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Exam (elaborations)

LAT Unit Two Exam | Verified Q&A Review on Sterilization, Sanitation, Aquatic Systems, and Biosafety

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This document delivers a focused set of verified questions and answers from the LAT Unit Two exam, covering key subjects such as biosafety protocols, sterilization methods, water quality, sanitation procedures, aquatic system management, and facility operation standards. It is ideal for Laboratory Animal Technician candidates preparing for the mid-level certification exams.

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Laboratory Animal Technician
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Uploaded on
July 7, 2025
Number of pages
7
Written in
2024/2025
Type
Exam (elaborations)
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Questions & answers

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LAT Unit Two Exam questions with verified
answers
A room will be at what type of pressure if the airflow into the room is
greater than the exhaust flow out? Ans✓✓✓ Positive


An improper light cycle and excessive noise levels have been
documented to have negative effects on what biological process of
rodents? Ans✓✓✓ Reproduction


At what biosafety level would highly infectious agents without a known
effective treatment be found? Ans✓✓✓ 4


Detergents containing this class of compound should never be used to
clean amphibian housing? Ans✓✓✓ Phenolics


Fish can develop what condition of the water becomes oversaturated
with dissolved gases? Ans✓✓✓ Gas bubble disease


HEPA filters are only efficient up to _____ of particulate matter?
Ans✓✓✓ 99.97%


How do biological indicators demonstrate ineffective sterilization?
Ans✓✓✓ The indicator organism grows in culture

, How is ammonia gas produced in soiled cage bedding? Ans✓✓✓ From
bacterial metabolism of urine products


How many general classes of biological safety cabinets are there?
Ans✓✓✓ 3


Some microorganisms survive dry heat sterilization by producing what
structure? Ans✓✓✓ Spores


What agency requires that disinfectant label claims be truthful?
Ans✓✓✓ United States Environmental Protection Agency


What aquatic species was previously used in pregnancy assays?
Ans✓✓✓ African clawed frog


What are the byproducts of ammonia breakdown? Ans✓✓✓ Nitrite and
nitrate


What can be used as a biological filter for an aquatic tank system?
Ans✓✓✓ Gravel


What criteria must be met for UV light to kill microorganisms?
Ans✓✓✓ Water must flow at the proper rate


What do individual ventilated cages control? Ans✓✓✓ Air quality

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