycerol/sphingosine
Attachment of lipids
Attachment of head groups
Some cases, alterations/exchanges of headgroups
,Citrate promotes what formation? Correct Answers filament
formation
Citrate transport mechanism across mito membrane into
cytosol? Correct Answers Citrate crosses mito membrane via
Tricarboxylate transport system,
ATP-citrate lyase forms Oxaloacetate, ejecting acyl group from
Citrate and byproducing Acetyl-SCoA
Oxaloacetate reduced via malate dehydrogenase + NADH to
Malate
Malate oxidized to pyruvate by malic enzyme with NADP+,
forming NADPH
Pyruvate crosses inner mito membrane
Pyruvate converted to oxaloacetate via pyruvate decarboxylase
Oxaloaceteate converted to citrate by adding AcetylCoA via
citrate synthase.
Dephosphorylated Acetyl-CoA carboxylase has what effect on
activity? Correct Answers increased activity
Elongation stops at how many carbons? Name of this FA?
Where are longer FAs made? What enzymes do this? Correct
Answers Elongation stops at 16 (palmitic acid).
Longer FAs are elongated in the ER by elongases.
, Epinephrine and glucagon also work on what? Correct Answers
Epinephrine and glucagon also work on adipose tissue
Evolution has done what with mammalian FAS? Correct
Answers Involved gene fusion (Type 2)
FAS in plants/bacteria uses how many enzymes? How about
ACP? Correct Answers 7, plus a separate ACP (Type 2)
FAS Step 1a? Correct Answers Acetyl-CoA primes ACP,
ejects SCoA for ACP
FAS Step 1b? Correct Answers Acetyl ACP transferred to B-
Ketoacyl Synthase
FAS Step 2? Correct Answers Malonyl CoA transferred to
Malonyl ACP, ejecting CoA for ACP
FAS Step 3? Correct Answers Condensation, ejecting CO2
making Acetoacetyl-ACP
FAS Step 4? Correct Answers First reduction by NADPH+H
to D-B-hydroxybutyryl-ACP via Ketoacyl Reductase (KR)
FAS Step 5? Correct Answers Dehydration to trans Crotonyl-
ACP via Dehydratase (DH)
FAS Step 6? Correct Answers Second reduction by
NADPH+H to Butyryl-ACP bia Enol-Reductase. Can now be
transferred back to KS to continue synthesis..
Complete Solutions
7 cycles of 2 carbon additions makes how long of a FA?
Correct Answers 16
Abstract shape of Type 1 (fungi;yeast) FAS domain
organization? Correct Answers Death Star
Abstract shape of Type 1 Mammal FAS domain organization?
Correct Answers Alien space ship
Abstract shape of Type 2 (Bacteria & Plant) FAS domain
organization? Correct Answers floating globs
Acetyl-CoA cannot be used to synthesize what? Correct
Answers oxaloacetate
Attachment of headgroup to phosphatidic acid creates what?
Correct Answers Glycerophospholipid
Biosynthesis of membrane phospholipids entails what four
steps? Correct Answers Synthesis of glycerol/sphingosine
Attachment of lipids
Attachment of head groups
Some cases, alterations/exchanges of headgroups
,Citrate promotes what formation? Correct Answers filament
formation
Citrate transport mechanism across mito membrane into
cytosol? Correct Answers Citrate crosses mito membrane via
Tricarboxylate transport system,
ATP-citrate lyase forms Oxaloacetate, ejecting acyl group from
Citrate and byproducing Acetyl-SCoA
Oxaloacetate reduced via malate dehydrogenase + NADH to
Malate
Malate oxidized to pyruvate by malic enzyme with NADP+,
forming NADPH
Pyruvate crosses inner mito membrane
Pyruvate converted to oxaloacetate via pyruvate decarboxylase
Oxaloaceteate converted to citrate by adding AcetylCoA via
citrate synthase.
Dephosphorylated Acetyl-CoA carboxylase has what effect on
activity? Correct Answers increased activity
Elongation stops at how many carbons? Name of this FA?
Where are longer FAs made? What enzymes do this? Correct
Answers Elongation stops at 16 (palmitic acid).
Longer FAs are elongated in the ER by elongases.
, Epinephrine and glucagon also work on what? Correct Answers
Epinephrine and glucagon also work on adipose tissue
Evolution has done what with mammalian FAS? Correct
Answers Involved gene fusion (Type 2)
FAS in plants/bacteria uses how many enzymes? How about
ACP? Correct Answers 7, plus a separate ACP (Type 2)
FAS Step 1a? Correct Answers Acetyl-CoA primes ACP,
ejects SCoA for ACP
FAS Step 1b? Correct Answers Acetyl ACP transferred to B-
Ketoacyl Synthase
FAS Step 2? Correct Answers Malonyl CoA transferred to
Malonyl ACP, ejecting CoA for ACP
FAS Step 3? Correct Answers Condensation, ejecting CO2
making Acetoacetyl-ACP
FAS Step 4? Correct Answers First reduction by NADPH+H
to D-B-hydroxybutyryl-ACP via Ketoacyl Reductase (KR)
FAS Step 5? Correct Answers Dehydration to trans Crotonyl-
ACP via Dehydratase (DH)
FAS Step 6? Correct Answers Second reduction by
NADPH+H to Butyryl-ACP bia Enol-Reductase. Can now be
transferred back to KS to continue synthesis..
