Biochem Final Exam Questions With 100% Correct And Verified Answers 2025

Biochem Final Exam Questions
With 100% Correct And Verified
Answers 2025
Fateiofipyruvateiunderihypoxiciorianaerobiciconditionsiandi
whatiorganismi-iCORRECTiANSWERS-
2iEthanoli+i2iCO2i(yeast)iorilactatei(vigorouslyicontractingi
muscle,iredibloodicellsiandisomeiothericells/organisms)

Fateiofipyruvateiunderiaerobiciconditionsiandiwhatiorganis
msi-iCORRECTiANSWERS-Acetyl-
CoA,igoesithroughicitriciacidicycleitoiformi4CO2iandi4H2Oi(
animals,iplants,iandimicrobialicells)

OxidativeistepsiofiPPPi-iCORRECTiANSWERS-
makeiNADPH,ifavorediwhenitheicellineedsiNADPH

Non-oxidativeistepsiofiPPPi-iCORRECTiANSWERS-
makeiribose,ifavorediwhenitheicellineedsinucleotides

Modei1iofiPPPi-iCORRECTiANSWERS--
iOccursiinirapidlyidividingicellsibecauseitheyineedilotsiofinuc

leotidesiforiDNA
-iskipsioxidativeisteps
-icostsiATP

Modei2iofiPPPi-iCORRECTiANSWERS--
iBalancedineediforiboth

-imainlyioxidative
-ibothiproductsiproducedi(NADPHiandiRibosei5-phosphate)
-inoiglycolysisineeded

Modei3iofiPPPi-iCORRECTiANSWERS--
icellineedsilotsiofiNADPH

-
icellsithatidoianabolismirequireiNADPHitoipreventifreeiradic

alidamage


vendarsol

,-iMaximumiNADPHicreated
-iribosei5-
phosphateiconvertedibackitoiG6Piviaigluconeogenesis

Modei4iofiPPPi-iCORRECTiANSWERS--
iATPiandiNADPHineeded

-iribose-5-
phosphateiconverteditoipyruvateiviaiglycolysisiiniorderitoipr
oduceimoreiATP

aerobicicellularirespirationi-iCORRECTiANSWERS--
icellsiconsumeiO2iandiproduceiCO2

-iprovidesimoreiATPifromiglucoseithaniglycolysis
-iusedibyianimals,iplants,iandimicroorganisms

Stagesioficellularirespirationi-iCORRECTiANSWERS--iacetyl-
CoAiproduction
-acetyl-CoAioxidationi(viaiCAC)
-ielectronitransferiandioxidativeiphosphorylation

Stagei1ioficellularirespirationi-iCORRECTiANSWERS-
aminoiacids,ifattyiacids,iglucoseialliconverteditoiAcetyl-CoA

Stagei2ioficellularirespirationi-iCORRECTiANSWERS--iAcety-
CoAiisioxidized
-iNADH,iFADH2iareiproduced

Stagei3ioficellularirespirationi-iCORRECTiANSWERS--
iNADH,iFADH2ireleaseielectronsitoihelpicreateiATPiinitheiET

C
-iLOTSiofiATPicreated

whereidoesiglycolysisitakeiplacei-iCORRECTiANSWERS-
cytoplasm

whereidoesiCACioccuri-iCORRECTiANSWERS-
mitochondrialimatrixi(exceptisuccinateidehydrogenase,iwhi
chiisiinitheiinnerimembrane)



vendarsol

,Whereidoesioxidativeiphosphorylationioccuri-
iCORRECTiANSWERS-innerimembrane



howiisitheicitriciacidicycleistartedi-iCORRECTiANSWERS-
pyruvateiisitransferrediintoimitochondrialimatrixibyipyruvat
eitranslocase

TypesiofivitaminiBirequirediforiCitriciAcidiCyclei-
iCORRECTiANSWERS-

Riboflavini(B2),iNiacini(B3),iThiaminei(B1),iPantotheniciaci
di(B5)

Riboflavini-iCORRECTiANSWERS--iB2
-
iprecursoriforiFAD,iwhichiisiinipyruvateiandisuccinateidehyd

rogenases

Niacini-iCORRECTiANSWERS--iB3
-iPrecursoriforiNADH/NAD+,iwhichiisipresentiinipyruvate,ia-
ketoglutarateiandimalateidehydrogenases

Thiaminei-iCORRECTiANSWERS--iB1
-iprostheticigroupiinipyruvateiandia-
ketoglutarateidehydrogenases

Pantotheniciacidi-iCORRECTiANSWERS--iB5
-iprecursoriforiCoenzymeiAi(partiofiAcetyl-CoA

ConversioniofipyruvateiintoiAcetyl-CoAi(PDC)i-
iCORRECTiANSWERS--

icatalyzedibyiE1,iE2,iandiE3iofipyruvateidehydrogenaseicomp

lexi(PDC)
-iirreversible,ihighlyiregulated
-irequiresi5icoenzymes
-iprostheticigroups:iTPP,ilipoyllysine,iFAD
-icofactorsiareiNAD+iandiCoA-SH

Reactioni1iofiCACi(citrateisynthase)i-iCORRECTiANSWERS--
iirreversibleiandirate-limiting




vendarsol

, -iacetyl-CoAiandioxaloacetateicreateicitrate
-itransferaseienzyme
-ireactionioccursiviaiacid-baseicatalysis

mechanismioficitrateisynthaseispecificallyi-
iCORRECTiANSWERS--

ioxaloacetateibindsifirst,itheniinducesifitiforiacetyl-CoA

-iCoAicleavedioffiforilotsiofienergy
-irate-
limitingistepiforiwholeiCAC,idependsioniconcentrationiofioxa
loacetate

Reactioni2aiandi2biofiCACi-iCORRECTiANSWERS--
icitrateiisidehydratediandirehydratediintoiisocitrate

-
isameienzymeiusediforibothistepsi(aconitase),iwhichiisiailyas

eitypeienzyme
-ireversible

Reactioni3iofiCACi-iCORRECTiANSWERS--
iisocitrateioxidizediintoioxalosuccinateibyihydrideitransferit

oiNAD+i(alcoholibecomesiketone)
-ioxalosuccinateidecarboxylatediandienolatediintoia-
ketoglutarate
-ioxidoreductaseitype
-iCO2iproduced,isoiproductihasioneilessicarbon
-iirreversible,iregulatedibyiproductiinhibitioniandiATP

Reactioni4iofiCACi(a-
ketoglutarateidehydrogenaseicomplex)i-
iCORRECTiANSWERS--ia-

ketoglutarateigoesithroughioxidativeidecarboxylationiintois
uccinyl-CoA
-ienzymeiisia-ketoglutarateidehydrogenaseicomplex
-ioxidoreductaseienzyme
-iirreversible




vendarsol