Hematology Exam #1 MLS 325 UND Hem lectures 1-10
With complete solution 2025/2026
Define Hematology - Correct Answers The science dealing with the morphology of blood and
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blood forming tissues and their physiology and pathology.
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Il` State the difference in types of cells found in peripheral blood and bone marrow. - Correct
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Il` Answers The peripheral blood contains mature leukocytes, mature erythrocytes, and mature
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Il` platelets. The bone marrow contains immature precursor cells and matures cells.
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Il` Identify the 3 types of cellular elements found normally in peripheral blood - Correct Answers
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Il` Leukocytes, erythrocytes and platelets Il` Il` Il`
Il` Identify
the 6 types of white blood cells found normally in peripheral blood. - Correct Answers
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Neutrophils, band neutrophils, eosinophils, basophils, lymphocytes, and monocytes.
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Il` State the function of each cell found on a normal peripheral blood smear. - Correct Answers
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Il` Erythrocyte-transport oxygen and carbon dioxide Il` Il` Il` Il`
Platelet-blood clotting Il`
Leukocyte-immune defense Il`
Il` Identify the anticoagulant used most in hematology. - Correct Answers EDTA
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Il` Differentiate between serum and plasma. - Correct Answers Plasma is the medium of blood in
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Il` vivo. Serum is non-anticoagulated blood. Coagulation factors I, II, V, VIII, and XIII are not present in
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Il` serum.
,Il` Describe three methods of preparing peripheral blood smears. - Correct Answers Wedge smear,
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Il` coverslip, and spun smear Il` Il` Il`
Describe the characteristics of a well-prepared wedge blood smear and recognize inadequately-
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prepared smears, and how to correct them. - Correct Answers 2/3-3/4 of smear, straight/even
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feather edge, at least 2.5 cm long, margins narrower than slide, no streak, waves, clumps, or
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troughs, gradual transition from thick to thin.
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Il` Identify zone 1-4 on a peripheral blood smear and the cells that can be examined in zone 1, 2, and
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Il` 3. - Correct Answers Zone 1: platelets and WBCs
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Zone 2: RBC inclusion, platelets, and WBCs
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Zone 3: All cell types Il` Il` Il` Il`
Il` Identify which anticoagulant cannot be used to make blood smears. - Correct Answers Heparin,
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Il` because you get a bluish background. Il` Il` Il` Il` Il`
Il` Define Romanowsky stain and list two or three specific types of Romanowsky stains useful in
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Il` hematology. - Correct Answers A Romanowsky stain is used to visualize cellular components. Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
Il` There are three components: Methylene Blue, Eosin, and Azure B and oxidation products of
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Il` methylene blue. Two different types of Romanowsky stains are Wright, Wright-Giemsa Jenner.
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Il` Describe the principles of differential staining of cellular constituents by the various components
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Il` of Romanowsky dyes - Correct Answers There are three components: Methylene Blue, which
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Il` stains acidic components blue Il` Il` Il`
Eosin, which stains the basic components red
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Azure B and oxidation products of methylene blue, which occur when the stain ages.
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Il` State the color of the following cellular components with Wright/Giemsa stain: RNA,
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Il` mitochondria, Golgi, eosinophil granules, basophil granules, neutrophil granules, nucleus. - Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
, Correct Answers RNA-blue/purple Mitochondria-no stain Golgi-no stain eosinophil granule-
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reddish orange basophil granules-purple Neutrophil granules-purple Nucleus-purple
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Il` Reference interval Neutrophil - Correct Answers 1.8-7.0 x 10^9/L
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Il` Reference interval band neutrophil - Correct Answers 0-0.7 x 109/L
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Il` Reference interval lymphocyte - Correct Answers 1.0-4.8 x 10^9/L
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Il` Reference interval monocyte - Correct Answers 0.1-0.8 x 10^9/L
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Il` Reference interval eosinophil - Correct Answers 0-0.4 x 10^9/L
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Il` Reference interval Basophil - Correct Answers 0-0.2 x 10^9/L
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Il` RBC (erythrocyte) morphologic Characteristics - Correct Answers 7-8 micrometers, no nucleus,
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Il` biconcave shape, stain red because of presence of hemglobin
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Il` Platelet morphologic characteristics - Correct Answers 2-4 micrometers, no nuclei, have reddish
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Il` purple granules
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Il` Neutrophil morphologic characteristics - Correct Answers 9-15 micrometers, cytoplasm
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Il` uniformly sized pale pinkish to lavender granules (fine sand, glassy) Nucleus-dark purple color,
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Il` heavily clumped, 2-5 lobes (3-4 are normal) attached by a fine filament that has length but no
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Il` breadth.
With complete solution 2025/2026
Define Hematology - Correct Answers The science dealing with the morphology of blood and
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blood forming tissues and their physiology and pathology.
