1
BIO 103
BIO 103 - Module 4 Exam Questions with
Correct Verified Answers Latest Update
(2025/2026) Guaranteed Pass
1. Nutrients: - made up of chemical elements
- extracellular substances that provide the
microbial cell with materials to build protoplasm
and generate energy
2. macronutrients: nutrients required in relatively larger amounts
3. micronutrients: nutrients required in lesser quantities
4. Culture Medium: any nutrient material prepared/used for the
growth and cultivation of microorganisms in the laboratory
5. none: percentage of solidifying agent in a liquid media
6. inoculum preparation; fermentation test: purpose of a liquid
medium
7. Nutrient Broth; Lactose Broth: examples of liquid media
8. 0.1 - 0.5%: percentage of solidifying agent in a semi-solid media
9. Motility test: purpose of a semi-solid medium
BIO 103
,10. Sulfur Indole Motility (SIM) Medium: examples of semi-solid
media
11. 1.5 - 2.0%: percentage of solidifying agent in a solid media
12. Colony morphology observation; hemolysis and pigmentation
characterization: purpose of a solid medium
13. Nutrient Agar; Blood Agar: examples of solid media
14. Agar: - complex polysaccharide usually derived from red algae
- used as solidifying agent for culture media in Petri plates, slants, and
deeps
- no nutritive value; generally not metabolized by microbes
- not affected by growth of bacteria
15. 100, - 40: Agar liquefies at _____ °C and solidifies at ______ °C
16. Synthetic or Defined Medium: type of culture media with precise
nutrient composition and amounts
17. Glucose Inorganic Salt Phosphate: Examples of synthetic media
18. genetic and specific or precise studies: uses for synthetic
medium
, 24 preparation is time-consuming, microorganisms grow
relatively slow, prepared only for microorganisms with known
nutritional requirements: disadvantages of synthetic medium
25. Complex Medium: - *chemically undefined* medium
- precise chemical composition (kinds) *unknown*
- contains certain similar ingredients but chemical composition varies
slightly from batch to batch
- made from extracts from natural materials
(*beef, blood, casein, yeast, soybeans*)
26. Nutrient Agar, yeast extract: Examples of complex media
27. routine purposes: use for Complex Medium
28. easy to prepare, support rapid growth of most
microorganisms: advantages of complex medium
29. general purpose medium: medium that can support most or
almost all types of species
30. Nutrient Agar, Tryptic Soy Agar, Brain Heart Infusion Agar:
examples of general purpose media
BIO 103
BIO 103 - Module 4 Exam Questions with
Correct Verified Answers Latest Update
(2025/2026) Guaranteed Pass
1. Nutrients: - made up of chemical elements
- extracellular substances that provide the
microbial cell with materials to build protoplasm
and generate energy
2. macronutrients: nutrients required in relatively larger amounts
3. micronutrients: nutrients required in lesser quantities
4. Culture Medium: any nutrient material prepared/used for the
growth and cultivation of microorganisms in the laboratory
5. none: percentage of solidifying agent in a liquid media
6. inoculum preparation; fermentation test: purpose of a liquid
medium
7. Nutrient Broth; Lactose Broth: examples of liquid media
8. 0.1 - 0.5%: percentage of solidifying agent in a semi-solid media
9. Motility test: purpose of a semi-solid medium
BIO 103
,10. Sulfur Indole Motility (SIM) Medium: examples of semi-solid
media
11. 1.5 - 2.0%: percentage of solidifying agent in a solid media
12. Colony morphology observation; hemolysis and pigmentation
characterization: purpose of a solid medium
13. Nutrient Agar; Blood Agar: examples of solid media
14. Agar: - complex polysaccharide usually derived from red algae
- used as solidifying agent for culture media in Petri plates, slants, and
deeps
- no nutritive value; generally not metabolized by microbes
- not affected by growth of bacteria
15. 100, - 40: Agar liquefies at _____ °C and solidifies at ______ °C
16. Synthetic or Defined Medium: type of culture media with precise
nutrient composition and amounts
17. Glucose Inorganic Salt Phosphate: Examples of synthetic media
18. genetic and specific or precise studies: uses for synthetic
medium
, 24 preparation is time-consuming, microorganisms grow
relatively slow, prepared only for microorganisms with known
nutritional requirements: disadvantages of synthetic medium
25. Complex Medium: - *chemically undefined* medium
- precise chemical composition (kinds) *unknown*
- contains certain similar ingredients but chemical composition varies
slightly from batch to batch
- made from extracts from natural materials
(*beef, blood, casein, yeast, soybeans*)
26. Nutrient Agar, yeast extract: Examples of complex media
27. routine purposes: use for Complex Medium
28. easy to prepare, support rapid growth of most
microorganisms: advantages of complex medium
29. general purpose medium: medium that can support most or
almost all types of species
30. Nutrient Agar, Tryptic Soy Agar, Brain Heart Infusion Agar:
examples of general purpose media
