ASCP
ASCP MLT CHEMISTRY 2025
QUESTIONS AND ANSWERS
Which element in proteins differentiates substance from carbohydrates and lipids? - nitrogen
What proteins is soluble in water? - albumin
How are amino acids joined together to form a chain like structure? - peptide bonding
Proteins may become denatured when subjected to? - mechanical agitation, heat, chemical
treatments
What does denaturation of proteins refer to? - alteration in tertiary structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? - cystinuria
How many immunoglobulin classes are currently recognized? - five
which reagent is employed in the serum protein determination? - biuret
What should hemolyzed serum not be used for total protein analysis? - hemoglobin is absorbed
at the same wavelength as protein in the reagent
What is NOT true of the biuret reaction? - is relatively free from interference by lipids and
hemoglobin
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Why is bromcresol purple the preferred indicator for albumin dye binding techniques? - there is
less interference from pigment (s)
What is the most anodic protein on electrophoresis at pH 8.6? - albumin
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad gamma is
seen in which disease state? - acute inflammation
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4-10% beta-globulin,
and 45% gamma-globulin, then what condition may exist in the patient? - monoclonal
gammopathy
Glycoproteins and mucoproteins are usually bound to which substance? - carbohydrates
Name some glycoproteins? - ceruloplasmin, follicle-stimulating hormone, cryoglobulin
What applies to cryoglobulins? - they are temperature-sensitive proteins
What is true of albumen? - it is produced in the liver and it is water soluble
Decreased alpha-fetoprotein values are associated with what condition? - Down Syndrome
What protein transports iron? - transferrin
In acute renal failure, what non protein nitrogen rises the fastest? - blood urea nitrogen
By the urease method, urea is enzymatically converted to which end product? - ammonia
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, ASCP
Why can untreated urine be used for the determination of urea by the diacetyl monoxime
method? - The method is not measureing ammonia
An elevated creatinine value is most likely to be accompanied by what values? - elevated blood
urea nitrogen, 10X creatinine value
What is the classical method for creatinine reaction? - Jaffe
The measurement of creatinine is based on the formation of a yellow-red color and which
reagent? - titan yellow
The creatinine clearance test is based on what assumption? - Creatinine passes into the
ultrafiltrate
What is the most common clearance test used to measure the glomerular filtration rate? -
creatinine
Creatinine clearance formula - UV/P
or UV/P (1.73/correction factor)
1440 mls/min
A creatinine clearance test is performed. The 24-hour urine volume is 770 mL; serum creatinine
is 2.0 mg/dL and urine creatinine is 240 mg/dL. What is the clearance, assuming average body
surface area? - 64 mL/minute
What disease state is associated with an elevation of serum uric acid? - gout
ASCP
ASCP MLT CHEMISTRY 2025
QUESTIONS AND ANSWERS
Which element in proteins differentiates substance from carbohydrates and lipids? - nitrogen
What proteins is soluble in water? - albumin
How are amino acids joined together to form a chain like structure? - peptide bonding
Proteins may become denatured when subjected to? - mechanical agitation, heat, chemical
treatments
What does denaturation of proteins refer to? - alteration in tertiary structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? - cystinuria
How many immunoglobulin classes are currently recognized? - five
which reagent is employed in the serum protein determination? - biuret
What should hemolyzed serum not be used for total protein analysis? - hemoglobin is absorbed
at the same wavelength as protein in the reagent
What is NOT true of the biuret reaction? - is relatively free from interference by lipids and
hemoglobin
ASCP
,ASCP
Why is bromcresol purple the preferred indicator for albumin dye binding techniques? - there is
less interference from pigment (s)
What is the most anodic protein on electrophoresis at pH 8.6? - albumin
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad gamma is
seen in which disease state? - acute inflammation
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4-10% beta-globulin,
and 45% gamma-globulin, then what condition may exist in the patient? - monoclonal
gammopathy
Glycoproteins and mucoproteins are usually bound to which substance? - carbohydrates
Name some glycoproteins? - ceruloplasmin, follicle-stimulating hormone, cryoglobulin
What applies to cryoglobulins? - they are temperature-sensitive proteins
What is true of albumen? - it is produced in the liver and it is water soluble
Decreased alpha-fetoprotein values are associated with what condition? - Down Syndrome
What protein transports iron? - transferrin
In acute renal failure, what non protein nitrogen rises the fastest? - blood urea nitrogen
By the urease method, urea is enzymatically converted to which end product? - ammonia
ASCP
, ASCP
Why can untreated urine be used for the determination of urea by the diacetyl monoxime
method? - The method is not measureing ammonia
An elevated creatinine value is most likely to be accompanied by what values? - elevated blood
urea nitrogen, 10X creatinine value
What is the classical method for creatinine reaction? - Jaffe
The measurement of creatinine is based on the formation of a yellow-red color and which
reagent? - titan yellow
The creatinine clearance test is based on what assumption? - Creatinine passes into the
ultrafiltrate
What is the most common clearance test used to measure the glomerular filtration rate? -
creatinine
Creatinine clearance formula - UV/P
or UV/P (1.73/correction factor)
1440 mls/min
A creatinine clearance test is performed. The 24-hour urine volume is 770 mL; serum creatinine
is 2.0 mg/dL and urine creatinine is 240 mg/dL. What is the clearance, assuming average body
surface area? - 64 mL/minute
What disease state is associated with an elevation of serum uric acid? - gout
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