PRE-MEDICAL
ZOOLOGY
ENTHUSIAST | LEADER | ACHIEVER
EXERCISE
Biotechnology : Principles & Processes
and Applications
ENGLISH MEDIUM
,Biology : Biotechnology ALLEN®
Pre-Medical
EXERCISE-I (Conceptual Questions) Build Up Your Understanding
PRINCIPLES AND PROCESSES 8. When the genotype of an organism is
1. Agrobacterium tumefaciens used in Genetic improved by the addition of foreign gene,
engineering for :- the process is called
(1) DNA-mapping (1) Tissue culture
(2) DNA-modification (2) Genetic diversity
(3) Gene transfer (3) Genetic engineering
(4) DNA finger printing (4) Plastic surgery
BT0001 BT0009
2. Who isolated the first restriction 9. A genetically manipulated organism
endonucleases :- containing in its genome one or more
(1) Temin & Baltimore (2) Sanger inserted gene of another species is called :-
(3) Smith (4) Paul berg (1) Transposon
BT0003 (2) Gene expression
3. Genetic engineering is :- (3) Transgenic organism
(1) Study of extra nuclear gene (4) Retroposons
(2) Manipulation of genotype by artificial
BT0010
method
10. Which vector is commonly used in the
(3) Manipulation of RNA
transfer of gene in a crop plant -
(4) Manipulation of enzymes
(1) Plasmids of B. Subtilis
BT0004
(2) Bacteriophages
4. Polymerase chain reaction technology
(3) Ti-plasmids of Agrobacterium
(PCR-technique) is used for :-
(1) DNA identification (4) E. Coli Phages
(2) DNA repair BT0011
(3) DNA amplification 11. The tumour indusing capacity of
(4) Cleave DNA Agrobacterium tumaefaciens is located in
BT0005 extrachromosomal plasmid and called–
5. Which structure involved in genetic (1) Ti – plasmid
engineering:- (2) Ri - plamid
(1) Plastid (2) Plasmid (3) Lambda phage
(3) Codon (4) None (4) Plasmid PBR 322
BT0006 BT0012
6. Which of the following is the example of 12. Genetic engineering aims at :-
chemical scissors – (1) Destroying wild gene
(1) ECo - RI (2) Hind - III (2) Preserving defective gene
(3) Bam - I (4) All the above (3) Curing human disease by introducing
BT0007 new gene
7. Restriction endonucleases are used in (4) All the above
genetic engineering bacause :- BT0013
(1) They can degrade harmful proteins 13. A piece of nucleic acid using to find out a
(2) They can join DNA fragments gene, by forming hybrid with it, is called
(3) They can cut DNA at variable site as:-
(4) They can cut DNA at specific base (1) c – DNA (2) DNA probe
sequences (3) Sticky end (4) Blunt end
BT0014
BT0008
33
,ALLEN® Biology : Biotechnology
Pre-Medical
14. Taq - polymerase which is used for 20. Restriction endonucleases :-
amplification of DNA related with :- (1) Are synthesized by bacteria as part of
(1) Hybridoma technique their defense mechanism
(2) PCR-technique (2) Are present in mammalian cells for
(3) Gene cloning degradation of DNA when the cell dies
(4) r-DNA technology (3) Are used in genetic engineering for
BT0015 ligating two DNA molecules
15. What is true for plasmid :– (4) Are used for invitro DNA synthesis
(1) Plasmids are widely used in gene
BT0021
transfer
(2) These are found in virus 21. The Ti plasmid, is often used for making
(3) Plasmid contains gene for vital activities transgenic plants. This plasmid is found in :-
(4) These are main part of chromosome (1) Yeast
BT0016 (2) Azotobacter
16. Which of the following cuts the DNA from (3) Rhizobium
specific places :– (4) Agrobacterium
(1) Restriction endonuclease BT0022
(2) Ligase 22. Which of the following is the example of
(3) Exonuclease direct gene transfer :-
(4) Alkaline phosphate (1) Microinjection
BT0017 (2) Electroporation
17. Manipulation of DNA in genetic
(3) Particle gun
engineering became possible due to the
discovery of :– (4) All the above
(1) Restriction endonuclease BT0023
(2) DNA ligase 23.. How many copies of DNA sample are
(3) Transcriptase produced in PCR technique after 6–cycle:-
(4) Primase (1) 4 (2) 32 (3) 64 (4) 16
BT0018 BT0024
18. Which one of the following has found 24. Thermal cycle takes place in which
extensive use in genetic engineering work technique
in plants (1) Gel electrophoresis
(1) Bacillus coagulens (2) PCR-technique
(2) Agrobacterium tumefaciens.
