Microbiology LAB Exam #1 Question and answers verified to
pass 2025
1. Microscope- The Condenser focuses the light ging into the objective lens
2. Microscope- The Iris Diaphragm adjusts the amount of light going into the
objective lens
3. Microscope- Nosepiece holds the objective lenses and rotates to change
from
one objective to another
4. Microscope- Coarse moves the stage larger distances to help get the
Objective Knob object in focus
5. Microscope- Fine moves the stage smaler increments to help get
Adjustment Knob the ob- ject in better focus
6. Microscope- Objective Lens magnifies the object. It is located near the object.
7. Microscope- Occular Lens magnifies the image of the object from the
objective
lens. It is located near the eye.
8. Steps in using the microscope 1) scanner power in place
2) place slide of specimen stage in place
with stage clips
3) Using the course adjustment knob, raise
the stage all the way up and then down slowly
until you can see image of specimen
4) Readjust with fine adjustment knob
9. Describe the purpose of the oil im- Used when oil is added to the slide. Oil
must touch the
mersion lens object and objective lens to that the light does not refract
futher
1/
29
,Microbiology LAB Exam #1 Question and answers verified to
pass 2025
10. Parfocal Parfocal microscopes are able to move from one
objec- tive to the next and still be in focus due
to the length of the objective lenses
2/
29
, Microbiology LAB Exam #1 Question and answers verified to
pass 2025
11. Resolution The ability to distinguish between two objects that
are very close together.
12. What is resolving power? The measurement of resolution
13. What are two ways to 1. By decreasing the wavelength of light
increase re- solving power? (using a blue filter) 2. Increasing the numerical
aperature
14. What units are used to express re- nm or nanometers
solving power?
15. What is better, smaller or Smaller is better
larger resolving power? Light microscopes have a 200nm resolving power
Electron microscopes have a .2nm resolving power
16. What is chromatic aberration? The ettect when many colors are seen toward the
edge
of the microscope field of vision (like a prisim)
17. How are microscopes With a special achromatic lens (corrected for red
corrected for chromatic and blue light) or by using a blue filter. The lab has
aberration? blue filters in their microscopes which also
increase resolution.
18. Describe the process of 1) Add the specimen to the slide
making a wet-mount slide 2) Touch the edge of the cover slip with one
edge of drop of specimen.
3) Let the cover slip drop gradually to the slide,
covering the drop of specimen.
19. Describe the purpose of making a To be able to see living organisms under
the microscope
3/
29
pass 2025
1. Microscope- The Condenser focuses the light ging into the objective lens
2. Microscope- The Iris Diaphragm adjusts the amount of light going into the
objective lens
3. Microscope- Nosepiece holds the objective lenses and rotates to change
from
one objective to another
4. Microscope- Coarse moves the stage larger distances to help get the
Objective Knob object in focus
5. Microscope- Fine moves the stage smaler increments to help get
Adjustment Knob the ob- ject in better focus
6. Microscope- Objective Lens magnifies the object. It is located near the object.
7. Microscope- Occular Lens magnifies the image of the object from the
objective
lens. It is located near the eye.
8. Steps in using the microscope 1) scanner power in place
2) place slide of specimen stage in place
with stage clips
3) Using the course adjustment knob, raise
the stage all the way up and then down slowly
until you can see image of specimen
4) Readjust with fine adjustment knob
9. Describe the purpose of the oil im- Used when oil is added to the slide. Oil
must touch the
mersion lens object and objective lens to that the light does not refract
futher
1/
29
,Microbiology LAB Exam #1 Question and answers verified to
pass 2025
10. Parfocal Parfocal microscopes are able to move from one
objec- tive to the next and still be in focus due
to the length of the objective lenses
2/
29
, Microbiology LAB Exam #1 Question and answers verified to
pass 2025
11. Resolution The ability to distinguish between two objects that
are very close together.
12. What is resolving power? The measurement of resolution
13. What are two ways to 1. By decreasing the wavelength of light
increase re- solving power? (using a blue filter) 2. Increasing the numerical
aperature
14. What units are used to express re- nm or nanometers
solving power?
15. What is better, smaller or Smaller is better
larger resolving power? Light microscopes have a 200nm resolving power
Electron microscopes have a .2nm resolving power
16. What is chromatic aberration? The ettect when many colors are seen toward the
edge
of the microscope field of vision (like a prisim)
17. How are microscopes With a special achromatic lens (corrected for red
corrected for chromatic and blue light) or by using a blue filter. The lab has
aberration? blue filters in their microscopes which also
increase resolution.
18. Describe the process of 1) Add the specimen to the slide
making a wet-mount slide 2) Touch the edge of the cover slip with one
edge of drop of specimen.
3) Let the cover slip drop gradually to the slide,
covering the drop of specimen.
19. Describe the purpose of making a To be able to see living organisms under
the microscope
3/
29
