ASCP MLS QUESTIONS & ANSWERS||
2025 LATEST UPDATE!!!
minimum length of time need to save reports in micro - ANSWER 2 years
double zone of hemolysis - ANSWER Clostridium perfringens
hgb ss disease gene substitution - ANSWER a valine substituted for a glutamic acid at
the 6th position of the 13 helix of beta chain.
CML translocation - ANSWER t(9:22) BCR-ABL, philadelphia chromosome
AML translocation - ANSWER t(8,21)
APL translocation - ANSWER t(15;17)
Which chromosome causes CML - ANSWER philadelphia
How long should you fast when having blood drawn - ANSWER 12-16 hours
What percentage lower is whole blood than plasma (glucose) - ANSWER 15%
How much higher is capillary blood than venous? - ANSWER 2-5 mg/dl higher
What is clinitest used for? - ANSWER To measure inborn errors of metabolism of
glucose
Addisons disease - ANSWER hypocortisolism
Cushings - ANSWER hypercortisolism
Symptoms: obesity, moon face, slow growth in children, striae, buffalo hump, excess
hair.
Direct damage to liver cells, Gilbert's disease, Crigler Najar, Dublin Johnson, Rotor's
syndrome, cirrhosis, alcoholism, carcinoma, are all examples of what? (pre hepatic,
hepatic, or post hepatic?) - ANSWER hepatic
Hepatic results? what's increased? - ANSWER total bil, conjugated bili increased
unconjugated and urine urobilinogen normal or increased.
MCH Calculation - ANSWER Hgb x 10/RBC
MCHC calculation - ANSWER hgb x 100/HCT
, NRBC correction - ANSWER WBC x 100/NRBC count + 100
Cell count calculation - ANSWER WBC average x dil factor x 10/#squares x 1 x 0.1
ACID BASE REF RANGE - ANSWER ph - 7.35 - 7.45
CO2 - 45-35
HC03 - 22-26
Urine creatinine calculation - ANSWER urine/serum x volume/time x 1.73/BSA
Kleihauer-Betke test calculation - ANSWER # cells counted /1000
That number x 5,000
That number /30
# or Rghgm vials
What is the most common pattern of ANA - ANSWER speckled
second most common ANA - ANSWER homogenous
centromere pattern ANA - ANSWER Indicates CREST
Discrete patterns of 46 dots.
Know the light chain, heavy chain, variable region and constant region on the antibody
diagram. - ANSWER Here tis young lad behold the beauty of the diagram!
What is the half life of digoxin? - ANSWER 38 hours
What measures clumping factor coagulase? - ANSWER Slide test
What measures free coagulase? - ANSWER Tube method
What are the major biochemical characteristics of Yersinia pests? - ANSWER urease,
oxidase negative, gram negative, catalase pos.
What are the main characteristics of brucella? - ANSWER oxidase, urease, catalase
positive
nonmotile
What values can increase if delay in centrifugation? (4) - ANSWER potassium
alt
ast
creat
What major values can decrease if delay in centrifugation? (4) - ANSWER glucose,
ionized ca, bicarb, folate
2025 LATEST UPDATE!!!
minimum length of time need to save reports in micro - ANSWER 2 years
double zone of hemolysis - ANSWER Clostridium perfringens
hgb ss disease gene substitution - ANSWER a valine substituted for a glutamic acid at
the 6th position of the 13 helix of beta chain.
CML translocation - ANSWER t(9:22) BCR-ABL, philadelphia chromosome
AML translocation - ANSWER t(8,21)
APL translocation - ANSWER t(15;17)
Which chromosome causes CML - ANSWER philadelphia
How long should you fast when having blood drawn - ANSWER 12-16 hours
What percentage lower is whole blood than plasma (glucose) - ANSWER 15%
How much higher is capillary blood than venous? - ANSWER 2-5 mg/dl higher
What is clinitest used for? - ANSWER To measure inborn errors of metabolism of
glucose
Addisons disease - ANSWER hypocortisolism
Cushings - ANSWER hypercortisolism
Symptoms: obesity, moon face, slow growth in children, striae, buffalo hump, excess
hair.
Direct damage to liver cells, Gilbert's disease, Crigler Najar, Dublin Johnson, Rotor's
syndrome, cirrhosis, alcoholism, carcinoma, are all examples of what? (pre hepatic,
hepatic, or post hepatic?) - ANSWER hepatic
Hepatic results? what's increased? - ANSWER total bil, conjugated bili increased
unconjugated and urine urobilinogen normal or increased.
MCH Calculation - ANSWER Hgb x 10/RBC
MCHC calculation - ANSWER hgb x 100/HCT
, NRBC correction - ANSWER WBC x 100/NRBC count + 100
Cell count calculation - ANSWER WBC average x dil factor x 10/#squares x 1 x 0.1
ACID BASE REF RANGE - ANSWER ph - 7.35 - 7.45
CO2 - 45-35
HC03 - 22-26
Urine creatinine calculation - ANSWER urine/serum x volume/time x 1.73/BSA
Kleihauer-Betke test calculation - ANSWER # cells counted /1000
That number x 5,000
That number /30
# or Rghgm vials
What is the most common pattern of ANA - ANSWER speckled
second most common ANA - ANSWER homogenous
centromere pattern ANA - ANSWER Indicates CREST
Discrete patterns of 46 dots.
Know the light chain, heavy chain, variable region and constant region on the antibody
diagram. - ANSWER Here tis young lad behold the beauty of the diagram!
What is the half life of digoxin? - ANSWER 38 hours
What measures clumping factor coagulase? - ANSWER Slide test
What measures free coagulase? - ANSWER Tube method
What are the major biochemical characteristics of Yersinia pests? - ANSWER urease,
oxidase negative, gram negative, catalase pos.
What are the main characteristics of brucella? - ANSWER oxidase, urease, catalase
positive
nonmotile
What values can increase if delay in centrifugation? (4) - ANSWER potassium
alt
ast
creat
What major values can decrease if delay in centrifugation? (4) - ANSWER glucose,
ionized ca, bicarb, folate
