Portage Learning Microbiology BIOD 171 module 3 Questions and Answers
A nanometer is defined as: 10 -9 or one-billionth of a meter
False
True or False: A nanometer is longer than
a micrometer A nanometer is 1000x smaller than a
micrometer
Resolution refers to the distance be-
tween two objects at which the objects
can still be seen as separate. Poor or low
resolution means two or more objects
may appear as one.
Contrast on the other hand is the differ-
Resolution and contrast are two critical ence in light absorbance between two
factors that influence your ability to see objects. Poor contrast gives a high back-
an object. Explain each. ground and makes the visualization of
multiple objects difficult. For instance,
trying to identify 2 dark colored objects
at
night (low light = low contrast) versus the
same 2 objects in the middle of
a sunny afternoon (bright light against 2
dark objects = high contrast).
Objective
Assuming a fixed ocular, identify the part Only the oculars (eyepiece) and the ob-
of the microscope you would adjust to jectives contribute to the magnification of
enhance the magnification of a sample. the sample. Since the eyepiece
is fixed, only the objectives could be al-
tered.
Assuming a constant (non-adjustable)
light source power, identify the part of the
Iris diaphragm
microscope you would adjust to limit the
amount of light entering the microscope.
What is the total magnification on (rel-
ative to your eye) of a sample imaged 60x objective x 10x ocular = 600x mag-
with a 60x objective and a 10x eyepiece? nification
Show your math.
True or False: Staining is often required
, Portage Learning Microbiology BIOD 171 module 3 Questions and Answers
to image a cell that is adherent and flat
A nanometer is defined as: 10 -9 or one-billionth of a meter
False
True or False: A nanometer is longer than
a micrometer A nanometer is 1000x smaller than a
micrometer
Resolution refers to the distance be-
tween two objects at which the objects
can still be seen as separate. Poor or low
resolution means two or more objects
may appear as one.
Contrast on the other hand is the differ-
Resolution and contrast are two critical ence in light absorbance between two
factors that influence your ability to see objects. Poor contrast gives a high back-
an object. Explain each. ground and makes the visualization of
multiple objects difficult. For instance,
trying to identify 2 dark colored objects
at
night (low light = low contrast) versus the
same 2 objects in the middle of
a sunny afternoon (bright light against 2
dark objects = high contrast).
Objective
Assuming a fixed ocular, identify the part Only the oculars (eyepiece) and the ob-
of the microscope you would adjust to jectives contribute to the magnification of
enhance the magnification of a sample. the sample. Since the eyepiece
is fixed, only the objectives could be al-
tered.
Assuming a constant (non-adjustable)
light source power, identify the part of the
Iris diaphragm
microscope you would adjust to limit the
amount of light entering the microscope.
What is the total magnification on (rel-
ative to your eye) of a sample imaged 60x objective x 10x ocular = 600x mag-
with a 60x objective and a 10x eyepiece? nification
Show your math.
True or False: Staining is often required
, Portage Learning Microbiology BIOD 171 module 3 Questions and Answers
to image a cell that is adherent and flat
