BCH 330 FINAL EXAM NEWEST 2025 TESTING ACTUAL
EXAM WITH 180 QUESTIONS AND CORRECT DETAILED
ANSWERS (VERIFIED ANSWERS) ALREADY GRADED A+
(BRAND NEW) GUARANTEED PASS
A protein known as contains 3 AMINO ACIDS that undergo an
oxidative reaction to form an intense fluorophore. This protein is
remarkably useful because the fluorophore is . Importantly, GFP can be
attached to form a with a second protein of interest during standard cloning
procedures. β-barrel, GFP, intrinsic, encoded in the amino acid sequence, fusion
protein
Bacteria move in a mode. They sense that the concentration of a nutrient
is increasing and in one direction. When they do not sense an increasing
gradient, the direction of flagellar rotation reverse. Since the flagellar fibers are
not meant to go in that direction, the bacterium . roll-and-tumble
mode, rotate their flagellum, tumbles in a random-walk fashion.
is a support medium that has two functions: 1) prevents molecules from
diffusing into broad bands 2) provides a sieving material that obstructs the
movement of larger particles more than smaller ones, allowing them to separate.
The term refers to a specific gel layer technique that "focuses" a protein
band. The mechanism involves creating a large voltage gradient between Cl- and
the in a later called the , which has a reduced pH.
Polyacrylamide, discontinuous gel, glycinate zwitterion, stacking gel
,The following equation is the analytical version of the principle of
the rate of a chemical reaction is directly proportional to the product of the
activities or concentrations of the reactants
∆G= ∆G₀ + RTlnQ, where Q=[C][D]/[A][B] mass action
What emphasizes the relative contributions of enthalpic and entropic energies to
the stabilization of globular proteins? hydrophobic effect
What does ∆G stand for? standard state free energy
What is calculated by the following equation?
K = [products] / [reactants] equilibrium constant
H = U + PV
Describes the energy absorbed/released during the making/breaking of bonds.
Enthalpy (∆H)
∆H describes the energy associated with . bond breakage/dissociation
,The term ∆S refers to , which is defined as . entropy, number of
available configurations
are dependent only on the total concentration of a solute species.
Examples include pressure lowering, boiling point elevation, freezing point
depression, and osmotic pressure. colligative properties
is the phenomena by which H₂O tries to dilute the counterions that are
bound to the macromolecule. This makes the concentration of H₂O higher near
the molecule than in the bulk solution. This results in the property of in
which a charged protein or nucleic acid contains an electrostricted ion-H₂O shell.
Donan effect, electrostriction
colligative properties may also be related
defines the concentration dependence of ∆G. One example is that it
drives osmotic pressure.
μ = ∆G/∆n = ∆G/(∆cV) chemical potenial
definitions and their equations are used to describe a derivative when
one or more other variables are held constant partial differentials
∆U = q + w
, where ∆U refers to the of a , as contributed to by (q) and
work (w). The amount of energy it takes to go from one state to another.
ΔU = TΔS + uΔn - PΔV internal energy, system, heat
( ) has the dimensions of energy and can be used to calculate
using the following equation:
¹/₂ KbT, where Kb = 1.381x10^-23 JK^-1 Boltzman's contant (Kb), degrees of
freedom
Q = Σj cj = Σj exp (-ΔGj/RT) describes , which is the ratio of concentrations
of a compound in a mixture of two immiscible phases at equilibrium. This ratio is
therefore a measure of the difference in solubility of the compound in these two
phases.
Example of this is dissolving benzene in H₂O (Csw) vs. oil (Cso) or the difference
between an AA presence in water or benzene to determine its
hydrophobicity/philicity partition coefficient
d ln Keq / d (1/T) = -ΔH°/R
ln Keq = -∆H/RT + ∆S/R
EXAM WITH 180 QUESTIONS AND CORRECT DETAILED
ANSWERS (VERIFIED ANSWERS) ALREADY GRADED A+
(BRAND NEW) GUARANTEED PASS
A protein known as contains 3 AMINO ACIDS that undergo an
oxidative reaction to form an intense fluorophore. This protein is
remarkably useful because the fluorophore is . Importantly, GFP can be
attached to form a with a second protein of interest during standard cloning
procedures. β-barrel, GFP, intrinsic, encoded in the amino acid sequence, fusion
protein
Bacteria move in a mode. They sense that the concentration of a nutrient
is increasing and in one direction. When they do not sense an increasing
gradient, the direction of flagellar rotation reverse. Since the flagellar fibers are
not meant to go in that direction, the bacterium . roll-and-tumble
mode, rotate their flagellum, tumbles in a random-walk fashion.
is a support medium that has two functions: 1) prevents molecules from
diffusing into broad bands 2) provides a sieving material that obstructs the
movement of larger particles more than smaller ones, allowing them to separate.
The term refers to a specific gel layer technique that "focuses" a protein
band. The mechanism involves creating a large voltage gradient between Cl- and
the in a later called the , which has a reduced pH.
Polyacrylamide, discontinuous gel, glycinate zwitterion, stacking gel
,The following equation is the analytical version of the principle of
the rate of a chemical reaction is directly proportional to the product of the
activities or concentrations of the reactants
∆G= ∆G₀ + RTlnQ, where Q=[C][D]/[A][B] mass action
What emphasizes the relative contributions of enthalpic and entropic energies to
the stabilization of globular proteins? hydrophobic effect
What does ∆G stand for? standard state free energy
What is calculated by the following equation?
K = [products] / [reactants] equilibrium constant
H = U + PV
Describes the energy absorbed/released during the making/breaking of bonds.
Enthalpy (∆H)
∆H describes the energy associated with . bond breakage/dissociation
,The term ∆S refers to , which is defined as . entropy, number of
available configurations
are dependent only on the total concentration of a solute species.
Examples include pressure lowering, boiling point elevation, freezing point
depression, and osmotic pressure. colligative properties
is the phenomena by which H₂O tries to dilute the counterions that are
bound to the macromolecule. This makes the concentration of H₂O higher near
the molecule than in the bulk solution. This results in the property of in
which a charged protein or nucleic acid contains an electrostricted ion-H₂O shell.
Donan effect, electrostriction
colligative properties may also be related
defines the concentration dependence of ∆G. One example is that it
drives osmotic pressure.
μ = ∆G/∆n = ∆G/(∆cV) chemical potenial
definitions and their equations are used to describe a derivative when
one or more other variables are held constant partial differentials
∆U = q + w
, where ∆U refers to the of a , as contributed to by (q) and
work (w). The amount of energy it takes to go from one state to another.
ΔU = TΔS + uΔn - PΔV internal energy, system, heat
( ) has the dimensions of energy and can be used to calculate
using the following equation:
¹/₂ KbT, where Kb = 1.381x10^-23 JK^-1 Boltzman's contant (Kb), degrees of
freedom
Q = Σj cj = Σj exp (-ΔGj/RT) describes , which is the ratio of concentrations
of a compound in a mixture of two immiscible phases at equilibrium. This ratio is
therefore a measure of the difference in solubility of the compound in these two
phases.
Example of this is dissolving benzene in H₂O (Csw) vs. oil (Cso) or the difference
between an AA presence in water or benzene to determine its
hydrophobicity/philicity partition coefficient
d ln Keq / d (1/T) = -ΔH°/R
ln Keq = -∆H/RT + ∆S/R
