Instrumental analysis exam 3 questions with
verified answers
absorption chromatography (liquid-solid) Ans✓✓✓ -used to separate
relatively non polar species with MW<5000
-a particular strength of this method is that it resolves isometric
mixtures such a meta and para benzene derivatives much easier than
other techniques
adjusted retention time Ans✓✓✓ -(t'R)X = (t'R)X-TM
-where (t'r)x is the retention time of the analyte X and TM is the
retention time of an unretained species (mobile phase).
-adjusted retention time is the difference between the retention time
of the analyte minus the dead time. Adjusted retention time refers to
the amount of time the analyte spends interacting with the stationary
phase.
affinity chromatography Ans✓✓✓ -affinity chromatography can be
used in a number of applications, including nucleic acid purification,
protein purification from cell free extracts, and purification from blood.
by using affinity chromatography, one can separate proteins that bind a
certain fragment from proteins that do not bind that specific fragment.
-1) Inject a sample into an initially equilibrated affinity chromatography
column
, -2) only the substances with affinity for the ligand are retained in the
column.
-3) other substances with no affinity for the ligand are eluted from the
column.
bubble cell Ans✓✓✓ -if bubbles form in the mobile phase bottle, it
indicates the mobile phase has become supersaturated with dissolved
air.
-all steps in column chromatography procedures depend on efficient
and uniform contact of solution with the solid support. If air bubbles
form in the resin bed, they limit contact with the resin and impede flow
through the column.
capillary zone electrophoresis Ans✓✓✓ -capillary zone electrophoresis
(CZE) is a technique successfully used for the separation of proteins,
peptides, and nucleic acids. Other applications where CZE may be
useful include analysis of inorganic anions and cations, such as those
typically separated by ion chromatography.
chromatogram Ans✓✓✓ -a time-based graphic record (as a
concentration of eluted materials) of a chromatographic separation.
-as the compounds come out of the column, we measure the intensity
of those peaks. The detector response is a function of time, so we have
a record of when each compound is coming out.
verified answers
absorption chromatography (liquid-solid) Ans✓✓✓ -used to separate
relatively non polar species with MW<5000
-a particular strength of this method is that it resolves isometric
mixtures such a meta and para benzene derivatives much easier than
other techniques
adjusted retention time Ans✓✓✓ -(t'R)X = (t'R)X-TM
-where (t'r)x is the retention time of the analyte X and TM is the
retention time of an unretained species (mobile phase).
-adjusted retention time is the difference between the retention time
of the analyte minus the dead time. Adjusted retention time refers to
the amount of time the analyte spends interacting with the stationary
phase.
affinity chromatography Ans✓✓✓ -affinity chromatography can be
used in a number of applications, including nucleic acid purification,
protein purification from cell free extracts, and purification from blood.
by using affinity chromatography, one can separate proteins that bind a
certain fragment from proteins that do not bind that specific fragment.
-1) Inject a sample into an initially equilibrated affinity chromatography
column
, -2) only the substances with affinity for the ligand are retained in the
column.
-3) other substances with no affinity for the ligand are eluted from the
column.
bubble cell Ans✓✓✓ -if bubbles form in the mobile phase bottle, it
indicates the mobile phase has become supersaturated with dissolved
air.
-all steps in column chromatography procedures depend on efficient
and uniform contact of solution with the solid support. If air bubbles
form in the resin bed, they limit contact with the resin and impede flow
through the column.
capillary zone electrophoresis Ans✓✓✓ -capillary zone electrophoresis
(CZE) is a technique successfully used for the separation of proteins,
peptides, and nucleic acids. Other applications where CZE may be
useful include analysis of inorganic anions and cations, such as those
typically separated by ion chromatography.
chromatogram Ans✓✓✓ -a time-based graphic record (as a
concentration of eluted materials) of a chromatographic separation.
-as the compounds come out of the column, we measure the intensity
of those peaks. The detector response is a function of time, so we have
a record of when each compound is coming out.
