Baylor Genetics Lab Test 2 Questions With Complete
Solutions
Agarose gel electrophoresis Correct Answers Separates DNA
fragments based on their size
Hardy-Weinberg Equilibrium Correct Answers
How can we detect when a ddNTP is present? Correct Answers
The 4 types of ddNTPs each have a different color fluorescent
dye that can be read by a laser scanner.
How can you make a DNA stain fluoresce? Correct Answers
You can view it under ultraviolet light
How do you calculate allelic frequency? Correct Answers (#
of copies of allele in population) / 2( total # of individuals in
population)
How do you calculate genotypic frequency? Correct Answers
(# of individuals with certain phenotype) / (total # of individuals
in the population)
How do you create a recombinant plasmid? Correct Answers
The foreign DNA and the plasmid must be cut with the same
restriction enzyme so they yield sticky ends
How does natural selection limit the accumulation of changes
over time in mtDNA? Correct Answers It purges deleterious
mutations that disrupt mtDNA's vital functions.
, How does the heat shock method allow transformation to
progress? Correct Answers It creates pores in the cell's
membrane which also the ligated plasmid to enter the E. coli
cell.
How many times are the three PCR steps repeated? Correct
Answers Usually around 30 times
How much extra master mix do you want to make? Correct
Answers 10% more than the reaction calls for
Ligation reaction Correct Answers The enzyme ligase seals
the pieces of DNA to the plasmid through the creation of
phosphodiester bonds.
Multiple Cloning Site (MCS)? Correct Answers contains the
plasmid's restriction enzymes
Selectable markers Correct Answers Aid in identifying
bacterial cells that have taken up a plasmid and which of those
that are recombinant (carrying a foreign DNA insert).
What are some unique properties of mitochondrial DNA?
Correct Answers 1) It is a circular molecule
2) thousands of copies of mtDNA in a cell
3) mtDNA less prone to degradation
4) Uniparental (usually maternal) inheritance
5) Rapid mutation rate
What are the conditions for Hardy-Weinberg to apply? Correct
Answers 1) The population is infinitely large
Solutions
Agarose gel electrophoresis Correct Answers Separates DNA
fragments based on their size
Hardy-Weinberg Equilibrium Correct Answers
How can we detect when a ddNTP is present? Correct Answers
The 4 types of ddNTPs each have a different color fluorescent
dye that can be read by a laser scanner.
How can you make a DNA stain fluoresce? Correct Answers
You can view it under ultraviolet light
How do you calculate allelic frequency? Correct Answers (#
of copies of allele in population) / 2( total # of individuals in
population)
How do you calculate genotypic frequency? Correct Answers
(# of individuals with certain phenotype) / (total # of individuals
in the population)
How do you create a recombinant plasmid? Correct Answers
The foreign DNA and the plasmid must be cut with the same
restriction enzyme so they yield sticky ends
How does natural selection limit the accumulation of changes
over time in mtDNA? Correct Answers It purges deleterious
mutations that disrupt mtDNA's vital functions.
, How does the heat shock method allow transformation to
progress? Correct Answers It creates pores in the cell's
membrane which also the ligated plasmid to enter the E. coli
cell.
How many times are the three PCR steps repeated? Correct
Answers Usually around 30 times
How much extra master mix do you want to make? Correct
Answers 10% more than the reaction calls for
Ligation reaction Correct Answers The enzyme ligase seals
the pieces of DNA to the plasmid through the creation of
phosphodiester bonds.
Multiple Cloning Site (MCS)? Correct Answers contains the
plasmid's restriction enzymes
Selectable markers Correct Answers Aid in identifying
bacterial cells that have taken up a plasmid and which of those
that are recombinant (carrying a foreign DNA insert).
What are some unique properties of mitochondrial DNA?
Correct Answers 1) It is a circular molecule
2) thousands of copies of mtDNA in a cell
3) mtDNA less prone to degradation
4) Uniparental (usually maternal) inheritance
5) Rapid mutation rate
What are the conditions for Hardy-Weinberg to apply? Correct
Answers 1) The population is infinitely large
