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1. What is the primary purpose of a tourniquet in phlebotomy?
To make veins more visible and easier to access
A tourniquet restricts venous blood flow, causing veins to fill and become
more prominent for puncture.
2. Which vein is most commonly used for venipuncture?
Median cubital vein
This vein is large, easily accessible, and has the lowest risk of
complications.
3. What angle should the needle be inserted when performing a
venipuncture?
15 to 30 degrees
This range allows entry into the vein without passing through it.
4. What is the correct order of draw for blood collection tubes?
Blood culture, light blue, red, gold/tiger top, green, lavender, gray
This order prevents cross-contamination of additives.
,5. Which additive is found in lavender-top tubes?
EDTA
EDTA prevents clotting by binding calcium.
6. When should a phlebotomist label the specimen tubes?
Immediately after collection at the patient’s bedside
Labeling in the presence of the patient ensures accuracy and reduces
errors.
7. What is the recommended antiseptic for routine venipuncture?
70% isopropyl alcohol
This effectively disinfects the skin and reduces infection risk.
8. Which patient position is safest for blood collection?
Seated with arm extended and supported
This minimizes the risk of fainting or injury during the draw.
9. What should a phlebotomist do if a patient feels faint?
Stop the procedure immediately and lower the patient’s head
This prevents injury and helps restore blood flow to the brain.
10.Which test requires a chain of custody form?
Drug screening
Chain of custody ensures the sample’s integrity for legal purposes.
11.Which specimen should be protected from light?
Bilirubin
Light degrades bilirubin, leading to inaccurate results.
, 12.What is hemoconcentration?
An increase in the ratio of cells to plasma
It occurs if a tourniquet is left on too long.
13.What is the maximum recommended time a tourniquet should be applied?
1 minute
Prolonged application can cause hemoconcentration and inaccurate
results.
14.Why is the basilic vein the last choice for venipuncture?
It is close to arteries and nerves
This increases the risk of complications if punctured.
15.What is the first step in any blood collection procedure?
Identify the patient
Proper identification prevents specimen errors.
16.What action should be taken if a hematoma develops during venipuncture?
Remove the needle and apply pressure
This prevents further leakage of blood into the tissue.
17.Which color tube is used for a PT/INR test?
Light blue
It contains sodium citrate for coagulation studies.
18.Which type of needle is best for small or fragile veins?
Butterfly needle
Winged infusion sets allow better control and positioning.