BCH 330 Exam 1 questions with answers

BCH 330 Exam 1 questions with answers|\ |\ |\ |\ |\ |\




rules of conjugate acid to conjugate base - CORRECT ANSWERS
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✔✔when pH = pKa, ca/cb = 1, pH=pKa-1, ca/cb = 0.9/0.1,
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pH=pKa +1, ca/cb=0.1/0.9 |\ |\




pH = pKa + log [base]/[acid] - CORRECT ANSWERS
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✔✔Henderson-Hasselbalch equation |\




Relate pKa to Pyruvate in the equation - CORRECT ANSWERS
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✔✔Ka = [pyruvate][H+]/[pyruvic acid]
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relate pKa to Ka - CORRECT ANSWERS ✔✔pKa = -logKa
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What does pKa do - CORRECT ANSWERS ✔✔pKa determines the
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affinity of a proton for the functional group it binds to
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When pH<pKa, the affinity of H+ favors _______________ side of
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the equilibrium - CORRECT ANSWERS ✔✔The pyruvic acid
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What is the practical significance of pKa - CORRECT ANSWERS
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✔✔It allows us to determine how readily the proton reacts
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R - CORRECT ANSWERS ✔✔8.314 mol-1 K-1
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,dG = -RTlnK - CORRECT ANSWERS ✔✔J/mol
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dG = dH - TdS - CORRECT ANSWERS ✔✔Gibbs equation
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What can Gibbs free energy be used for? - CORRECT ANSWERS
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✔✔to determine if folding or unfolding is favored
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- dG is (folding or unfolding) - CORRECT ANSWERS ✔✔unfolding
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+ dG is (folding or unfolding) - CORRECT ANSWERS ✔✔folding
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If dG = 0, folding:unfolding is - CORRECT ANSWERS ✔✔in
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equilibrium


Enthalpy (energy) - CORRECT ANSWERS ✔✔the embedded
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energy in a hydrogen bond |\ |\ |\ |\




higher enthalpy favors folding because_____ - CORRECT
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ANSWERS ✔✔hydrogen bonds are higher energy |\ |\ |\ |\ |\




Entropy - CORRECT ANSWERS ✔✔difference in configurations
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Entropy increases as the protein ______ - CORRECT ANSWERS
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✔✔unfolds

, Since S is affected by temperature, higher temperatures favor
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_____ contribution, which favors _____________ - CORRECT
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ANSWERS ✔✔entropic, denaturation |\ |\




Between 10 and 50, folding is favored because ________________ -
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CORRECT ANSWERS ✔✔enthalpic contribution allows for greater
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hydrogen bonding energy since a higher or closer network of
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hydrogen bonding is formed |\ |\ |\




What allows for stabilization due to the hydrophobic effect -
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CORRECT ANSWERS ✔✔Enthalpic contribution outweighs the
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entropic contribution |\




sigmoidal denaturation curve - CORRECT ANSWERS ✔✔S shaped
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curve, x=Temperature, y=absorbance
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pH dependence curve - CORRECT ANSWERS ✔✔x = pH, y =
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number of protons removed|\ |\ |\




Biological significance of protein denaturation - CORRECT
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ANSWERS ✔✔At temperatures above 60C, the protein denatures
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and it can't function properly anymore
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The hydrophobic effect - CORRECT ANSWERS ✔✔hydrophobic
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proteins on the inside and hydrophilic proteins on the outside
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