Microscopy Exam Questions with Complete Solutions

Microscopy Exam Questions with
Complete Solutions

Magnification - ANSWERSHow much times larger the image is compared to the actual size of the
object (always refer to equation)



Resolution - ANSWERSThe ability to see different objects as separate entities



What are the 4 sample preparations - ANSWERS-dry mount

-wet mount

-squash slide

-smear slide



Explain dry mount and wet mount sample preparation - ANSWERS-dry mount: samples can be
viewed whole cut or cut into thin slices using a blade (sectioning) Specimen is placed onto a
slide with specimen

-wet mount: samples are suspended in liquid or immersion oil, coverslip is placed onto
specimen at an 45 degree angle



explain squash slide and smear slide sample preparation - ANSWERS-squash slide: a wet mount
is first prepared, a lens tissue is used to press down coverslip (may cause damage to specimen)

-smear slide: edge of coverslip is used to smear sample creating a thin even coating , coverslip is
then places



Why do we use stains in sample preparation and what is the main function of it. - ANSWERSdue
to illumination of light cells tend to have low contrast as they dont absorb light. Resolution is
limited by the wavelength of light as well as diffraction of light