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Samenvatting Tabel Identificatiemedia (niet-selectieve voedingsbodems)

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Deze uitgebreide tabel bevat een complete lijst van alle niet-selectieve voedingsbodems (identificatiemedia) en al hun eigenschappen voor het vak Microbiologie 2 (afstudeerrichting Chemie). Docent: P. Devoldere

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Uploaded on
September 24, 2020
Number of pages
4
Written in
2018/2019
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Summary

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Voedingsbodem Substraat Indicator Entwijze Incubati Mogelijk Toe te Beoordeling Verklaring van het
(vast, halfvast of (substraten) (indicatoren) e- uitzicht na voegen uitzicht na incubatie
vloeibaar) omstandi incubatie reagentia
(in buisje of g-heden na
petriplaat) incubatie

Tryptonwater = Tryptofaan Kovacs Öse 37 C Donkerrode ring Kovacs Rode ring : Indool + (C. Perfringens)
Indooltest Reagens Geelbruine ring : Indool -
 vloeibaar
Motility Agar = Steekentin 37 C Verspreiding Beweeglijk
beweeglijkheids- g langs de Onbeweeglijk C. Perfringens :
test steekenting onbeweeglijk
 half-vast (uitzwerming)
SBG = Gelatine Steek 22 C Vloeibaar (+) Gelatinase + Eiwit gelatine wordt
gelatinase-test (anders Vast omgezet tot gelatinase
 Standaard smelt Gelatinase -
Bouillon Gelatine gelatine) C. Perfringens :
 vast, buisje Gelatinase +
Gelatinasetest Gelatine Lijnenting 37 C Witte neerslag HgCl2 Gelatinase + Gelatine vormt met
volgens Clarcke zware metalen een witte
 Standaard Heldere zone Gelatinase - neerslag (-)
Bouillon Gelatine rond entlijn + C. Perfringens :
 vast, plaat Gelatinase +
Gelatinasetest Gelatine Houtskool- Suspensie 37 C Tablet intact Gelatinase + Gelatinase breekt het
volgens Kohn- gelatineschijfje Tablet tabletje af
Lautrop afgebroken Gelatinase - C. Perfringens :
 vloeibaar (susp) (zwart) Gelatinase +
Standaard KNO3 Omgekeerd Öse 37 C Rood Griesreagen NO3-  NO2- Meteen rood = red. +
nitraat bouillon= durhambuisje s A en B NO3-  N2 Niet rood na Zn =
nitraatreductase Niet rood Of Zn reductse +
-test NO3-  NO3- Rood na Zn = red. –
 vloeibaar C. Perfringens : red. +
Nutriënt Boullion Lactose 1% Durhambuisje Öse 37 C Geel Broomthym Zuur
met Lactose = Groenblauw olblauw Basisch C. Perfringens
lactosevergisting Gasbel Met gasvorming Lact + & gasvorming
 vloeibaar
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