MICROBIOLOGY LAB PRACTICAL #2 NEWEST
EXAM 2025 WITH MULTIPLE CHOICE OF
QUESTIONS AND DETAILED SOLVED
SOLUTIONS ALREADY GRADED A+ AND 100%
GUARANTEE PASS (JUST RELEASED!!!!!)
Complete dilution factor (CDF) = Find the product of each
individual dilution for each tube or bottle employed in the
series.
Plate dilution = cumulative (total) dilution x volume plated
Why is E. coli a good choice for a microbe in this experiment? -
CORRECT ANSWER-It's normally found in the colon
After division it does not stick together after division
It's motile and move out from the point of division or origin
It will more likely represent what has come from one cell
instead of a group of cells.
How do you make a lawn of bacteria? What is its purpose? -
CORRECT ANSWER-You need to spread it evenly with no holes
The purpose is to test antibacterial sensitivity.
What is a zone of inhibition? How is it formed? - CORRECT ANSWER-
zone of inhibition: how far around the disk growth is inhibited
(measure the zone)
, It is formed it uses a concentration gradient throughout the
media
What is the relationship between zone of inhibition (ZOI) size
and antibiotic effectiveness? - CORRECT ANSWER-Positive
relationship, the bigger the zone the more effective
Antibiotics
How to evaluate effectiveness of antibiotic & sensitivity of a
bacterium - CORRECT ANSWER-Sensitivity: is the goal for the
Antibiotic (it is what you want)
intermediate: it can still work in killing the bacteria but it is not
encouraged
Resistant: it is not encourage it doesn't killed much if any
bacteria
Kirby-Bauer method: how is it set up and run? What did we do
differently from the original set up? Know how to read Table
33.1. - CORRECT ANSWER--it uses a Mueller Hinton Ager we make a
lawn with the organism to be tested. Then we dispense
Antibiotics equal distance from each other- incubates 18 hours
then you measure the zone
-the only thing we did differently is that we used 4 antibiotics
and some blanks
And we used a Sterile Swab to inoculate the ager
EXAM 2025 WITH MULTIPLE CHOICE OF
QUESTIONS AND DETAILED SOLVED
SOLUTIONS ALREADY GRADED A+ AND 100%
GUARANTEE PASS (JUST RELEASED!!!!!)
Complete dilution factor (CDF) = Find the product of each
individual dilution for each tube or bottle employed in the
series.
Plate dilution = cumulative (total) dilution x volume plated
Why is E. coli a good choice for a microbe in this experiment? -
CORRECT ANSWER-It's normally found in the colon
After division it does not stick together after division
It's motile and move out from the point of division or origin
It will more likely represent what has come from one cell
instead of a group of cells.
How do you make a lawn of bacteria? What is its purpose? -
CORRECT ANSWER-You need to spread it evenly with no holes
The purpose is to test antibacterial sensitivity.
What is a zone of inhibition? How is it formed? - CORRECT ANSWER-
zone of inhibition: how far around the disk growth is inhibited
(measure the zone)
, It is formed it uses a concentration gradient throughout the
media
What is the relationship between zone of inhibition (ZOI) size
and antibiotic effectiveness? - CORRECT ANSWER-Positive
relationship, the bigger the zone the more effective
Antibiotics
How to evaluate effectiveness of antibiotic & sensitivity of a
bacterium - CORRECT ANSWER-Sensitivity: is the goal for the
Antibiotic (it is what you want)
intermediate: it can still work in killing the bacteria but it is not
encouraged
Resistant: it is not encourage it doesn't killed much if any
bacteria
Kirby-Bauer method: how is it set up and run? What did we do
differently from the original set up? Know how to read Table
33.1. - CORRECT ANSWER--it uses a Mueller Hinton Ager we make a
lawn with the organism to be tested. Then we dispense
Antibiotics equal distance from each other- incubates 18 hours
then you measure the zone
-the only thing we did differently is that we used 4 antibiotics
and some blanks
And we used a Sterile Swab to inoculate the ager
