BISC 220 LAB EXAM QUESTIONS AND ANSWERS
Macromolecules 2 and scientific method lab
Proteins are made from - CORRECT ANSWER- amino acids that are linked
together via peptide bonds
The biuret assay indicates whether - CORRECT ANSWER- protein is present in a
solution
A spectrometer measures - CORRECT ANSWER- Absorbance at specific
wavelengths
To help identify protein concentrations in unknown samples using a BSA standard
curve
(BSA = bovine serum albumin)
X-axis = protein concentration
Y-axis = absorbance
To create a BSA standard curve, what is needed - CORRECT ANSWER- Serial
dilutions with known protein concentrations must be included
Macromolecules 1 lab - CORRECT ANSWER- ---------
Objective of macromolecules 1 lab is to - CORRECT ANSWER- identify
structures of common carbohydrates
Understand the purpose of using Benedict’s solution and Lugol's reagent
Determine unknown carbohydrate solutions using above techniques
Macromolecules consist of 4 classes - CORRECT ANSWER- carbohydrates,
proteins, lipids, and nucleic acids
Simple sugars contain - CORRECT ANSWER- carbon, hydrogen, oxygen
, Complex carbohydrates - CORRECT ANSWER- long chains (polysaccharides)
Carbohydrates include - CORRECT ANSWER- Monosaccharides, disaccharides,
and polysaccharides
Monosaccharides - CORRECT ANSWER- single sugar molecules -
Glucose, fructose
Disaccharides - CORRECT ANSWER- Carbohydrates that are made up of two
monosaccharides -
Sucrose, lactose
Polysaccharides - CORRECT ANSWER- Carbohydrates that are made up of more
than two monosaccharides
- starch and other sugars
Benedict's solution - CORRECT ANSWER- reacts with monosaccharides and
some disaccharides
(test for glucose)
+++ = Red precipitate
++ = Orange precipitate
+ = Yellow Precipitate
- = blue precipitate
Lugol's reagent - CORRECT ANSWER- used to detect complex sugars
(starch, cellulose, glycogen)
Positive result = dark blue/black precipitate
Negative results = yellow
Chemical hydrolysis is needed to break down - CORRECT ANSWER- some
disaccharides and polysaccharides
What is chemical hydrolysis - CORRECT ANSWER- heating the molecule with
acid (hcl) to break a glycosidic bond = separates disaccharides and polysaccharides
into monosaccharides
Macromolecules 2 and scientific method lab
Proteins are made from - CORRECT ANSWER- amino acids that are linked
together via peptide bonds
The biuret assay indicates whether - CORRECT ANSWER- protein is present in a
solution
A spectrometer measures - CORRECT ANSWER- Absorbance at specific
wavelengths
To help identify protein concentrations in unknown samples using a BSA standard
curve
(BSA = bovine serum albumin)
X-axis = protein concentration
Y-axis = absorbance
To create a BSA standard curve, what is needed - CORRECT ANSWER- Serial
dilutions with known protein concentrations must be included
Macromolecules 1 lab - CORRECT ANSWER- ---------
Objective of macromolecules 1 lab is to - CORRECT ANSWER- identify
structures of common carbohydrates
Understand the purpose of using Benedict’s solution and Lugol's reagent
Determine unknown carbohydrate solutions using above techniques
Macromolecules consist of 4 classes - CORRECT ANSWER- carbohydrates,
proteins, lipids, and nucleic acids
Simple sugars contain - CORRECT ANSWER- carbon, hydrogen, oxygen
, Complex carbohydrates - CORRECT ANSWER- long chains (polysaccharides)
Carbohydrates include - CORRECT ANSWER- Monosaccharides, disaccharides,
and polysaccharides
Monosaccharides - CORRECT ANSWER- single sugar molecules -
Glucose, fructose
Disaccharides - CORRECT ANSWER- Carbohydrates that are made up of two
monosaccharides -
Sucrose, lactose
Polysaccharides - CORRECT ANSWER- Carbohydrates that are made up of more
than two monosaccharides
- starch and other sugars
Benedict's solution - CORRECT ANSWER- reacts with monosaccharides and
some disaccharides
(test for glucose)
+++ = Red precipitate
++ = Orange precipitate
+ = Yellow Precipitate
- = blue precipitate
Lugol's reagent - CORRECT ANSWER- used to detect complex sugars
(starch, cellulose, glycogen)
Positive result = dark blue/black precipitate
Negative results = yellow
Chemical hydrolysis is needed to break down - CORRECT ANSWER- some
disaccharides and polysaccharides
What is chemical hydrolysis - CORRECT ANSWER- heating the molecule with
acid (hcl) to break a glycosidic bond = separates disaccharides and polysaccharides
into monosaccharides
