1
CHEM 210 Biochemistry Module 1 to 8
Exams' & Final Exam (2025/2026)
Portage Learning Questions and Verified
Answers, 100% Guarantee Pass
Module 1: The Amazing World of Biomolecules
1. What is the primary source of energy in glycolysis?
a) Pyruvate
b) Glucose
c) ATP
d) NADH
Rationale: Glucose is the initial substrate broken down in glycolysis to produce energy,
per Portage Learning curriculum.
2. Which element is considered the “superglue” of the chemical world in biomolecules?
a) Hydrogen
b) Carbon
c) Nitrogen
d) Oxygen
Rationale: Carbon’s ability to form covalent bonds and long chains makes it essential for
biomolecules, per Portage Learning Module 1.
3. True or False: A compound with a molecular mass of 1,000 g/mol is considered a
macromolecule.
a) True
b) False
c) Depends on the molecule
d) Only for proteins
Rationale: Macromolecules typically have a molecular mass >5,000 g/mol, per Portage
Learning standards.
4. Which functional group is found in all carbohydrates?
a) Amine
b) Hydroxyl
c) Phosphate
d) Carboxyl
Rationale: Carbohydrates contain hydroxyl (-OH) groups, essential for their structure,
per Module 1.
5. What is the role of lysosomes in eukaryotic cells?
a) Energy production
b) Digesting macromolecules
, 2
c) Protein synthesis
d) Lipid storage
Rationale: Lysosomes degrade macromolecules, per Portage Learning Module 1.
6. Which of the following is NOT a property of carbon in biomolecules?
a) Forming covalent bonds
b) Forming ionic bonds
c) Triple bond formation
d) Polymer chain formation
Rationale: Carbon primarily forms covalent bonds in biomolecules, not ionic bonds, per
Module 1.
7. What is the approximate size of a human cell?
a) 1 mm
b) 12 µm
c) 100 nm
d) 1 cm
Rationale: Human cells average ~12 µm in diameter, per Portage Learning standards.
8. Which molecule is the most important for energy storage in cells?
a) Glucose
b) ATP
c) NADH
d) FADH2
Rationale: ATP is the primary energy currency in cells, per Module 1.
9. What type of bond characterizes a Kekulé structure?
a) Ionic
b) Covalent
c) Hydrogen
d) Van der Waals
Rationale: Kekulé structures show covalent bonds between atoms, per Portage Learning
Module 1.
10. Which element is NOT typically found in living organisms?
a) Hydrogen
b) Bromine
c) Sulfur
d) Nitrogen
Rationale: Bromine is not commonly found in biomolecules, unlike H, S, and N, per
Module 1.
Module 2: Aqueous Solutions: Water and Buffers
11. What contributes to the bent shape of a water molecule?
a) Equal electron sharing
b) Unshared electron pairs on oxygen
c) Covalent bonds with hydrogen
d) Linear geometry
Rationale: Unshared electron pairs on oxygen cause water’s bent shape, per Module 2.
, 3
12. What is the pH of a solution with [H⁺] = 7.9 x 10⁻¹³ M?
a) 6.70
b) 12.1
c) -12.1
d) 11.17
Rationale: pH = -log[7.9 x 10⁻¹³] ≈ 12.1, per Module 2 calculations.
13. Which property of water is critical for biochemical reactions?
a) Low polarity
b) Hydrogen bonding
c) High density
d) Nonpolar nature
Rationale: Hydrogen bonding enables water’s role as a solvent in biochemical reactions,
per Module 2.
14. What does the Henderson-Hasselbalch equation calculate?
a) Enzyme kinetics
b) pH of a buffer
c) Gibbs free energy
d) Metabolic flux
Rationale: The Henderson-Hasselbalch equation determines buffer pH, per Portage
Learning Module 2.
15. What is the pKa of a weak acid in a buffer system?
a) The pH of the solution
b) The pH at which [HA] = [A⁻]
c) The concentration of H⁺ ions
d) The enzyme’s Km value
Rationale: pKa is the pH where the acid and its conjugate base are equal, per Module 2.
Module 3: Amino Acids, Proteins, and Enzymes
16. What is the primary structure of a protein?
a) Alpha helix
b) Amino acid sequence
c) Beta sheet
d) Quaternary assembly
Rationale: The primary structure is the linear sequence of amino acids, per Module 3.
17. Which amino acid contains a sulfur group?
a) Glycine
b) Cysteine
c) Alanine
d) Valine
Rationale: Cysteine contains a thiol (-SH) group, per Portage Learning Module 3.
