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1. True or false. Hu- True
mans have 10x
more bacterial
cells than eukary-
otic cells
2. What precau- Unviersal precautions. Means that sample will be treated as if its pathogenic and
tions are used for hazardous
unknown sam-
ples and what
does that mean?
3. What are the two 1. Observation
processes for 2. Differentiation
identification?
4. Process for iden- 1. Morphological characteristics (size, shape)
tification: Obser- 2. Presence of observable motility when viewed under microscope
vation
5. Process for iden- 1. Gram + or -
tification: Differ- 2. Chemical reactions (gas-bubbling, fermentation)
entiation 3. Plating observations on selective or differential plates (changes in color to
sample or surrounding media)
6. pathogenicity def- Ability of microbe to cause disease
inition
7. Virulence defini- EASE of pathogen to infect host and cause disease
tion
8. What are the 5 1. Entry
steps to infection 2. Attachment
, BIOD 171 Module 5
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3. Invasion
4. Evasion
5. Exit and transmission
9. 5 steps to infec- Pathogens use portals of entry like mucus membranes or punctures to gain access
tion: Entry to tissues
10. 5 steps to infec- Pathogen attaches and binds to specific tissue surfaces using adhesion factors
tion: Attachment
11. 5 steps to in- Pathogen invades deeper into tissues securing nutrients for growth and multipli-
fection: Invasion cation:
(and methods) -remain on surface
-reside in cell
-go deeper passing through cells
-pass in between cells
12. 5 steps to infec- Pathogens use mechanisms to evade immune system:
tion: Evasion -Hide by entering host cells (antigen masking, antigen mimicry, antigen varia-
tion)
-Undermine host immune response (avoiding phagocytosis, immune suppres-
sion)
13. How do Make capsule, block lysosome fusion, toxin damage to phagocytic cells
pathogens avoid
phagocytosis
14. How do Breakdown antibodies, infect immune system cells, block signals
pathogens sup-
press immune
system
15. Pathogen covers itself in host factors to avoid detection
, BIOD 171 Module 5
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Antigen masking
definition
16. Antigen mimicry Pathogen masks its antigens by producing surface molecules similar to host's
definition
17. Antigen variation Changing its antigens overtime
definition
18. 5 steps to in- Pathogens use portal of exit to leave and be transmitted to new host:
fection: Exist and -Direct: source and host
transmission -Indirect: airborne, biological vector, mechanical vector
19. Transmission Standard (wash hands, gloves, gown, face shields, disinfect), contact (special
precautions handwashing, gloves, gown, disposables), droplet (mask all times), airborne (n95
AIIR facility)
20. Oxidase test Verifies if bacteria uses cytochrome C oxidase in electron transport chain and uses
oxygen as the final electron acceptor
21. Catalase test and Detects catalase enzyme in bacteria. Hydrogen peroxide is mixed with specimen to
how it works see if it breaks down into water and oxygen. (Water droplet with bubbles inside)
22. Streptococcus Gram positive bacteria, nonmotile, cocci appearing in pairs or chains, negative
characteristics catalase activity (facultative anaerobe), oxidase negative
(gram status,
motility, shape
and
arrangement,
catalase activity,
oxidase)
23. What plate is Blood agar plate
used for strep?