Bio 103
Bio 103 Final Exam Questions with
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1. DNA is formed of two such strands that orient in: Opposite
directions
2. you never have two 3' or two 5' on: the same end
3. The numbers (3 and 5) come from the: carbon numbers on the
sugar
4. Chargaff's Rule: (Erwin Chargaff discovered this): Nucleotides
of one strand H-bond (hydrogen bond) With nucls. of the other
strand.
5. H-bonding occurs via these two rules
-known as Complimentary Base Pairing: A of one strand only bonds
with T of the opposite strand
C of one strand only bonds with G of the opposite strand
Bio 103
,6. Thus is a section of DNA, the A and T nucleotides and the C
and G nucleotides are: equal to each other
7. Rosalind Franklin: Used X-ray diffraction to discover the double-
helical structure of DNA.
8. James Watson: Scientists that proved the shape of the
DNA
9. Francis Crick: English biochemist who (with Watson in
1953) helped discover the helical structure of DNA (born in 1916)
10. From parental molecule: original beginning molecule of
DNA that is going to be copied or replicated
11. Two Daughter molecules will form =: two "new" daughter
molecules of DNA result from one parental molecule
12. Semiconservative Replication: Process of taking a
parental DNA molecule and separating the two
strands from each other, and then synthesizing new DNA strands to
compliment each parental strand. The resulting daughter DNA
molecules are made up of one old original strand and one newly
synthesized strand.
,13. There is no DNA molecule that is made up of: wo new
strands or two old strands.
14. The result of semiconservative replication is always a:
daughter molecule that is half old and half new.
15. 1st step in DNA Replication is Helicase: An enzyme that
unwinds the parental DNA by breaking H-bonds that connect the
two strands to each other. This is the enzyme that breaks down the
helix.
16. 2nd step in DNA Replication is Binding Proteins: these
proteins attach to each DNA strand after helicase breaks the H-
bonds and prevents them from re-forming and re-attaching the two
strands back to each other.
17. 3rd step in DNA Replication is Ligase: (to ligate means,
to connect) This enzyme connects short pieces of DNA into long
continuous strands.
18. 4th step in DNA Replication is DNA Polymerase: This is
the actual enzyme that will synthesize the new DNA strands.
19. Unwinding the two parental strands:
, -separate parental strands using Helicase (the unwinder)-
separate parental strands using Helicase: binding proteins attach
to each of the newly separated parental strands to prevent the H-
bonds from re-forming (which would re-connect the 2 parental
strands.
31 Synthesis of "new" DNA using DNA Polymerase: -Read
Parental strands and build complimentary strand to accompany each
one.
-Each strand is done separately.
-Polymerase reads each nucleotide in the parental and adds
into the new growing strand the complimentary nucleotide
32. In effect, polymerase is NOT making an exact copy of each
parental strand, but: a complimentary strand to accompany each
parental strand.
33. The complication that involves DNA Polymerase is this:
following after the Helicase complex starts to read
and build only one strand (the one that started with 3'). Polymerase