(3) Centrifugation
(3) Clostridium septicum
(4) Southern blotting
(4) Xanthomonas citri
BT0019 BT0025
19. Restriction endonucleases : 25. BAC and YAC are :-
(1) Are enzymes which cleave DNA at (1) Natural DNA obtained from bacteria
specific sites and yeast
(2) Make DNA complementary to an (2) Useful vectors for eukaryotic gene
existing DNA or RNA transfer
(3) Cut or join DNA fragments (3) Artificial DNA obtained from bacteria
(4) Are required in vectorless direct gene and yeast
transfer. (4) (2) & (3) both
BT0020 BT0027
34
, Biology : Biotechnology ALLEN®
Pre-Medical
26. Restriction enzymes are :– 30. The restriction enzyem ECORI has the
(1) Not always required in genetic property of
engineering (1) endonuclease activity
(2) Essential tool in genetic engineering (2) exonuclease activity
(3) Nucleases that cleave DNA at specific (3) ligation activity
sites (4) correcting the topology of replicating
(4) (2) and (3) both DNA
BT0028 BT0033
27. Electroporation procedure involves : 31. DNA ligase is an enzyme that catalyses
(1) Fast passage of food through sieve the:-
pores in phloem elements with the help (1) splitting of DNA threads into small bits
of electric stimulation. (2) joining of the fragments of DNA
(2) Opening of stomatal pores during night
(3) denaturation of DNA
by artificial light
(4) synthesis of DNA
(3) Making transient pores in the cell
BT0034
membrane to introduce gene
constructs 32. Agrobacterium tumefaciens contains a
(4) Purification of saline water with the large plasmid, which induces tumour in the
help of a membrane system. plants it is termed as -
BT0030 (1) Ti plasmid
28. Which of the following restriction (2) Ri plasmid
endonuclease enzyme produce blunt end in (3) Recombinant plasmid
DNA :- (4) Shine Delgrano sequence
BT0035
(1) Bam HI G G A T C C 33. More advancement in genetic engineering
CC TAGG is due to :-
(1) Restriction endonuclease
(2) Reverse transcriptase
(2) ECORI G A A T T C (3) Protease
C TTAAG (4) Zymase
BT0036
34. Which of the follwing is used as a best
(3) Hae–III G G C C
genetic vector in plants :-
CCGG
(1) Bacillus thuriengenesis
(2) Agrobacterium tumifaciens
(4) All the above
(3) Pseudomonas putida
BT0031
(4) All of these
29. A bacterium modifies its DNA by adding
methyl groups to the DNA, It does so to :- BT0037
(1) Clone its DNA 35. Which of the following enzyme is used to
(2) Be able to transcribe many genes join DNA fragments :-
simultaneously (1) Terminase
(3) Turn its gene on (2) Endonuclease
(4) Protect its DNA from its own restriction (3) Ligase
enzyme (4) DNA polymerase
BT0032 BT0038
35
ZOOLOGY
ENTHUSIAST | LEADER | ACHIEVER
EXERCISE
Biotechnology : Principles & Processes
and Applications
ENGLISH MEDIUM
,Biology : Biotechnology ALLEN®
Pre-Medical
EXERCISE-I (Conceptual Questions) Build Up Your Understanding
PRINCIPLES AND PROCESSES 8. When the genotype of an organism is
1. Agrobacterium tumefaciens used in Genetic improved by the addition of foreign gene,
engineering for :- the process is called
(1) DNA-mapping (1) Tissue culture
(2) DNA-modification (2) Genetic diversity
(3) Gene transfer (3) Genetic engineering
(4) DNA finger printing (4) Plastic surgery
BT0001 BT0009
2. Who isolated the first restriction 9. A genetically manipulated organism
endonucleases :- containing in its genome one or more
(1) Temin & Baltimore (2) Sanger inserted gene of another species is called :-
(3) Smith (4) Paul berg (1) Transposon
BT0003 (2) Gene expression
3. Genetic engineering is :- (3) Transgenic organism
(1) Study of extra nuclear gene (4) Retroposons
(2) Manipulation of genotype by artificial
BT0010
method
10. Which vector is commonly used in the
(3) Manipulation of RNA
transfer of gene in a crop plant -
(4) Manipulation of enzymes
(1) Plasmids of B. Subtilis
BT0004
(2) Bacteriophages
4. Polymerase chain reaction technology
(3) Ti-plasmids of Agrobacterium
(PCR-technique) is used for :-
(1) DNA identification (4) E. Coli Phages
(2) DNA repair BT0011
(3) DNA amplification 11. The tumour indusing capacity of
(4) Cleave DNA Agrobacterium tumaefaciens is located in
BT0005 extrachromosomal plasmid and called–
5. Which structure involved in genetic (1) Ti – plasmid
engineering:- (2) Ri - plamid
(1) Plastid (2) Plasmid (3) Lambda phage
(3) Codon (4) None (4) Plasmid PBR 322
BT0006 BT0012
6. Which of the following is the example of 12. Genetic engineering aims at :-
chemical scissors – (1) Destroying wild gene
(1) ECo - RI (2) Hind - III (2) Preserving defective gene
(3) Bam - I (4) All the above (3) Curing human disease by introducing
BT0007 new gene
7. Restriction endonucleases are used in (4) All the above
