Biochem ACS Review questions with verified answers
Does myoglobin have cooperative binding? Ans✓✓✓ NO, there is only one chain
Hemoglobin contains how many subunits? Ans✓✓✓ 4 and each subunit contains
a heme group
How do you determine the Amino Terminus? Ans✓✓✓ 1. Make a derivative of
the N-terminus with a marker molecule
2. Hydrolyze the peptide
3. N-terminal AA is identified by chromatography- modified amino acid will elute
differently than unmodified AA
How does BPG bind to Hb? Ans✓✓✓ There is a binding pocket in deoxy Hb that
consists of positive residues. Negatively charged BPG binds tightly to the pocket.
When Hb is oxygenated, the pocket is too small for BPG
How does Hb transport CO2? Ans✓✓✓ Reaction of CO2 with a -NH2 terminal
amino acids which gives a temporary carbamate
How is CO2 transported out of the cells? Ans✓✓✓ Bicarbonate and Hb
How is the molten globule formed? Ans✓✓✓ It is formed by hydrophobic
collapse. Hydrophobic residues are sequestered toward the inside of a protein
In a Lineweaver-burk plot, what does the slope=? Ans✓✓✓ Km/ Vmax
, In a Lineweaver-burk plot, what does the x-intercept=? Ans✓✓✓ -1/ Km
In a Lineweaver-Burk plot, what does the y-intercept =? Ans✓✓✓ 1/ Vmax
In competitive inhibition, what happens? Ans✓✓✓ the molecule binds to the
active site. Vmax is not altered and the x-intercept changes
In Hb, when are salt linkages formed? Ans✓✓✓ In the Tense form. There is a
lower affinity for O2.
In noncompetitive inhibition, what happens? Ans✓✓✓ the inhibitor binds
somewhere else on the enzyme at the same time the substrate binds. Vmax is
alteredd, y-intercept changes
In Peptide Synthesis, what is the protecting group? Ans✓✓✓ Fmoc
In what order does peptide synthesis, synthesize amino acids? Ans✓✓✓ Carboxy
end to the amine end
In what order does the body synthesize amino acids? Ans✓✓✓ Amino terminus
to carboxy terminus
Myoglobin has mostly what secondary structure? Ans✓✓✓ Alpha helices
The discrepancy in time is answered by what? Ans✓✓✓ Progressive stabilization
Does myoglobin have cooperative binding? Ans✓✓✓ NO, there is only one chain
Hemoglobin contains how many subunits? Ans✓✓✓ 4 and each subunit contains
a heme group
How do you determine the Amino Terminus? Ans✓✓✓ 1. Make a derivative of
the N-terminus with a marker molecule
2. Hydrolyze the peptide
3. N-terminal AA is identified by chromatography- modified amino acid will elute
differently than unmodified AA
How does BPG bind to Hb? Ans✓✓✓ There is a binding pocket in deoxy Hb that
consists of positive residues. Negatively charged BPG binds tightly to the pocket.
When Hb is oxygenated, the pocket is too small for BPG
How does Hb transport CO2? Ans✓✓✓ Reaction of CO2 with a -NH2 terminal
amino acids which gives a temporary carbamate
How is CO2 transported out of the cells? Ans✓✓✓ Bicarbonate and Hb
How is the molten globule formed? Ans✓✓✓ It is formed by hydrophobic
collapse. Hydrophobic residues are sequestered toward the inside of a protein
In a Lineweaver-burk plot, what does the slope=? Ans✓✓✓ Km/ Vmax
, In a Lineweaver-burk plot, what does the x-intercept=? Ans✓✓✓ -1/ Km
In a Lineweaver-Burk plot, what does the y-intercept =? Ans✓✓✓ 1/ Vmax
In competitive inhibition, what happens? Ans✓✓✓ the molecule binds to the
active site. Vmax is not altered and the x-intercept changes
In Hb, when are salt linkages formed? Ans✓✓✓ In the Tense form. There is a
lower affinity for O2.
In noncompetitive inhibition, what happens? Ans✓✓✓ the inhibitor binds
somewhere else on the enzyme at the same time the substrate binds. Vmax is
alteredd, y-intercept changes
In Peptide Synthesis, what is the protecting group? Ans✓✓✓ Fmoc
In what order does peptide synthesis, synthesize amino acids? Ans✓✓✓ Carboxy
end to the amine end
In what order does the body synthesize amino acids? Ans✓✓✓ Amino terminus
to carboxy terminus
Myoglobin has mostly what secondary structure? Ans✓✓✓ Alpha helices
The discrepancy in time is answered by what? Ans✓✓✓ Progressive stabilization
