ASCP MLT Chemistry
ASCP MLT CHEMISTRY 2025 EXAM
QUESTIONS AND ANSWERS
Which element in proteins differentiates substance from carbohydrates and lipids?
- nitrogen
What proteins is soluble in water? - albumin
How are amino acids joined together to form a chain like structure? - peptide
bonding
Proteins may become denatured when subjected to? - mechanical agitation, heat,
chemical treatments
What does denaturation of proteins refer to? - alteration in tertiary structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? - cystinuria
How many immunoglobulin classes are currently recognized? - five
which reagent is employed in the serum protein determination? - biuret
What should hemolyzed serum not be used for total protein analysis? - hemoglobin
is absorbed at the same wavelength as protein in the reagent
ASCP MLT Chemistry
,ASCP MLT Chemistry
What is NOT true of the biuret reaction? - is relatively free from interference by
lipids and hemoglobin
Why is bromcresol purple the preferred indicator for albumin dye binding
techniques? - there is less interference from pigment (s)
What is the most anodic protein on electrophoresis at pH 8.6? - albumin
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad
gamma is seen in which disease state? - acute inflammation
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4-10%
beta-globulin, and 45% gamma-globulin, then what condition may exist in the
patient? - monoclonal gammopathy
Glycoproteins and mucoproteins are usually bound to which substance? -
carbohydrates
Name some glycoproteins? - ceruloplasmin, follicle-stimulating hormone,
cryoglobulin
What applies to cryoglobulins? - they are temperature-sensitive proteins
What is true of albumen? - it is produced in the liver and it is water soluble
Decreased alpha-fetoprotein values are associated with what condition? - Down
Syndrome
ASCP MLT Chemistry
, ASCP MLT Chemistry
What protein transports iron? - transferrin
In acute renal failure, what non protein nitrogen rises the fastest? - blood urea
nitrogen
By the urease method, urea is enzymatically converted to which end product? -
ammonia
Why can untreated urine be used for the determination of urea by the diacetyl
monoxime method? - The method is not measureing ammonia
An elevated creatinine value is most likely to be accompanied by what values? -
elevated blood urea nitrogen, 10X creatinine value
What is the classical method for creatinine reaction? - Jaffe
The measurement of creatinine is based on the formation of a yellow-red color and
which reagent? - titan yellow
The creatinine clearance test is based on what assumption? - Creatinine passes into
the ultrafiltrate
What is the most common clearance test used to measure the glomerular filtration
rate? - creatinine
ASCP MLT Chemistry
ASCP MLT CHEMISTRY 2025 EXAM
QUESTIONS AND ANSWERS
Which element in proteins differentiates substance from carbohydrates and lipids?
- nitrogen
What proteins is soluble in water? - albumin
How are amino acids joined together to form a chain like structure? - peptide
bonding
Proteins may become denatured when subjected to? - mechanical agitation, heat,
chemical treatments
What does denaturation of proteins refer to? - alteration in tertiary structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? - cystinuria
How many immunoglobulin classes are currently recognized? - five
which reagent is employed in the serum protein determination? - biuret
What should hemolyzed serum not be used for total protein analysis? - hemoglobin
is absorbed at the same wavelength as protein in the reagent
ASCP MLT Chemistry
,ASCP MLT Chemistry
What is NOT true of the biuret reaction? - is relatively free from interference by
lipids and hemoglobin
Why is bromcresol purple the preferred indicator for albumin dye binding
techniques? - there is less interference from pigment (s)
What is the most anodic protein on electrophoresis at pH 8.6? - albumin
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad
gamma is seen in which disease state? - acute inflammation
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4-10%
beta-globulin, and 45% gamma-globulin, then what condition may exist in the
patient? - monoclonal gammopathy
Glycoproteins and mucoproteins are usually bound to which substance? -
carbohydrates
Name some glycoproteins? - ceruloplasmin, follicle-stimulating hormone,
cryoglobulin
What applies to cryoglobulins? - they are temperature-sensitive proteins
What is true of albumen? - it is produced in the liver and it is water soluble
Decreased alpha-fetoprotein values are associated with what condition? - Down
Syndrome
ASCP MLT Chemistry
, ASCP MLT Chemistry
What protein transports iron? - transferrin
In acute renal failure, what non protein nitrogen rises the fastest? - blood urea
nitrogen
By the urease method, urea is enzymatically converted to which end product? -
ammonia
Why can untreated urine be used for the determination of urea by the diacetyl
monoxime method? - The method is not measureing ammonia
An elevated creatinine value is most likely to be accompanied by what values? -
elevated blood urea nitrogen, 10X creatinine value
What is the classical method for creatinine reaction? - Jaffe
The measurement of creatinine is based on the formation of a yellow-red color and
which reagent? - titan yellow
The creatinine clearance test is based on what assumption? - Creatinine passes into
the ultrafiltrate
What is the most common clearance test used to measure the glomerular filtration
rate? - creatinine
ASCP MLT Chemistry