genetic engineering bacause :- BT0013
(1) They can degrade harmful proteins 13. A piece of nucleic acid using to find out a
(2) They can join DNA fragments gene, by forming hybrid with it, is called
(3) They can cut DNA at variable site as:-
(4) They can cut DNA at specific base (1) c – DNA (2) DNA probe
sequences (3) Sticky end (4) Blunt end
BT0014
BT0008
33
,ALLEN® Biology : Biotechnology
Pre-Medical
14. Taq - polymerase which is used for 20. Restriction endonucleases :-
amplification of DNA related with :- (1) Are synthesized by bacteria as part of
(1) Hybridoma technique their defense mechanism
(2) PCR-technique (2) Are present in mammalian cells for
(3) Gene cloning degradation of DNA when the cell dies
(4) r-DNA technology (3) Are used in genetic engineering for
BT0015 ligating two DNA molecules
15. What is true for plasmid :– (4) Are used for invitro DNA synthesis
(1) Plasmids are widely used in gene
BT0021
transfer
(2) These are found in virus 21. The Ti plasmid, is often used for making
(3) Plasmid contains gene for vital activities transgenic plants. This plasmid is found in :-
(4) These are main part of chromosome (1) Yeast
BT0016 (2) Azotobacter
16. Which of the following cuts the DNA from (3) Rhizobium
specific places :– (4) Agrobacterium
(1) Restriction endonuclease BT0022
(2) Ligase 22. Which of the following is the example of
(3) Exonuclease direct gene transfer :-
(4) Alkaline phosphate (1) Microinjection
BT0017 (2) Electroporation
17. Manipulation of DNA in genetic
(3) Particle gun
engineering became possible due to the
discovery of :– (4) All the above
(1) Restriction endonuclease BT0023
(2) DNA ligase 23.. How many copies of DNA sample are
(3) Transcriptase produced in PCR technique after 6–cycle:-
(4) Primase (1) 4 (2) 32 (3) 64 (4) 16
BT0018 BT0024
18. Which one of the following has found 24. Thermal cycle takes place in which
extensive use in genetic engineering work technique
in plants (1) Gel electrophoresis
(1) Bacillus coagulens (2) PCR-technique
(2) Agrobacterium tumefaciens.
(3) Centrifugation
(3) Clostridium septicum
(4) Southern blotting
(4) Xanthomonas citri
BT0019 BT0025
19. Restriction endonucleases : 25. BAC and YAC are :-
(1) Are enzymes which cleave DNA at (1) Natural DNA obtained from bacteria
specific sites and yeast
(2) Make DNA complementary to an (2) Useful vectors for eukaryotic gene
existing DNA or RNA transfer
(3) Cut or join DNA fragments (3) Artificial DNA obtained from bacteria
(4) Are required in vectorless direct gene and yeast
transfer. (4) (2) & (3) both
BT0020 BT0027
34
, Biology : Biotechnology ALLEN®
Pre-Medical
26. Restriction enzymes are :– 30. The restriction enzyem ECORI has the
(1) Not always required in genetic property of
engineering (1) endonuclease activity
(2) Essential tool in genetic engineering (2) exonuclease activity
(3) Nucleases that cleave DNA at specific (3) ligation activity
sites (4) correcting the topology of replicating
(4) (2) and (3) both DNA
BT0028 BT0033
27. Electroporation procedure involves : 31. DNA ligase is an enzyme that catalyses
(1) Fast passage of food through sieve the:-
pores in phloem elements with the help (1) splitting of DNA threads into small bits
of electric stimulation. (2) joining of the fragments of DNA
(2) Opening of stomatal pores during night
(3) denaturation of DNA
by artificial light
(4) synthesis of DNA
(3) Making transient pores in the cell
BT0034
membrane to introduce gene
constructs 32. Agrobacterium tumefaciens contains a
(4) Purification of saline water with the large plasmid, which induces tumour in the
help of a membrane system. plants it is termed as -
BT0030 (1) Ti plasmid
28. Which of the following restriction (2) Ri plasmid
endonuclease enzyme produce blunt end in (3) Recombinant plasmid
DNA :- (4) Shine Delgrano sequence
BT0035
(1) Bam HI G G A T C C 33. More advancement in genetic engineering
CC TAGG is due to :-
(1) Restriction endonuclease
(2) Reverse transcriptase
(2) ECORI G A A T T C (3) Protease
C TTAAG (4) Zymase
BT0036
34. Which of the follwing is used as a best
(3) Hae–III G G C C
genetic vector in plants :-
CCGG
(1) Bacillus thuriengenesis
(2) Agrobacterium tumifaciens
(4) All the above
(3) Pseudomonas putida
BT0031
(4) All of these
29. A bacterium modifies its DNA by adding
methyl groups to the DNA, It does so to :- BT0037
(1) Clone its DNA 35. Which of the following enzyme is used to
(2) Be able to transcribe many genes join DNA fragments :-
simultaneously (1) Terminase
(3) Turn its gene on (2) Endonuclease
(4) Protect its DNA from its own restriction (3) Ligase
enzyme (4) DNA polymerase
BT0032 BT0038
35
