MIC 205A FINAL EXAM QUESTIONS WITH
CORRECT ANSWERS
Hydramacin is a protein that is 60 amino acids long. How many nucleobases would the
mRNA encoding hydramacin have - ANSWER 180
Scientists discovered this novel protein while studying what mechanism in Hydra? -
ANSWER Skin defenses
3Hydramacin is unique, but appears to be most similar to proteins found in - ANSWER
Scorpion venom
the Kirby-Bauer disc diffusion test is used to determine: - ANSWER antimicrobial
susceptibility
During incubation, antimicrobials applied to the bacterial lawn become increasingly
_______ as they diffuse out of the disc into the medium. - ANSWER diluted (The
antimicrobial agents move from a high concentration on the disc to a lower
concentration within the medium via diffusion)
The area of clearing around the discs is called the zone of: - ANSWER inhibition (A zone
of inhibition is the clear area that develops around a test bacterium sensitive to the
applied antimicrobial agent.)
the diameter of the zone of inhibition is always measured in mm. - ANSWER The
diameter of the zone of inhibition is measured in millimeters (mm) to determine
susceptibility to antimicrobial agents.
Which of the medications tested in the above image is a beta-lactam antibiotic? -
ANSWER ampicillin(Ampicillin is a semisynthetic penicillin that targets the cell wall.)
Which of the following medications created the largest zone of inhibition - ANSWER
chloramphenicol (The largest zone of inhibition was measured at 27 mm around the disc
containing chloramphenicol.)
Please evaluate the test organism's sensitivity to chloramphenico - ANSWER
sensitive(The zone of inhibition measured 27 mm for this drug, which is classified as
sensitive to this medication)
What is the test organism's level of sensitivity to tetracycline? - ANSWER intermediate(
The zone of inhibition measured 15 mm for this medication, which is classified as
resistant to this medication. Less than 14 mm indicates resistance to the medication,
while more than 19 mm would indicate sensitivity to the medication. Please refer to
sections 20.1 and 20.4 for additional information)
, Based upon the information in the case, hydramacin can be classified as what type of
antibiotic? - ANSWER broad spectrum ( Hydramacin appears to be effective against
both Gram-positive and Gram-negative bacteria making it a broad spectrum antibiotic.)
If hydramacin exhibits selective toxicity in experiments, it will - ANSWER cause no
damage to host tissues (A medication exhibiting selective toxicity kills or inhibits
microbial cells but does not cause host cells any damage. Please refer to sections 20.1
and 20.4 for aditional information)
Hydramacin needs to be tested in human trials - ANSWER true (One important phase of
testing is to determine if a compound has any toxic effects on the patient.
If the Kirby-Bauer assay revealed any zone of inhibition for hydramacin, this would mean
that the test organism was sensitive to this novel drug. - ANSWER False (False, the
measurement of the diameter of the zone of inhibition would have to be compared to the
established range for these values in order to determine if the organism was in fact
sensitive to hydramacin.
selective toxicity - ANSWER This term is used to describe compounds that cause
greater harm to microbes than to the human host.
antimicrobial action - ANSWER This term is used to describe the molecular method by
which a compound might kill or inhibit a microbe.
spectrum of activity - ANSWER This term is used to describe the range of different
microbes that a compound can kill or inhibit.
Viruses are able to multiply inside a non-living cell. - ANSWER False
Individual viral particles have only one type of nucleic acid. - ANSWER true
Place the following steps of viral replication in their correct order:
1. release 2. adsorption 3. assembly 4. uncoating 5. synthesis - ANSWER 2,4,5,3,1
Cells that lack compatible virus receptors are resistant to infection by that virus. -
ANSWER true
What is the function of the reverse transcriptase enzyme? - ANSWER synthesizes DNA
CORRECT ANSWERS
Hydramacin is a protein that is 60 amino acids long. How many nucleobases would the
mRNA encoding hydramacin have - ANSWER 180
Scientists discovered this novel protein while studying what mechanism in Hydra? -
ANSWER Skin defenses
3Hydramacin is unique, but appears to be most similar to proteins found in - ANSWER
Scorpion venom
the Kirby-Bauer disc diffusion test is used to determine: - ANSWER antimicrobial
susceptibility
During incubation, antimicrobials applied to the bacterial lawn become increasingly
_______ as they diffuse out of the disc into the medium. - ANSWER diluted (The
antimicrobial agents move from a high concentration on the disc to a lower
concentration within the medium via diffusion)
The area of clearing around the discs is called the zone of: - ANSWER inhibition (A zone
of inhibition is the clear area that develops around a test bacterium sensitive to the
applied antimicrobial agent.)
the diameter of the zone of inhibition is always measured in mm. - ANSWER The
diameter of the zone of inhibition is measured in millimeters (mm) to determine
susceptibility to antimicrobial agents.
Which of the medications tested in the above image is a beta-lactam antibiotic? -
ANSWER ampicillin(Ampicillin is a semisynthetic penicillin that targets the cell wall.)
Which of the following medications created the largest zone of inhibition - ANSWER
chloramphenicol (The largest zone of inhibition was measured at 27 mm around the disc
containing chloramphenicol.)
Please evaluate the test organism's sensitivity to chloramphenico - ANSWER
sensitive(The zone of inhibition measured 27 mm for this drug, which is classified as
sensitive to this medication)
What is the test organism's level of sensitivity to tetracycline? - ANSWER intermediate(
The zone of inhibition measured 15 mm for this medication, which is classified as
resistant to this medication. Less than 14 mm indicates resistance to the medication,
while more than 19 mm would indicate sensitivity to the medication. Please refer to
sections 20.1 and 20.4 for additional information)
, Based upon the information in the case, hydramacin can be classified as what type of
antibiotic? - ANSWER broad spectrum ( Hydramacin appears to be effective against
both Gram-positive and Gram-negative bacteria making it a broad spectrum antibiotic.)
If hydramacin exhibits selective toxicity in experiments, it will - ANSWER cause no
damage to host tissues (A medication exhibiting selective toxicity kills or inhibits
microbial cells but does not cause host cells any damage. Please refer to sections 20.1
and 20.4 for aditional information)
Hydramacin needs to be tested in human trials - ANSWER true (One important phase of
testing is to determine if a compound has any toxic effects on the patient.
If the Kirby-Bauer assay revealed any zone of inhibition for hydramacin, this would mean
that the test organism was sensitive to this novel drug. - ANSWER False (False, the
measurement of the diameter of the zone of inhibition would have to be compared to the
established range for these values in order to determine if the organism was in fact
sensitive to hydramacin.
selective toxicity - ANSWER This term is used to describe compounds that cause
greater harm to microbes than to the human host.
antimicrobial action - ANSWER This term is used to describe the molecular method by
which a compound might kill or inhibit a microbe.
spectrum of activity - ANSWER This term is used to describe the range of different
microbes that a compound can kill or inhibit.
Viruses are able to multiply inside a non-living cell. - ANSWER False
Individual viral particles have only one type of nucleic acid. - ANSWER true
Place the following steps of viral replication in their correct order:
1. release 2. adsorption 3. assembly 4. uncoating 5. synthesis - ANSWER 2,4,5,3,1
Cells that lack compatible virus receptors are resistant to infection by that virus. -
ANSWER true
What is the function of the reverse transcriptase enzyme? - ANSWER synthesizes DNA
