Bio 221 Lab Exam 1 UWEC questions with verified
answers
Acidic Dyes Ans✓✓✓ Have a negative charge. Will only stain the
background because the dye is repelled by the cell
Aldehyde or keytone group Ans✓✓✓ Contain an oxygen double
bonded to a carbon
Basic Dyes Ans✓✓✓ Have a positive charge. Will only stain the cell
because the cells are negatively charged so the two are attracted.
Benedict's Reagent Ans✓✓✓ Reacts with the keytone/aldehyde group
to form a colored precipitate. Also used for disaccharide
Biuret test Ans✓✓✓ Reacts with peptide bonds
Blood Auger plate Ans✓✓✓ Not Selective, is differential, Hemolysis
visible in the auger
Carbohydrates Ans✓✓✓ Uses elements Carbon, Hydrogen, and
Oxygen in a 1:2:1 ratio
Defined Media Ans✓✓✓ media in which the exact chemical
composition is known
, Deproteinization Ans✓✓✓ Chromosomal proteins are stripped
Differential Growth Media Ans✓✓✓ Contains substances that reveal
visible growth differences between media.
Disaccharide Ans✓✓✓ Two monosaccharides bonded together
DNA and RNA Ans✓✓✓ What are the two types of Nucleic acids?
EMB Plate Ans✓✓✓ Selective, Differential, Only gram- negative
bacteria grow. These bacteria use lactose fermentation producing
purple colonies with a green metallic sheen. Bacteria that don't have
lactose fermentation remain colorless
Enriched Media Ans✓✓✓ - Contains nutrients that encourage growth
of more fastidious organisms.
Fastidious Bacteria Ans✓✓✓ Bacteria that does not grow well without
specific supplements added to the medium
Glycosidic bond Ans✓✓✓ covalent bond that joins two
monosaccharides together.
answers
Acidic Dyes Ans✓✓✓ Have a negative charge. Will only stain the
background because the dye is repelled by the cell
Aldehyde or keytone group Ans✓✓✓ Contain an oxygen double
bonded to a carbon
Basic Dyes Ans✓✓✓ Have a positive charge. Will only stain the cell
because the cells are negatively charged so the two are attracted.
Benedict's Reagent Ans✓✓✓ Reacts with the keytone/aldehyde group
to form a colored precipitate. Also used for disaccharide
Biuret test Ans✓✓✓ Reacts with peptide bonds
Blood Auger plate Ans✓✓✓ Not Selective, is differential, Hemolysis
visible in the auger
Carbohydrates Ans✓✓✓ Uses elements Carbon, Hydrogen, and
Oxygen in a 1:2:1 ratio
Defined Media Ans✓✓✓ media in which the exact chemical
composition is known
, Deproteinization Ans✓✓✓ Chromosomal proteins are stripped
Differential Growth Media Ans✓✓✓ Contains substances that reveal
visible growth differences between media.
Disaccharide Ans✓✓✓ Two monosaccharides bonded together
DNA and RNA Ans✓✓✓ What are the two types of Nucleic acids?
EMB Plate Ans✓✓✓ Selective, Differential, Only gram- negative
bacteria grow. These bacteria use lactose fermentation producing
purple colonies with a green metallic sheen. Bacteria that don't have
lactose fermentation remain colorless
Enriched Media Ans✓✓✓ - Contains nutrients that encourage growth
of more fastidious organisms.
Fastidious Bacteria Ans✓✓✓ Bacteria that does not grow well without
specific supplements added to the medium
Glycosidic bond Ans✓✓✓ covalent bond that joins two
monosaccharides together.
