Heme lab practical 2 MLT to MLS UAMS
UPDATED ACTUAL Exam Questions and
CORRECT Answers
Pros about automated counting - CORRECT ANSWER - Speed
Accuracy
Eliminates subjectivity
Precision
More cells are counted
Small blood volume
Pros about Manuel counting - CORRECT ANSWER - Low Cost
Good for low work volume
Fewer artifacts and spurious results
Impedance - CORRECT ANSWER - Detects and measures changes in electrical resistance
between two electrodes as cells pass through a sensing aperture
How is the cell population evaluated on an automated cell counter? - CORRECT
ANSWER - Cell population is evaluated based on size
Light Scatter - CORRECT ANSWER - Blood is hydro dynamically focused by sheathing
fluid which moves cells forward the center of the column
As laser is interrupted, cells are counted and sized
In WBC Histograms, 35-90fL is what? - CORRECT ANSWER - Lymphs
In WBC Histograms, 90-160fL is what? - CORRECT ANSWER - Mononuclear Cells:
, Monocytes, Blasts, Immature Granulocytes, reactive lymphs
In WBC Histograms,160-450fL is what? - CORRECT ANSWER - Granulocytes
In a RBC Histogram, what amount is counted as RBCs? (in fl) - CORRECT ANSWER -
>36 fL
In a Platelet histogram, particles between ____ and ____ are considered in the count. -
CORRECT ANSWER - 2-20 fL
How much does an automated counter draw up? - CORRECT ANSWER - 100 microliters
What are the two that go into the RBC bath dilution in an automated counter? - CORRECT
ANSWER - the RBCs and the Plts
What are the two that go into the WBC bath dilution in an automated counter? - CORRECT
ANSWER - WBC and Hgb
Directly measured parameters of WBCs - CORRECT ANSWER - Impedence
All cells >35 fL are counted
Cells are categorized by pulse height
Cytoplasm is collapsed around nucleus to differentiate lymph, mono, granulocyte
Directly measured parameters of RBCs - CORRECT ANSWER - Impedence
Cells >36 fL are counted
WBC are included in this count!!
Directly measured parameters of platelets - CORRECT ANSWER - Impedence
Counted in same bath as RBC
UPDATED ACTUAL Exam Questions and
CORRECT Answers
Pros about automated counting - CORRECT ANSWER - Speed
Accuracy
Eliminates subjectivity
Precision
More cells are counted
Small blood volume
Pros about Manuel counting - CORRECT ANSWER - Low Cost
Good for low work volume
Fewer artifacts and spurious results
Impedance - CORRECT ANSWER - Detects and measures changes in electrical resistance
between two electrodes as cells pass through a sensing aperture
How is the cell population evaluated on an automated cell counter? - CORRECT
ANSWER - Cell population is evaluated based on size
Light Scatter - CORRECT ANSWER - Blood is hydro dynamically focused by sheathing
fluid which moves cells forward the center of the column
As laser is interrupted, cells are counted and sized
In WBC Histograms, 35-90fL is what? - CORRECT ANSWER - Lymphs
In WBC Histograms, 90-160fL is what? - CORRECT ANSWER - Mononuclear Cells:
, Monocytes, Blasts, Immature Granulocytes, reactive lymphs
In WBC Histograms,160-450fL is what? - CORRECT ANSWER - Granulocytes
In a RBC Histogram, what amount is counted as RBCs? (in fl) - CORRECT ANSWER -
>36 fL
In a Platelet histogram, particles between ____ and ____ are considered in the count. -
CORRECT ANSWER - 2-20 fL
How much does an automated counter draw up? - CORRECT ANSWER - 100 microliters
What are the two that go into the RBC bath dilution in an automated counter? - CORRECT
ANSWER - the RBCs and the Plts
What are the two that go into the WBC bath dilution in an automated counter? - CORRECT
ANSWER - WBC and Hgb
Directly measured parameters of WBCs - CORRECT ANSWER - Impedence
All cells >35 fL are counted
Cells are categorized by pulse height
Cytoplasm is collapsed around nucleus to differentiate lymph, mono, granulocyte
Directly measured parameters of RBCs - CORRECT ANSWER - Impedence
Cells >36 fL are counted
WBC are included in this count!!
Directly measured parameters of platelets - CORRECT ANSWER - Impedence
Counted in same bath as RBC
