BIO 351 Exam
BIO 351 - Final Exam 1 (80 Questions) With
Correct Answers New Update
1. You have made a transgene expressing a LacZ reporter gene. Where will this transgene integrate
into the genome? - CORRECT ANSWER ✔ - A random chromosomal location
2. You are able to fluorescently label cells in the ectoderm of a chick embryo and watch the
embryo over the course of a few days. You notice that the labeled cells now have developed into
cells shaped like... What best describes the process you have observed? - CORRECT ANSWER ✔ -
differentiation
3. Which of the following properties of ES cells make them useful for making knock out mutations?
- CORRECT ANSWER ✔ - they are pluripotent, will self-renew, and will integrate vector DNA by
homologous recombination
4. Which of the following is the correct order of developmental steps leading to a specialized cell
type? - CORRECT ANSWER ✔ - specification, determination, differentiation
5. The diagram below shows an example of juxtacrine signaling. Cell-cell contact is mediated
through the ____ ligand on the inducing cell and ___ receptor o the responding cell in this
pathway. - CORRECT ANSWER ✔ - Delta, Notch
6. Which of the following techniques would be most appropriate for directly studying the
secretion of Wnt1 signaling and its distribution throughout the extracellular space of the
embryonic spinal cord? - CORRECT ANSWER ✔ - In situ hybridization OR
immunohistochemistry
7. Which statement is true? - CORRECT ANSWER ✔ - Bindin in necessary for sea urchin sperm to
recognize eggs in a species-specific manner.
1|P A G E
, BIO 351 Exam
8. Differential gene expression is a central theme in development. which of the following
statements most accurately describes this concept? - CORRECT ANSWER ✔ - genomic DNA is
the same in all cell types, but mRNAs and proteins are different
9. Which of the following statements regarding conditional gene knockouts is false? - CORRECT
ANSWER ✔ - the targeting vector for inserting loxp sites utilizes nonhomologous recombination
TRUE:
-Cre/loxp can be used to delete genetic material
-Cre expression is often under the control of a cell-specific promoter
-Cre recombinase is one of many known site-specific recombinases
10. As cells move along the continuum from stem cell to differentiated cell which of the following
correctly identifies the properties of a determined cell? - CORRECT ANSWER ✔ - can
differentiate in a non-neutral environment and its fate is irreversible
11. Which of the following statements is false? - CORRECT ANSWER ✔ - Surrounding cells must
provide a cue for specifying a particular cell fate.
TRUE:
-Cells are specified before they are determined
-Specification is a reversible step in the commitment of a cell to a particular cell fate
-Cell fates are irreversible after determination
12. In order to generate a mutant rodent strain with a heritable genetic modification it is
particularly important that the ____ cell lineage possess the desire mutation. - CORRECT
ANSWER ✔ - Germ
13. ____ is an important regulator of transcription downstream of Shh signaling. - CORRECT
ANSWER ✔ - Gli
14. Reverse transcription polymerase chain reaction (RT-PCR) detects what type of molecule? -
CORRECT ANSWER ✔ - mRNA
2|P A G E
BIO 351 - Final Exam 1 (80 Questions) With
Correct Answers New Update
1. You have made a transgene expressing a LacZ reporter gene. Where will this transgene integrate
into the genome? - CORRECT ANSWER ✔ - A random chromosomal location
2. You are able to fluorescently label cells in the ectoderm of a chick embryo and watch the
embryo over the course of a few days. You notice that the labeled cells now have developed into
cells shaped like... What best describes the process you have observed? - CORRECT ANSWER ✔ -
differentiation
3. Which of the following properties of ES cells make them useful for making knock out mutations?
- CORRECT ANSWER ✔ - they are pluripotent, will self-renew, and will integrate vector DNA by
homologous recombination
4. Which of the following is the correct order of developmental steps leading to a specialized cell
type? - CORRECT ANSWER ✔ - specification, determination, differentiation
5. The diagram below shows an example of juxtacrine signaling. Cell-cell contact is mediated
through the ____ ligand on the inducing cell and ___ receptor o the responding cell in this
pathway. - CORRECT ANSWER ✔ - Delta, Notch
6. Which of the following techniques would be most appropriate for directly studying the
secretion of Wnt1 signaling and its distribution throughout the extracellular space of the
embryonic spinal cord? - CORRECT ANSWER ✔ - In situ hybridization OR
immunohistochemistry
7. Which statement is true? - CORRECT ANSWER ✔ - Bindin in necessary for sea urchin sperm to
recognize eggs in a species-specific manner.
1|P A G E
, BIO 351 Exam
8. Differential gene expression is a central theme in development. which of the following
statements most accurately describes this concept? - CORRECT ANSWER ✔ - genomic DNA is
the same in all cell types, but mRNAs and proteins are different
9. Which of the following statements regarding conditional gene knockouts is false? - CORRECT
ANSWER ✔ - the targeting vector for inserting loxp sites utilizes nonhomologous recombination
TRUE:
-Cre/loxp can be used to delete genetic material
-Cre expression is often under the control of a cell-specific promoter
-Cre recombinase is one of many known site-specific recombinases
10. As cells move along the continuum from stem cell to differentiated cell which of the following
correctly identifies the properties of a determined cell? - CORRECT ANSWER ✔ - can
differentiate in a non-neutral environment and its fate is irreversible
11. Which of the following statements is false? - CORRECT ANSWER ✔ - Surrounding cells must
provide a cue for specifying a particular cell fate.
TRUE:
-Cells are specified before they are determined
-Specification is a reversible step in the commitment of a cell to a particular cell fate
-Cell fates are irreversible after determination
12. In order to generate a mutant rodent strain with a heritable genetic modification it is
particularly important that the ____ cell lineage possess the desire mutation. - CORRECT
ANSWER ✔ - Germ
13. ____ is an important regulator of transcription downstream of Shh signaling. - CORRECT
ANSWER ✔ - Gli
14. Reverse transcription polymerase chain reaction (RT-PCR) detects what type of molecule? -
CORRECT ANSWER ✔ - mRNA
2|P A G E
