ASCP Microbiology Review – Updated Actual Exam Questions and Correct Answers (2025 Edition)

ASCP Review: Microbiology questions and answers 2024\2025 A+
Grade




Which of the following is the correct temperature at which an autoclave must be run?



-37*C

-121*C

-25*C
- correct answer 121*C



How long is an autoclave run?
- correct answer 15 mins



How many lbs per square inch of pressure is necessary when using an autoclave?
- correct answer 15 lbs/sq in



Which of the following is the correct depth and pH of a Mueller Hinton plate?



-6mm and pH 7.2-7.4

-3mm and pH 6.5-6.8

-4mm and 7.2-7.4
- correct answer 4mm and pH 7.2-7.4



What is the McFarland standard for use in the Kirby Bauer method?
- correct answer 0.5 (10^8 organisms)



Which combination of organisms are used as QC for susceptibility testing using the Kirby Bauer method?

, -S. pyogenes, P. mirabilis, N. lactamica

-P. vulgaris, S.pyogenes

-E. coli, S.aureus, P. aeruginosa
- correct answer E. coli, S.aureus, P. aeruginosa



Which anaerobic bacteria is isolated on Bacteroides Bile Esculin Agar (BBE)? What color do the colonies
appear as?



-B. fragilis, black colonies

-C. difficile, green colonies

-B. pertussis, yellow colonies
- correct answer B. fragilis, black colonies



Kanamycin-Vancomycin Laked Blood is used to isolated which anaerobic bacteria?



-Clostridium species

-Legionella species

-Bacteroides species
- correct answer Bacteroides species



Cycloserine-Cefoxitin Fructose Agar (CCFA) is used to isolate which anaerobic bacteria?



-Mycobacteria species

-C. diptheriae

-C. difficile
- correct answer C. difficile



What factors is CDC Anaerobic Blood Agar enriched with?



-hemin, cysteine, and vitamin K