LAB 400 - Integrated Beaker Build questions with
accurate answers
1. Extract DNA Ans✓✓✓ Add reagent to the specimen to isolate the
DNA
1. Prepare Slide Ans✓✓✓ Collect specimen and isolate the cells to fix to
a slide
2. Drop Probes Ans✓✓✓ Add fluorescent probes that are compliments to
your gene of interest
2. Polymerase Chain Reaction (PCR) Ans✓✓✓ Create many copies of
the DNA to obtain a sufficient sample size. Also called amplification
3. Barcoding/Tagging Ans✓✓✓ Attach a patient nametag at the
molecular level to the DNA strands
3. Hybridization Ans✓✓✓ Heat up the strand to allow the probe and
genome to stick if complementary
4. Library Creation Ans✓✓✓ Combine multiple patients' DNA strands
together
4. Scoring Ans✓✓✓ Two cytogenetic techs separately count the number
of colored signals which correspond to a probe-gene match under a
microscope and then compare them
,5. Ready for Review Ans✓✓✓ Cytogenetic techs interpret the meaning
of the data for the patient
5. Ready to Sequence Ans✓✓✓ Checkpoint for sequencing process to
begin. Sample goes on the instrument
6. Bioinformatic Processing Ans✓✓✓ Interface message containing raw
sequence read data is sent to a bioinformatic pipeline where patient
DNA is compared it to a known human reference sequence. Differences
are called variants, and these are interfaced back into Epic
6. Director Sign-out Ans✓✓✓ Molecular pathologist reviews and signs
out the results
7. Resulting Ans✓✓✓ Pathologists interpret the variants
8. Pathologist Sign Out Ans✓✓✓ Pathologist reviews the results and
verifies them
9. Molecular Director Review and Sign-out Ans✓✓✓ The molecular
director reviews the results and verifies them. Relevant results are sent
to the patient's chart for the provider to view
A variant is pathogentic Ans✓✓✓ If it is disease-causing
, All the chromosomes of a patient make up their Ans✓✓✓ genomes
Attaching a Rule Ans✓✓✓ - The rule's context limits where you can link
a rule
- Different rule fields require rules with different contexts
Batch Scheduler Ans✓✓✓ Includes job (E1C), batch (E1B), and run
(E1D) records which determine when the result reports are printed
Beaker Result Reports Ans✓✓✓ - Printed result reports are often
required for sending results to submitters or to affiliated physicians not
using EpicCare or EpicCare Link
- You can configure result reports based on the needs of your facility or
your submitters
Building a Rule Ans✓✓✓ - Rules are a way to build customized
conditions without needing to know how to program (condition under
which something should happen)
- Can use rules in many places in the system to allow for added
flexibility
1. Plan a Rule Build
2. Creating a Rule
3. Testing a Rule
4. Attaching a Rule
accurate answers
1. Extract DNA Ans✓✓✓ Add reagent to the specimen to isolate the
DNA
1. Prepare Slide Ans✓✓✓ Collect specimen and isolate the cells to fix to
a slide
2. Drop Probes Ans✓✓✓ Add fluorescent probes that are compliments to
your gene of interest
2. Polymerase Chain Reaction (PCR) Ans✓✓✓ Create many copies of
the DNA to obtain a sufficient sample size. Also called amplification
3. Barcoding/Tagging Ans✓✓✓ Attach a patient nametag at the
molecular level to the DNA strands
3. Hybridization Ans✓✓✓ Heat up the strand to allow the probe and
genome to stick if complementary
4. Library Creation Ans✓✓✓ Combine multiple patients' DNA strands
together
4. Scoring Ans✓✓✓ Two cytogenetic techs separately count the number
of colored signals which correspond to a probe-gene match under a
microscope and then compare them
,5. Ready for Review Ans✓✓✓ Cytogenetic techs interpret the meaning
of the data for the patient
5. Ready to Sequence Ans✓✓✓ Checkpoint for sequencing process to
begin. Sample goes on the instrument
6. Bioinformatic Processing Ans✓✓✓ Interface message containing raw
sequence read data is sent to a bioinformatic pipeline where patient
DNA is compared it to a known human reference sequence. Differences
are called variants, and these are interfaced back into Epic
6. Director Sign-out Ans✓✓✓ Molecular pathologist reviews and signs
out the results
7. Resulting Ans✓✓✓ Pathologists interpret the variants
8. Pathologist Sign Out Ans✓✓✓ Pathologist reviews the results and
verifies them
9. Molecular Director Review and Sign-out Ans✓✓✓ The molecular
director reviews the results and verifies them. Relevant results are sent
to the patient's chart for the provider to view
A variant is pathogentic Ans✓✓✓ If it is disease-causing
, All the chromosomes of a patient make up their Ans✓✓✓ genomes
Attaching a Rule Ans✓✓✓ - The rule's context limits where you can link
a rule
- Different rule fields require rules with different contexts
Batch Scheduler Ans✓✓✓ Includes job (E1C), batch (E1B), and run
(E1D) records which determine when the result reports are printed
Beaker Result Reports Ans✓✓✓ - Printed result reports are often
required for sending results to submitters or to affiliated physicians not
using EpicCare or EpicCare Link
- You can configure result reports based on the needs of your facility or
your submitters
Building a Rule Ans✓✓✓ - Rules are a way to build customized
conditions without needing to know how to program (condition under
which something should happen)
- Can use rules in many places in the system to allow for added
flexibility
1. Plan a Rule Build
2. Creating a Rule
3. Testing a Rule
4. Attaching a Rule
