ASCP CERTIFICATION 2025/2026 QUESTIONS WITH
ANSWERS TAGGED A+
✔✔Colcemid - ✔✔stops dividing cells at metaphase
✔✔Psoralens - ✔✔when exposed to long-wave UV light, can intercalate between
dsDNA and prevent amplification
✔✔What effect does formamide have on melting temperature? - ✔✔lowers melting
temp
✔✔Factor V Leiden involves a base change from: - ✔✔G to A
✔✔Gene associated with Alzheimer's? - ✔✔APOE
✔✔gyrase - ✔✔type of topoisomerase II, relieves strain by causing negative super-
coiling
✔✔Formula for melting temperature? - ✔✔4(G+C) + 2(A+T)
✔✔DNase I - endonuclease that creates - ✔✔a single stranded break (Mn2+) or double
stranded break (Mg2+) in DNA depending on metal cation in reaction mixture
✔✔DEPC-treated water is - ✔✔RNase-free water
✔✔bond between ribose and nitrogenous base? - ✔✔glycosidic
✔✔How many genes in a human? - ✔✔~30,000 genes
✔✔Bases in a human genome? - ✔✔3 billion
✔✔Bromophenol blue - ✔✔allows someone to monitor the progress of molecules
moving through the gel
✔✔Burkitt's Lymphoma - ✔✔t(8;14), t(2;8), t(8;22)
✔✔Mantle Cell Lymphoma - ✔✔t(11;14)
✔✔Follicular Lymphoma - ✔✔t(14;18), t(8;14)
✔✔Promyelocytic lymphoma - ✔✔t(15;17)
✔✔Acute Myeloid leukemia (AML) - ✔✔t(8;21), t(6;9)
ANSWERS TAGGED A+
✔✔Colcemid - ✔✔stops dividing cells at metaphase
✔✔Psoralens - ✔✔when exposed to long-wave UV light, can intercalate between
dsDNA and prevent amplification
✔✔What effect does formamide have on melting temperature? - ✔✔lowers melting
temp
✔✔Factor V Leiden involves a base change from: - ✔✔G to A
✔✔Gene associated with Alzheimer's? - ✔✔APOE
✔✔gyrase - ✔✔type of topoisomerase II, relieves strain by causing negative super-
coiling
✔✔Formula for melting temperature? - ✔✔4(G+C) + 2(A+T)
✔✔DNase I - endonuclease that creates - ✔✔a single stranded break (Mn2+) or double
stranded break (Mg2+) in DNA depending on metal cation in reaction mixture
✔✔DEPC-treated water is - ✔✔RNase-free water
✔✔bond between ribose and nitrogenous base? - ✔✔glycosidic
✔✔How many genes in a human? - ✔✔~30,000 genes
✔✔Bases in a human genome? - ✔✔3 billion
✔✔Bromophenol blue - ✔✔allows someone to monitor the progress of molecules
moving through the gel
✔✔Burkitt's Lymphoma - ✔✔t(8;14), t(2;8), t(8;22)
✔✔Mantle Cell Lymphoma - ✔✔t(11;14)
✔✔Follicular Lymphoma - ✔✔t(14;18), t(8;14)
✔✔Promyelocytic lymphoma - ✔✔t(15;17)
✔✔Acute Myeloid leukemia (AML) - ✔✔t(8;21), t(6;9)