Il` Il` Il` Il` Il` Il` Il` Il`
Il` State the difference in types of cells found in peripheral blood and bone marrow. - Correct
Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
Il` Answers The peripheral blood contains mature leukocytes, mature erythrocytes, and mature
Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
Il` platelets. The bone marrow contains immature precursor cells and matures cells.
Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
Il` Identify the 3 types of cellular elements found normally in peripheral blood - Correct Answers
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Il` Leukocytes, erythrocytes and platelets Il` Il` Il`
Il` Identify
the 6 types of white blood cells found normally in peripheral blood. - Correct Answers
Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
Neutrophils, band neutrophils, eosinophils, basophils, lymphocytes, and monocytes.
Il` Il` Il` Il` Il` Il` Il` Il`
Il` State the function of each cell found on a normal peripheral blood smear. - Correct Answers
Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
Il` Erythrocyte-transport oxygen and carbon dioxide Il` Il` Il` Il`
Platelet-blood clotting Il`
Leukocyte-immune defense Il`
Il` Identify the anticoagulant used most in hematology. - Correct Answers EDTA
Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
Il` Differentiate between serum and plasma. - Correct Answers Plasma is the medium of blood in
Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
Il` vivo. Serum is non-anticoagulated blood. Coagulation factors I, II, V, VIII, and XIII are not present in
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Il` serum.
,Il` Describe three methods of preparing peripheral blood smears. - Correct Answers Wedge smear,
Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
Il` coverslip, and spun smear Il` Il` Il`
Describe the characteristics of a well-prepared wedge blood smear and recognize inadequately-
Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
prepared smears, and how to correct them. - Correct Answers 2/3-3/4 of smear, straight/even
Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
feather edge, at least 2.5 cm long, margins narrower than slide, no streak, waves, clumps, or
Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
troughs, gradual transition from thick to thin.
Il` Il` Il` Il` Il` Il` Il`
Il` Identify zone 1-4 on a peripheral blood smear and the cells that can be examined in zone 1, 2, and
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Il` 3. - Correct Answers Zone 1: platelets and WBCs
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Zone 2: RBC inclusion, platelets, and WBCs
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Zone 3: All cell types Il` Il` Il` Il`
Il` Identify which anticoagulant cannot be used to make blood smears. - Correct Answers Heparin,
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Il` because you get a bluish background. Il` Il` Il` Il` Il`
Il` Define Romanowsky stain and list two or three specific types of Romanowsky stains useful in
Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
Il` hematology. - Correct Answers A Romanowsky stain is used to visualize cellular components. Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
Il` There are three components: Methylene Blue, Eosin, and Azure B and oxidation products of
Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
Il` methylene blue. Two different types of Romanowsky stains are Wright, Wright-Giemsa Jenner.
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Il` Describe the principles of differential staining of cellular constituents by the various components
Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
Il` of Romanowsky dyes - Correct Answers There are three components: Methylene Blue, which
Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
Il` stains acidic components blue Il` Il` Il`
Eosin, which stains the basic components red
Il` Il` Il` Il` Il` Il`
Azure B and oxidation products of methylene blue, which occur when the stain ages.
Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
Il` State the color of the following cellular components with Wright/Giemsa stain: RNA,
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Il` mitochondria, Golgi, eosinophil granules, basophil granules, neutrophil granules, nucleus. - Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
, Correct Answers RNA-blue/purple Mitochondria-no stain Golgi-no stain eosinophil granule-
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reddish orange basophil granules-purple Neutrophil granules-purple Nucleus-purple
Il` Il` Il` Il` Il` Il`
Il` Reference interval Neutrophil - Correct Answers 1.8-7.0 x 10^9/L
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Il` Reference interval band neutrophil - Correct Answers 0-0.7 x 109/L
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Il` Reference interval lymphocyte - Correct Answers 1.0-4.8 x 10^9/L
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Il` Reference interval monocyte - Correct Answers 0.1-0.8 x 10^9/L
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Il` Reference interval eosinophil - Correct Answers 0-0.4 x 10^9/L
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Il` Reference interval Basophil - Correct Answers 0-0.2 x 10^9/L
Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
Il` RBC (erythrocyte) morphologic Characteristics - Correct Answers 7-8 micrometers, no nucleus,
Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
Il` biconcave shape, stain red because of presence of hemglobin
Il` Il` Il` Il` Il` Il` Il` Il`
Il` Platelet morphologic characteristics - Correct Answers 2-4 micrometers, no nuclei, have reddish
Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
Il` purple granules
Il`
Il` Neutrophil morphologic characteristics - Correct Answers 9-15 micrometers, cytoplasm
Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
Il` uniformly sized pale pinkish to lavender granules (fine sand, glassy) Nucleus-dark purple color,
Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
Il` heavily clumped, 2-5 lobes (3-4 are normal) attached by a fine filament that has length but no
Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il` Il`
Il` breadth.