18. What is a cofactor in enzyme function?
a) A substrate
b) A molecule necessary for enzyme activity
c) A product of the reaction
CHEM 210 Biochemistry Module 1 to 8
Exams' & Final Exam (2025/2026)
Portage Learning Questions and Verified
Answers, 100% Guarantee Pass
Module 1: The Amazing World of Biomolecules
1. What is the primary source of energy in glycolysis?
a) Pyruvate
b) Glucose
c) ATP
d) NADH
Rationale: Glucose is the initial substrate broken down in glycolysis to produce energy,
per Portage Learning curriculum.
2. Which element is considered the “superglue” of the chemical world in biomolecules?
a) Hydrogen
b) Carbon
c) Nitrogen
d) Oxygen
Rationale: Carbon’s ability to form covalent bonds and long chains makes it essential for
biomolecules, per Portage Learning Module 1.
3. True or False: A compound with a molecular mass of 1,000 g/mol is considered a
macromolecule.
a) True
b) False
c) Depends on the molecule
d) Only for proteins
Rationale: Macromolecules typically have a molecular mass >5,000 g/mol, per Portage
Learning standards.
4. Which functional group is found in all carbohydrates?
a) Amine
b) Hydroxyl
c) Phosphate
d) Carboxyl
Rationale: Carbohydrates contain hydroxyl (-OH) groups, essential for their structure,
per Module 1.
5. What is the role of lysosomes in eukaryotic cells?
a) Energy production
b) Digesting macromolecules
, 2
c) Protein synthesis
d) Lipid storage
Rationale: Lysosomes degrade macromolecules, per Portage Learning Module 1.
6. Which of the following is NOT a property of carbon in biomolecules?
a) Forming covalent bonds
b) Forming ionic bonds
c) Triple bond formation
d) Polymer chain formation
Rationale: Carbon primarily forms covalent bonds in biomolecules, not ionic bonds, per
Module 1.
7. What is the approximate size of a human cell?
a) 1 mm
b) 12 µm
c) 100 nm
d) 1 cm
Rationale: Human cells average ~12 µm in diameter, per Portage Learning standards.
8. Which molecule is the most important for energy storage in cells?
a) Glucose
b) ATP
c) NADH
d) FADH2
Rationale: ATP is the primary energy currency in cells, per Module 1.
9. What type of bond characterizes a Kekulé structure?
a) Ionic
b) Covalent
c) Hydrogen
d) Van der Waals
Rationale: Kekulé structures show covalent bonds between atoms, per Portage Learning
Module 1.
10. Which element is NOT typically found in living organisms?
a) Hydrogen
b) Bromine
c) Sulfur
d) Nitrogen
Rationale: Bromine is not commonly found in biomolecules, unlike H, S, and N, per
Module 1.
Module 2: Aqueous Solutions: Water and Buffers
11. What contributes to the bent shape of a water molecule?
a) Equal electron sharing
b) Unshared electron pairs on oxygen
c) Covalent bonds with hydrogen
d) Linear geometry
Rationale: Unshared electron pairs on oxygen cause water’s bent shape, per Module 2.
, 3
12. What is the pH of a solution with [H⁺] = 7.9 x 10⁻¹³ M?
a) 6.70
b) 12.1
c) -12.1
d) 11.17
Rationale: pH = -log[7.9 x 10⁻¹³] ≈ 12.1, per Module 2 calculations.
13. Which property of water is critical for biochemical reactions?
a) Low polarity
b) Hydrogen bonding
c) High density
d) Nonpolar nature
Rationale: Hydrogen bonding enables water’s role as a solvent in biochemical reactions,
per Module 2.
14. What does the Henderson-Hasselbalch equation calculate?
a) Enzyme kinetics
b) pH of a buffer
c) Gibbs free energy
d) Metabolic flux
Rationale: The Henderson-Hasselbalch equation determines buffer pH, per Portage
Learning Module 2.
15. What is the pKa of a weak acid in a buffer system?
a) The pH of the solution
b) The pH at which [HA] = [A⁻]
c) The concentration of H⁺ ions
d) The enzyme’s Km value
Rationale: pKa is the pH where the acid and its conjugate base are equal, per Module 2.
Module 3: Amino Acids, Proteins, and Enzymes
16. What is the primary structure of a protein?
a) Alpha helix
b) Amino acid sequence
c) Beta sheet
d) Quaternary assembly
Rationale: The primary structure is the linear sequence of amino acids, per Module 3.
17. Which amino acid contains a sulfur group?
a) Glycine
b) Cysteine
c) Alanine
d) Valine
Rationale: Cysteine contains a thiol (-SH) group, per Portage Learning Module 3.
18. What is a cofactor in enzyme function?
a) A substrate
b) A molecule necessary for enzyme activity
c) A product of the reaction
