BIOL 410 Final
Study online at https://quizlet.com/_h4h6vt
1. Which of the following pipettes would you P-200
use to measure 150 μL?
2. What is molecular biology? the study of life at a molecular level (DNA,
RNA, Proteins, Lipids, polysaccharides)
3. What is the main focus of molecular biolo- The study of molecular structures and their
gy? functions within living organisms.
4. What is a major advantage of mastering It enhances skills in experimental design,
molecular biology techniques (MolTech)? problem-solving, and data analysis
5. What type of water is recommended for Ultrapure water
making laboratory solutions?
6. Why should you avoid letting the pipette It can lead to inaccurate measurements and
plunger snap back after aspirating liquid? may damage the pistons, potentially caus-
ing rust.
Both D and C
7. Why is EDTA included in TE buffer? To bind metal ions that are necessary for
DNase function
8. What role does ethanol play in the DNA ex- It helps precipitate DNA from the solution
traction process?
9. What is hyperchromicity? An increase in absorbance as DNA dena-
tures
10. Which of the following increases the melt- Higher salt concentration
ing temperature of DNA?
11. Which challenge in DNA extraction can Polysaccharide interference
CTAB address?
, BIOL 410 Final
Study online at https://quizlet.com/_h4h6vt
12. What is the role of chaotropic salts in sili- Bind DNA to silica
ca-based DNA extraction?
13. What does the Central Dogma of molecular The flow of genetic information within a cell
biology explain?
14. Which DNA extraction method is most ef- Phenol-chloroform extraction
fective for samples with protein contami-
nation?
15. What type of bond connects nucleotides in Phosphodiester bonds
a DNA strand?
16. What stabilizes DNA by stacking interac- Van der Waals forces
tions?
17. Which of the following is a component of a Phosphate group
nucleotide but NOT a nucleoside?
18. What does "in vitro" refer to? Outside a living organism, e.g., in a test
tube
19. Which type of gel electrophoresis is typical- Sodium dodecyl-sulfate polyacrylamide gel
ly used for proteins? electrophoresis (SDS-PAGE)
20. In gel electrophoresis, how does DNA move Toward the anode
through the gel?
21. What does the size of the DNA fragment The speed at which it moves through the gel
affect in gel electrophoresis?
22. Which of the following is a major advantage It amplifies DNA quickly, often in hours
of PCR?
Study online at https://quizlet.com/_h4h6vt
1. Which of the following pipettes would you P-200
use to measure 150 μL?
2. What is molecular biology? the study of life at a molecular level (DNA,
RNA, Proteins, Lipids, polysaccharides)
3. What is the main focus of molecular biolo- The study of molecular structures and their
gy? functions within living organisms.
4. What is a major advantage of mastering It enhances skills in experimental design,
molecular biology techniques (MolTech)? problem-solving, and data analysis
5. What type of water is recommended for Ultrapure water
making laboratory solutions?
6. Why should you avoid letting the pipette It can lead to inaccurate measurements and
plunger snap back after aspirating liquid? may damage the pistons, potentially caus-
ing rust.
Both D and C
7. Why is EDTA included in TE buffer? To bind metal ions that are necessary for
DNase function
8. What role does ethanol play in the DNA ex- It helps precipitate DNA from the solution
traction process?
9. What is hyperchromicity? An increase in absorbance as DNA dena-
tures
10. Which of the following increases the melt- Higher salt concentration
ing temperature of DNA?
11. Which challenge in DNA extraction can Polysaccharide interference
CTAB address?
, BIOL 410 Final
Study online at https://quizlet.com/_h4h6vt
12. What is the role of chaotropic salts in sili- Bind DNA to silica
ca-based DNA extraction?
13. What does the Central Dogma of molecular The flow of genetic information within a cell
biology explain?
14. Which DNA extraction method is most ef- Phenol-chloroform extraction
fective for samples with protein contami-
nation?
15. What type of bond connects nucleotides in Phosphodiester bonds
a DNA strand?
16. What stabilizes DNA by stacking interac- Van der Waals forces
tions?
17. Which of the following is a component of a Phosphate group
nucleotide but NOT a nucleoside?
18. What does "in vitro" refer to? Outside a living organism, e.g., in a test
tube
19. Which type of gel electrophoresis is typical- Sodium dodecyl-sulfate polyacrylamide gel
ly used for proteins? electrophoresis (SDS-PAGE)
20. In gel electrophoresis, how does DNA move Toward the anode
through the gel?
21. What does the size of the DNA fragment The speed at which it moves through the gel
affect in gel electrophoresis?
22. Which of the following is a major advantage It amplifies DNA quickly, often in hours
of PCR?
